Detailed information    

insolico Bioinformatically predicted

Overview

Name   cinA   Type   Machinery gene
Locus tag   G8B44_RS08995 Genome accession   NZ_CP049688
Coordinates   1805823..1807094 (-) Length   423 a.a.
NCBI ID   WP_002992182.1    Uniprot ID   A0A660A3W1
Organism   Streptococcus pyogenes strain ABC245     
Function   require for competence (predicted from homology)   
Unclear

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1796099..1824324 1805823..1807094 within 0


Gene organization within MGE regions


Location: 1796099..1824324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8B44_RS08940 (G8B44_09015) nrdG 1796099..1796713 (-) 615 WP_021299115.1 anaerobic ribonucleoside-triphosphate reductase activating protein -
  G8B44_RS08945 (G8B44_09020) - 1796728..1797222 (-) 495 WP_011055085.1 GNAT family N-acetyltransferase -
  G8B44_RS08950 (G8B44_09025) - 1797231..1798166 (-) 936 WP_021299109.1 Gfo/Idh/MocA family protein -
  G8B44_RS08955 (G8B44_09030) - 1798195..1798341 (-) 147 WP_011106921.1 hypothetical protein -
  G8B44_RS08960 (G8B44_09035) nrdD 1798525..1800723 (-) 2199 WP_011529096.1 anaerobic ribonucleoside-triphosphate reductase -
  G8B44_RS08965 (G8B44_09040) - 1800820..1802379 (-) 1560 WP_011055088.1 membrane protein -
  G8B44_RS08970 (G8B44_09045) - 1802793..1803098 (-) 306 WP_002982199.1 DUF1292 domain-containing protein -
  G8B44_RS08975 (G8B44_09050) ruvX 1803110..1803529 (-) 420 WP_002982196.1 Holliday junction resolvase RuvX -
  G8B44_RS08980 (G8B44_09055) - 1803526..1803795 (-) 270 WP_002982194.1 IreB family regulatory phosphoprotein -
  G8B44_RS08985 (G8B44_09060) spx 1803909..1804307 (-) 399 WP_002982188.1 transcriptional regulator Spx -
  G8B44_RS08990 (G8B44_09065) recA 1804598..1805734 (-) 1137 WP_002992179.1 recombinase RecA Machinery gene
  G8B44_RS08995 (G8B44_09070) cinA 1805823..1807094 (-) 1272 WP_002992182.1 competence/damage-inducible protein A Machinery gene
  G8B44_RS09000 (G8B44_09075) - 1807163..1807723 (-) 561 WP_002992183.1 DNA-3-methyladenine glycosylase I -
  G8B44_RS09005 (G8B44_09080) ruvA 1807733..1808329 (-) 597 WP_002992186.1 Holliday junction branch migration protein RuvA -
  G8B44_RS09010 (G8B44_09085) - 1808331..1809551 (-) 1221 WP_011529098.1 MFS transporter -
  G8B44_RS09015 (G8B44_09090) mutL 1809562..1811544 (-) 1983 WP_021299132.1 DNA mismatch repair endonuclease MutL -
  G8B44_RS09020 (G8B44_09095) - 1811639..1812784 (-) 1146 WP_021299091.1 site-specific integrase -
  G8B44_RS09025 (G8B44_09100) - 1812987..1813130 (+) 144 WP_011185066.1 putative holin-like toxin -
  G8B44_RS09030 (G8B44_09105) - 1814028..1814675 (-) 648 WP_011529101.1 helix-turn-helix transcriptional regulator -
  G8B44_RS09035 (G8B44_09110) - 1814841..1815038 (+) 198 WP_011529102.1 helix-turn-helix transcriptional regulator -
  G8B44_RS09040 (G8B44_09115) - 1815052..1815666 (+) 615 WP_021299093.1 Bro-N domain-containing protein -
  G8B44_RS09045 (G8B44_09120) - 1815693..1815935 (+) 243 WP_021299105.1 hypothetical protein -
  G8B44_RS09050 (G8B44_09125) - 1816191..1816523 (+) 333 WP_011529105.1 hypothetical protein -
  G8B44_RS09055 (G8B44_09130) - 1816520..1816741 (+) 222 WP_011529106.1 hypothetical protein -
  G8B44_RS09060 (G8B44_09135) - 1816744..1816935 (+) 192 WP_011529107.1 hypothetical protein -
  G8B44_RS09065 (G8B44_09140) - 1816947..1817309 (+) 363 WP_000206026.1 hypothetical protein -
  G8B44_RS09070 (G8B44_09145) - 1817306..1817635 (+) 330 WP_011529108.1 hypothetical protein -
  G8B44_RS09075 (G8B44_09150) - 1817638..1817910 (+) 273 WP_011529109.1 hypothetical protein -
  G8B44_RS09080 (G8B44_09155) - 1817911..1818771 (+) 861 WP_011529110.1 primase alpha helix C-terminal domain-containing protein -
  G8B44_RS09085 (G8B44_09160) - 1818846..1820285 (+) 1440 WP_042769766.1 phage/plasmid primase, P4 family -
  G8B44_RS09090 (G8B44_09165) - 1820562..1820735 (+) 174 WP_042769761.1 hypothetical protein -
  G8B44_RS09095 (G8B44_09170) - 1820741..1820914 (+) 174 WP_011529113.1 hypothetical protein -
  G8B44_RS09100 (G8B44_09175) - 1820916..1821182 (+) 267 WP_011529114.1 hypothetical protein -
  G8B44_RS09105 (G8B44_09180) - 1821189..1821347 (+) 159 WP_011529115.1 DUF2292 domain-containing protein -
  G8B44_RS09110 (G8B44_09185) - 1821448..1822050 (+) 603 WP_011529116.1 hypothetical protein -
  G8B44_RS09115 (G8B44_09190) - 1822040..1822369 (+) 330 WP_021299097.1 hypothetical protein -
  G8B44_RS09120 (G8B44_09195) - 1822645..1822911 (+) 267 WP_227868883.1 hypothetical protein -
  G8B44_RS09125 (G8B44_09200) - 1823017..1823430 (+) 414 WP_012560493.1 DUF1492 domain-containing protein -
  G8B44_RS09130 (G8B44_09205) - 1823505..1823705 (+) 201 WP_011529120.1 hypothetical protein -
  G8B44_RS09135 (G8B44_09210) - 1823772..1823966 (+) 195 Protein_1755 DUF1492 domain-containing protein -
  G8B44_RS09140 (G8B44_09215) - 1823941..1824324 (+) 384 WP_011285285.1 ArpU family phage packaging/lysis transcriptional regulator -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 45933.64 Da        Isoelectric Point: 4.7648

>NTDB_id=426777 G8B44_RS08995 WP_002992182.1 1805823..1807094(-) (cinA) [Streptococcus pyogenes strain ABC245]
MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGDNEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQT
LAKYLRRDLVYDEQACQKLDDFFAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPI
VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTLRLSTKTENQALADKKLGQLEAQ
LLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKYDKSITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKM
LGLPLEELKSHGVVSAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLISGRSRLDV
CYIATLHAFNMVRKTLLKLENLL

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=426777 G8B44_RS08995 WP_002992182.1 1805823..1807094(-) (cinA) [Streptococcus pyogenes strain ABC245]
ATGAAAGCTGAACTGATTGCAGTAGGTACCGAAATTTTGACTGGTCAAATTGTGAATACCAATGCTCAATTTCTGTCGGA
AAAAATGGCAGAGCTAGGTATTGATGTCTATTTTCAAACGGCTGTTGGGGACAACGAGGAGCGTTTACTTTCAGTGATTA
CAACTGCTAGTCAGCGGAGTGATTTGGTCATTTTATGCGGTGGCCTTGGTCCAACGAAAGATGATTTAACCAAACAAACT
TTAGCAAAGTACCTTAGGAGAGACTTGGTTTATGATGAGCAAGCTTGTCAGAAACTAGATGACTTTTTTGCTAAGCGCAA
GCCTTCATCACGGACACCAAATAATGAGCGACAGGCACAAGTGATTGAAGGATCAATCCCTTTGCCAAATAAAACTGGTC
TTGCGGTTGGTGGGTTCATTACAGTCGATGGTATTAGTTATGTTGTCTTACCGGGTCCTCCAAGTGAATTGAAGCCGATA
GTAAATGAAGAATTGGTACCACTTCTGTCAAAACAATACAGTACATTGTATTCAAAGGTACTACGCTTTTTTGGTATTGG
GGAAAGTCAGTTGGTAACAGTCTTGTCAGATTTTATTGAGAATCAAACTGATCCAACCATTGCTCCGTATGCTAAGACTG
GCGAAGTGACTCTTCGCTTATCAACAAAAACTGAAAACCAAGCTCTGGCAGATAAAAAGTTAGGTCAGCTAGAAGCGCAG
CTACTATCCCGAAAAACTCTTGAAGGTCAACCCTTAGCTGATGTCTTTTATGGCTATGGGGAGGATAATTCCTTAGCGCG
TGAGACATTTGAGCTCTTAGTAAAATATGATAAGTCAATTACAGCAGCAGAAAGTCTAACCGCGGGATTATTTCAGTCAA
CTTTGGCGAGTTTTCCAGGAGCTTCTCAAGTATTCAATGGAGGCTTTGTGACTTATAGCATGGAAGAAAAAGCGAAAATG
CTAGGCCTTCCTTTAGAGGAGTTGAAATCGCATGGCGTTGTTAGTGCTTATACGGCCGAGGGGATGGCGGAGCAAGCAAG
GTTATTGACTGGTGCTGATATTGGGGTAAGTTTAACAGGTGTTGCCGGACCAGATATGTTGGAGGAACAGCCTGCAGGTA
CAGTTTTCATTGGTCTTGCCACTCAAAATAAGGTAGAATCAATAAAGGTTTTGATTAGCGGGCGAAGTCGTTTGGATGTG
TGCTATATCGCTACTTTACATGCCTTTAATATGGTCCGTAAAACTTTATTAAAACTTGAGAATTTGCTATAA

Domains

Predicted by InterproScan.

Probable molybdopterin binding domain (MoCF_biosynth)

(5-170)

Damage-inducible protein CinA KH domain (CinA_KH)

(178-243)

Competence-damaged protein (CinA)

(263-416)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A660A3W1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mutans UA159

69.267

100

0.693
  cinA Streptococcus mitis SK321

69.065

98.582

0.681
  cinA Streptococcus mitis NCTC 12261

68.585

98.582

0.676
  cinA Streptococcus pneumoniae TIGR4

67.626

98.582

0.667
  cinA Streptococcus pneumoniae Rx1

67.626

98.582

0.667
  cinA Streptococcus pneumoniae R6

67.626

98.582

0.667
  cinA Streptococcus pneumoniae D39

67.386

98.582

0.664
  cinA Streptococcus suis isolate S10

52.644

98.345

0.518
  cinA Bacillus subtilis subsp. subtilis str. 168

46.172

98.818

0.456