Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   G5B91_RS05375 Genome accession   NZ_CP049140
Coordinates   1125007..1126224 (-) Length   405 a.a.
NCBI ID   WP_024765634.1    Uniprot ID   -
Organism   Pseudomonas nitroreducens strain HBP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1120007..1131224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5B91_RS05335 (G5B91_05330) - 1120620..1120790 (-) 171 WP_037007365.1 DUF3094 family protein -
  G5B91_RS05340 (G5B91_05335) - 1120885..1121514 (-) 630 WP_024765640.1 DUF1780 domain-containing protein -
  G5B91_RS05345 (G5B91_05340) - 1121636..1122070 (+) 435 WP_037007364.1 hypothetical protein -
  G5B91_RS05350 (G5B91_05345) - 1122067..1122756 (+) 690 WP_024765638.1 energy-coupling factor ABC transporter permease -
  G5B91_RS05355 (G5B91_05350) - 1122863..1123078 (+) 216 WP_024765637.1 hypothetical protein -
  G5B91_RS05360 (G5B91_05355) yacG 1123255..1123461 (-) 207 WP_024765636.1 DNA gyrase inhibitor YacG -
  G5B91_RS05365 (G5B91_05360) coaE 1123458..1124066 (-) 609 WP_024765635.1 dephospho-CoA kinase -
  G5B91_RS05370 (G5B91_05365) pilD 1124131..1125003 (-) 873 WP_038803226.1 prepilin peptidase Machinery gene
  G5B91_RS05375 (G5B91_05370) pilC 1125007..1126224 (-) 1218 WP_024765634.1 type II secretion system F family protein Machinery gene
  G5B91_RS05380 (G5B91_05375) pilB 1126228..1127928 (-) 1701 WP_024765633.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G5B91_RS05385 (G5B91_05380) - 1128155..1128691 (+) 537 WP_024765632.1 pilin -
  G5B91_RS05390 (G5B91_05385) tfpZ 1128787..1129509 (+) 723 WP_235376362.1 TfpX/TfpZ family type IV pilin accessory protein -
  G5B91_RS05400 (G5B91_05395) galE 1129712..1130728 (-) 1017 WP_038803227.1 UDP-glucose 4-epimerase GalE -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44162.02 Da        Isoelectric Point: 10.0743

>NTDB_id=424912 G5B91_RS05375 WP_024765634.1 1125007..1126224(-) (pilC) [Pseudomonas nitroreducens strain HBP1]
MAEKALKTSLFVWEGTDKKGGKIKGELSGVNTALVKAQLRKQGINPTKVRKKGISLLGAGKRIKPMDIALFTRQLATMMQ
AGVPLLQSFDIIGEGFDNPNMRKLVDDVKQEVAAGNSLANSLRKKPQYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
EQLKAKIKKAMTYPIAVIVVAIVVSAILLIKVVPQFQSVFANFGAELPAFTLMVIHMSEAMQAWWFLFLIGAFAIAFVFK
EAHKRSEKFRNFVDRTLLKLPIVGMILYKSSVARFARTLSTTFAAGVPLVEGLDSVAGATGNVVFRNAVNKIKQDVSSGT
QLNFSMRTTGVFPSMAIQMTAIGEESGSLDSMLGKVATFFEEEVDNAVDNLTTLMEPMIMAVLGILVGGLIIAMYLPIFQ
LGNAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=424912 G5B91_RS05375 WP_024765634.1 1125007..1126224(-) (pilC) [Pseudomonas nitroreducens strain HBP1]
ATGGCGGAAAAAGCGTTAAAAACCAGTCTCTTCGTCTGGGAAGGCACCGACAAGAAGGGCGGGAAGATCAAGGGGGAGCT
GTCGGGGGTCAACACTGCGCTGGTCAAGGCGCAATTGCGCAAACAGGGGATCAACCCGACCAAGGTACGCAAGAAAGGCA
TCTCGCTGCTGGGTGCAGGCAAACGCATCAAGCCGATGGACATCGCCCTCTTCACCCGCCAGTTGGCGACCATGATGCAG
GCCGGTGTTCCGCTGCTGCAGTCGTTCGACATCATCGGCGAGGGTTTCGACAACCCCAATATGCGCAAGCTGGTGGATGA
CGTGAAGCAGGAGGTCGCCGCGGGTAACAGCCTGGCCAACTCCCTGCGCAAGAAGCCGCAGTACTTCGACGACCTGTACT
GCAACCTGGTGGACGCCGGCGAGCAATCCGGTGCGCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAACAGCTCAAGGCCAAGATCAAGAAGGCCATGACCTACCCCATCGCGGTCATTGTGGTCGCCATCGTGGTTTCGGCCAT
CCTGCTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTATTCGCCAACTTCGGCGCCGAACTGCCGGCCTTTACCTTGATGG
TGATCCATATGTCCGAAGCGATGCAGGCATGGTGGTTCCTCTTCCTGATCGGCGCCTTCGCCATCGCCTTCGTCTTCAAG
GAAGCGCACAAGCGCTCGGAGAAGTTCCGCAACTTCGTCGACCGCACGCTGCTGAAGCTGCCCATCGTCGGCATGATCCT
CTACAAGTCGTCGGTGGCGCGCTTCGCTCGTACTCTCTCGACGACGTTCGCCGCCGGCGTACCTCTGGTGGAAGGCCTGG
ACTCCGTTGCTGGCGCCACTGGCAACGTCGTGTTCCGTAACGCGGTGAACAAGATCAAGCAGGATGTTTCCAGCGGTACT
CAGCTGAACTTCTCCATGCGTACCACTGGCGTATTCCCCAGCATGGCTATCCAGATGACCGCCATCGGCGAAGAGTCCGG
CTCGCTGGACAGCATGCTGGGCAAGGTGGCGACCTTCTTCGAAGAGGAGGTCGACAACGCCGTCGATAACCTGACCACCC
TGATGGAGCCGATGATCATGGCGGTACTGGGCATATTGGTTGGTGGCCTGATCATTGCCATGTACCTGCCGATCTTCCAA
CTGGGCAACGCCATCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

81.728

100

0.817
  pilC Acinetobacter baumannii D1279779

60

100

0.6
  pilC Acinetobacter baylyi ADP1

58.867

100

0.59
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria meningitidis 44/76-A

44.665

99.506

0.444
  pilG Neisseria gonorrhoeae MS11

44.417

99.506

0.442
  pilC Vibrio cholerae strain A1552

42.893

99.012

0.425
  pilC Vibrio campbellii strain DS40M4

40.051

96.79

0.388
  pilC Thermus thermophilus HB27

36.658

99.012

0.363