Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G5C67_RS03310 Genome accession   NZ_CP049089
Coordinates   613783..615168 (-) Length   461 a.a.
NCBI ID   WP_021113170.1    Uniprot ID   A0A836MFV1
Organism   Glaesserella parasuis strain vHPS7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 608783..620168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5C67_RS03285 (G5C67_03260) clpP 608800..609381 (+) 582 WP_005711766.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  G5C67_RS03290 (G5C67_03265) clpX 609388..610638 (+) 1251 WP_075630799.1 ATP-dependent protease ATP-binding subunit ClpX -
  G5C67_RS03295 (G5C67_03270) dapE 610712..611845 (-) 1134 WP_021113185.1 succinyl-diaminopimelate desuccinylase -
  G5C67_RS03300 (G5C67_03275) - 611929..612597 (-) 669 WP_021113181.1 prepilin peptidase -
  G5C67_RS03305 (G5C67_03280) pilC 612594..613790 (-) 1197 WP_021113189.1 type II secretion system F family protein Machinery gene
  G5C67_RS03310 (G5C67_03285) pilB 613783..615168 (-) 1386 WP_021113170.1 GspE/PulE family protein Machinery gene
  G5C67_RS03315 (G5C67_03290) pilA 615216..615674 (-) 459 WP_021113149.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  G5C67_RS03320 (G5C67_03295) radA 615862..617238 (+) 1377 WP_075606468.1 DNA repair protein RadA -
  G5C67_RS03325 (G5C67_03300) gmk 617291..617917 (-) 627 WP_021113158.1 guanylate kinase -
  G5C67_RS03330 (G5C67_03305) gpt 618155..618634 (-) 480 WP_005714020.1 xanthine phosphoribosyltransferase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52630.33 Da        Isoelectric Point: 4.9724

>NTDB_id=424644 G5C67_RS03310 WP_021113170.1 613783..615168(-) (pilB) [Glaesserella parasuis strain vHPS7]
MSQYTVCEQQTERIFDISPSRWQQNCEEKELLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPFYEESELAFAEQEQEQLNLSDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALSAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCCGTGC
DQCYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=424644 G5C67_RS03310 WP_021113170.1 613783..615168(-) (pilB) [Glaesserella parasuis strain vHPS7]
ATGTCCCAATACACCGTCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAACAAAATTGCGA
AGAAAAAGAGCTGTTATTGCGTTATCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCTGTGGTGATTGCTTCTGATGAA
CTTAAATACTTGCTTAACGCACTCTCGCCTGAGCAACAGCCCTTTTATGAAGAGAGCGAACTGGCTTTCGCAGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAGCCCAAAATGCGT
CCGACATTCATATTGAACCACGCAAGCAAAAATTGATTATTCGCCTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGGCTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACCACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCCTTTACAGCGCCTTAAACTATCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCCGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTGTTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAAACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATCAGCGGTCGAACGTTTGTTACAACTAGGTATTCAAGAGTATG
AAATCCAAAATTCGCTATTGTTAGTGATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTTGTGGCACAGGCTGT
GACCAATGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGCTTAAGCAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGACTTTTCCAGCTTACGAGAGAGTGCCAAACAGAAAATTGAACAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A836MFV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

96.746

100

0.967
  pilB Haemophilus influenzae 86-028NP

57.174

99.783

0.57
  pilB Haemophilus influenzae Rd KW20

57.802

98.698

0.57
  pilB Vibrio cholerae strain A1552

38.554

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

39.004

100

0.408
  pilB Vibrio campbellii strain DS40M4

39.085

100

0.408
  pilB Acinetobacter baylyi ADP1

36.832

100

0.403
  pilB Acinetobacter baumannii D1279779

35.992

100

0.401
  pilB Legionella pneumophila strain ERS1305867

40.682

95.445

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.085

100

0.388
  pilF Neisseria gonorrhoeae MS11

36.421

100

0.375