Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G5C67_RS03315 Genome accession   NZ_CP049089
Coordinates   615216..615674 (-) Length   152 a.a.
NCBI ID   WP_021113149.1    Uniprot ID   K4KA63
Organism   Glaesserella parasuis strain vHPS7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 610216..620674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5C67_RS03295 (G5C67_03270) dapE 610712..611845 (-) 1134 WP_021113185.1 succinyl-diaminopimelate desuccinylase -
  G5C67_RS03300 (G5C67_03275) - 611929..612597 (-) 669 WP_021113181.1 prepilin peptidase -
  G5C67_RS03305 (G5C67_03280) pilC 612594..613790 (-) 1197 WP_021113189.1 type II secretion system F family protein Machinery gene
  G5C67_RS03310 (G5C67_03285) pilB 613783..615168 (-) 1386 WP_021113170.1 GspE/PulE family protein Machinery gene
  G5C67_RS03315 (G5C67_03290) pilA 615216..615674 (-) 459 WP_021113149.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  G5C67_RS03320 (G5C67_03295) radA 615862..617238 (+) 1377 WP_075606468.1 DNA repair protein RadA -
  G5C67_RS03325 (G5C67_03300) gmk 617291..617917 (-) 627 WP_021113158.1 guanylate kinase -
  G5C67_RS03330 (G5C67_03305) gpt 618155..618634 (-) 480 WP_005714020.1 xanthine phosphoribosyltransferase -
  G5C67_RS03335 (G5C67_03310) - 618814..620268 (+) 1455 WP_021113191.1 aminoacyl-histidine dipeptidase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 16069.59 Da        Isoelectric Point: 9.3772

>NTDB_id=424645 G5C67_RS03315 WP_021113149.1 615216..615674(-) (pilA) [Glaesserella parasuis strain vHPS7]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNTRALTNCTGGSNGV
QANKTDVADAKYLKSISVAAGVITVAGKGNIDGYGYTMTPTLANNNITWTTSCRGSDTSLFPANFCGTKATQ

Nucleotide


Download         Length: 459 bp        

>NTDB_id=424645 G5C67_RS03315 WP_021113149.1 615216..615674(-) (pilA) [Glaesserella parasuis strain vHPS7]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTGATGATCGTGATCGCCATCATTGC
TATTTTAGCCACGATTGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCTGCACTCTCTGAGTTATTAAGTGCTTCCG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACACAAGGGCTTTAACTAACTGTACAGGGGGTTCTAACGGCGTT
CAAGCCAATAAAACCGATGTTGCCGATGCTAAATATCTTAAATCTATTTCCGTCGCTGCAGGTGTAATCACAGTGGCTGG
TAAAGGCAACATTGATGGCTACGGCTACACAATGACCCCGACATTAGCCAATAACAACATCACTTGGACAACAAGCTGTC
GAGGTTCTGACACCAGTTTATTCCCTGCGAATTTCTGTGGGACAAAAGCCACACAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)

Pilin (bacterial filament) (Pilin)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K4KA63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

84.967

100

0.855
  pilA Haemophilus influenzae 86-028NP

58.784

97.368

0.572
  pilA Haemophilus influenzae Rd KW20

56.757

97.368

0.553
  comP Acinetobacter baylyi ADP1

44.828

95.395

0.428
  pilE Neisseria gonorrhoeae MS11

36.424

99.342

0.362