Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   G5J76_RS01325 Genome accession   NZ_CP049044
Coordinates   324123..325472 (-) Length   449 a.a.
NCBI ID   WP_046809122.1    Uniprot ID   -
Organism   Pseudomonas psychrophila strain KM02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 319123..330472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5J76_RS01310 (G5J76_01305) - 320782..322341 (-) 1560 WP_019825286.1 AAA family ATPase -
  G5J76_RS01315 (G5J76_01310) aroB 322352..323452 (-) 1101 WP_046809123.1 3-dehydroquinate synthase -
  G5J76_RS01320 (G5J76_01315) aroK 323600..324118 (-) 519 WP_037032209.1 shikimate kinase AroK -
  G5J76_RS01325 (G5J76_01320) pilQ 324123..325472 (-) 1350 WP_046809122.1 type IV pilus secretin PilQ Machinery gene
  G5J76_RS01330 (G5J76_01325) - 325485..326012 (-) 528 WP_019825279.1 pilus assembly protein PilP -
  G5J76_RS01335 (G5J76_01330) - 326009..326632 (-) 624 WP_048352353.1 type 4a pilus biogenesis protein PilO -
  G5J76_RS01340 (G5J76_01335) - 326629..327195 (-) 567 WP_019825276.1 PilN domain-containing protein -
  G5J76_RS01345 (G5J76_01340) pilM 327195..328157 (-) 963 WP_048352352.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 48544.40 Da        Isoelectric Point: 4.7946

>NTDB_id=424366 G5J76_RS01325 WP_046809122.1 324123..325472(-) (pilQ) [Pseudomonas psychrophila strain KM02]
MKRISPVCVLALWSALNSQATLAIATPADTHPSYVGDTMSLNFQEVEVRTVLQLLADFTGFNLVVSDAVQGSITLNLQDV
PWDQALDLVLKSKGLGKRLEGNVLMIAPSDDIAAGERQELEARNQLARLAPLRRELVQVNYAKAADIAKLFQSVTGAQPG
NDGQGTVTVDERTNNIIAFESGERLDELRRLVAQLDVPVRQVMIEARIVEAGVDFEKSLGVRWGGTLRNEGRWSAGGITR
SGGSEVAPGNLGAPFVDLGVSGNTSGLGIAFITNNVLLDLELTAMEKTGNGEIISQPKVVTSDKETAKILKGTEIPYQES
SSSGATSVSFKEASLSLEVTPQITPDNSVIMEVVVTKDEPDYMNRVQDVPPIKKNEVRAKVLVRDGETIVIGGVFSNTQS
KVVDKVPFLGDLPYVGRLFRRDVVLEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=424366 G5J76_RS01325 WP_046809122.1 324123..325472(-) (pilQ) [Pseudomonas psychrophila strain KM02]
ATGAAAAGGATTTCACCGGTCTGCGTACTGGCGCTATGGAGTGCGCTGAATTCGCAGGCAACGCTGGCGATTGCTACGCC
TGCAGACACCCACCCCTCGTATGTCGGCGACACAATGTCCCTTAACTTTCAGGAGGTTGAAGTGCGCACAGTGCTTCAGC
TACTGGCAGATTTTACGGGGTTCAATCTGGTGGTCAGCGACGCTGTTCAAGGCAGCATTACCCTCAACCTGCAGGACGTA
CCCTGGGATCAGGCGCTGGATCTGGTGCTAAAGAGCAAGGGTCTGGGCAAGCGCCTTGAGGGCAATGTGCTGATGATCGC
CCCCTCTGACGACATCGCAGCGGGTGAGCGGCAAGAGCTTGAGGCCCGCAATCAACTGGCCCGACTTGCGCCTTTGCGCA
GAGAATTGGTGCAGGTCAATTACGCCAAGGCAGCAGATATTGCCAAATTGTTCCAGTCGGTGACCGGCGCTCAACCTGGT
AATGACGGGCAGGGCACTGTCACGGTGGACGAACGCACCAATAACATCATCGCCTTTGAGTCTGGAGAGCGGCTGGATGA
GTTGCGACGCCTCGTGGCTCAACTGGATGTTCCGGTACGTCAGGTCATGATCGAGGCCCGGATTGTTGAGGCGGGCGTGG
ACTTCGAGAAAAGCCTGGGGGTTCGTTGGGGTGGAACACTGCGCAACGAAGGCCGCTGGAGTGCGGGCGGCATTACCCGC
TCGGGCGGGTCAGAGGTCGCGCCCGGAAATCTGGGTGCGCCCTTTGTGGACCTCGGCGTTTCAGGCAATACCTCCGGGCT
GGGTATCGCCTTTATCACCAACAATGTGTTGTTGGACCTGGAACTGACGGCCATGGAAAAAACCGGCAATGGCGAAATCA
TTTCTCAGCCCAAAGTAGTTACCTCCGACAAGGAAACCGCCAAAATCCTCAAAGGCACTGAAATTCCCTATCAAGAGTCC
AGTTCAAGCGGGGCCACATCGGTATCGTTCAAGGAGGCCTCGTTGTCCCTGGAAGTGACGCCGCAAATAACCCCGGACAA
CAGCGTCATTATGGAGGTCGTCGTTACCAAGGATGAGCCCGACTACATGAACAGGGTTCAGGATGTGCCACCCATCAAGA
AGAATGAGGTCAGGGCCAAGGTGCTGGTCAGGGACGGTGAAACCATCGTGATTGGGGGAGTGTTCTCAAATACTCAAAGC
AAAGTAGTAGATAAAGTGCCATTTTTAGGAGATCTGCCGTATGTTGGCCGACTTTTCCGTAGAGACGTTGTCCTGGAAAA
AAAATCCGAACTGCTGGTGTTCCTGACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(136-201)

Bacterial type II and III secretion system protein (Secretin)

(284-440)

Secretin and TonB N terminus short domain (STN)

(61-108)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

68.721

97.55

0.67
  pilQ Acinetobacter baumannii strain A118

41.566

100

0.461
  pilQ Acinetobacter baumannii D1279779

41.566

100

0.461
  comQ Acinetobacter baylyi ADP1

43.83

100

0.459
  pilQ Legionella pneumophila strain ERS1305867

44.208

94.209

0.416
  pilQ Legionella pneumophila strain Lp02

44.208

94.209

0.416
  pilQ Vibrio campbellii strain DS40M4

42.689

94.432

0.403
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.608

94.209

0.392
  pilQ Vibrio cholerae strain A1552

41.608

94.209

0.392