Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G3580_RS13235 Genome accession   NZ_CP048836
Coordinates   2842650..2844368 (+) Length   572 a.a.
NCBI ID   WP_173766227.1    Uniprot ID   A0A6C1B639
Organism   Nitrogeniibacter mangrovi strain M9-3-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2837650..2849368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3580_RS13215 (G3580_13220) - 2838171..2838977 (-) 807 WP_173766222.1 ferritin-like domain-containing protein -
  G3580_RS13220 (G3580_13225) ffh 2838985..2840340 (-) 1356 WP_173766224.1 signal recognition particle protein -
  G3580_RS13225 (G3580_13230) - 2840379..2841206 (+) 828 WP_173766226.1 inner membrane protein YpjD -
  G3580_RS13230 (G3580_13235) - 2841281..2842549 (+) 1269 WP_228720662.1 HlyC/CorC family transporter -
  G3580_RS13235 (G3580_13240) pilB 2842650..2844368 (+) 1719 WP_173766227.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G3580_RS13240 (G3580_13245) pilC 2844387..2845622 (+) 1236 WP_173766229.1 type II secretion system F family protein Machinery gene
  G3580_RS13245 (G3580_13250) pilD 2845660..2846502 (+) 843 WP_228720663.1 A24 family peptidase Machinery gene
  G3580_RS13250 (G3580_13255) - 2846603..2846866 (+) 264 WP_173766231.1 FmdB family zinc ribbon protein -
  G3580_RS13255 (G3580_13260) - 2846874..2847506 (+) 633 WP_173766233.1 DUF502 domain-containing protein -
  G3580_RS13260 (G3580_13265) aspS 2847557..2849353 (+) 1797 WP_173766235.1 aspartate--tRNA ligase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62340.16 Da        Isoelectric Point: 4.9047

>NTDB_id=423448 G3580_RS13235 WP_173766227.1 2842650..2844368(+) (pilB) [Nitrogeniibacter mangrovi strain M9-3-2]
MAANPQIALSGLARALIQQNRISEADAVACTTENKDKTNGFVLEAAKRGLMSEHEVARFAADTFGYPLLDIGALDPQTIL
QDAIDRKLIGKHHVLPIGKRGNRITVALADPTNLRALDEIRFQSGLSVDAVVVEASKLEAVINRQSESTSETLDALTGDD
FDMDLLDQESPGDKDGGEDVQAEVDDAPVVRFIQKVLVDAINEGASDIHFEPYEKYYRIRFRTDGALREIAQPPLILREK
IAARIKVISRLDISEKRVPQDGRMKLVLSKNKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGIDALGYDPDQKQALLEAI
ERPYGMILVTGPTGSGKTVSLYTCLNLLNKGDSNISTAEDPAEINLPGINQVNVNEKAGLTFSTALRAFLRQDPDIIMVG
EIRDLETAEISIKAAQTGHLVLSTLHTNDAPTTLERLRNMGVAPFNIASSVILITAQRLARRLCKCKEPMDIPHDALVEA
GFSEDDLDGSWRPMGPKGCDLCKGSGYKGRVGIYQVMPISEEIQRIIMNNGNSMDIAAQAELEGVSDLRRSGLRKVKAGI
TSLSEVLATTND

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=423448 G3580_RS13235 WP_173766227.1 2842650..2844368(+) (pilB) [Nitrogeniibacter mangrovi strain M9-3-2]
ATGGCCGCGAATCCGCAAATTGCGCTGTCCGGGCTCGCCCGGGCGCTGATCCAGCAGAACCGCATCTCGGAAGCGGACGC
GGTCGCGTGCACCACCGAAAACAAGGACAAGACCAACGGCTTCGTCCTCGAGGCGGCCAAACGCGGCCTCATGAGCGAGC
ATGAGGTCGCCCGCTTCGCCGCCGACACCTTCGGCTACCCGCTCCTCGACATCGGCGCGCTGGATCCGCAGACGATCCTG
CAGGACGCCATCGACCGCAAACTCATCGGCAAACACCACGTCCTGCCCATCGGCAAGCGCGGCAATCGCATCACGGTCGC
CCTGGCCGACCCCACGAACCTGAGGGCCCTGGACGAGATCCGCTTCCAGAGCGGCCTGTCGGTCGACGCCGTGGTGGTCG
AGGCGAGCAAGCTCGAGGCGGTCATCAACCGCCAGAGCGAGTCCACGTCAGAGACGCTCGACGCACTGACCGGCGACGAC
TTCGACATGGACCTGCTCGATCAGGAATCGCCGGGCGACAAGGACGGCGGCGAGGATGTCCAGGCCGAGGTGGACGACGC
GCCGGTGGTCCGTTTCATCCAGAAAGTGCTCGTGGACGCCATCAACGAAGGCGCCTCCGACATCCACTTCGAGCCCTATG
AAAAGTATTACCGGATCCGCTTTCGTACCGACGGCGCCCTGCGTGAAATCGCCCAGCCACCGCTGATCCTGCGTGAAAAG
ATCGCCGCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCCGAGAAGCGGGTCCCCCAGGACGGGCGCATGAAGCTGGT
GCTGTCCAAGAACAAGGCCATCGACTTCCGGGTCTCCACCCTGCCGACGCTGCATGGCGAGAAGATCGTGATGCGGATTC
TCGATCCGTCGTCGGCCATGCTGGGCATCGACGCCCTCGGCTACGATCCGGATCAGAAGCAGGCCCTGCTCGAGGCCATC
GAGCGCCCCTACGGCATGATTCTGGTCACCGGCCCGACCGGTAGCGGCAAGACCGTGTCGCTCTACACCTGCCTGAACCT
GCTCAACAAGGGCGACTCGAACATTTCGACCGCCGAAGACCCGGCCGAAATCAACCTGCCCGGCATCAACCAGGTCAATG
TGAACGAGAAGGCGGGCCTGACCTTCTCCACGGCGCTGCGCGCCTTCCTTCGCCAGGATCCCGACATCATCATGGTGGGC
GAGATCCGCGATCTGGAGACCGCGGAGATTTCGATCAAGGCCGCGCAGACCGGTCACCTAGTCCTGTCGACGCTGCATAC
CAACGACGCCCCGACGACGCTCGAGCGCCTGCGCAACATGGGGGTCGCGCCGTTCAACATCGCCTCGTCAGTCATCCTCA
TCACGGCGCAGCGACTGGCCCGCCGCCTGTGCAAGTGCAAGGAACCCATGGACATTCCCCACGACGCGCTGGTCGAGGCG
GGTTTTTCCGAGGACGATCTGGACGGCAGCTGGCGCCCGATGGGCCCGAAGGGCTGCGACCTGTGCAAGGGCTCCGGCTA
CAAGGGACGGGTGGGCATCTACCAGGTCATGCCGATTTCCGAAGAGATTCAGCGCATCATCATGAACAACGGCAATTCCA
TGGACATTGCCGCCCAGGCCGAACTGGAAGGGGTCTCCGATCTGCGCCGCTCCGGGCTGCGCAAAGTGAAGGCTGGCATC
ACCTCGCTATCCGAAGTGCTGGCCACCACCAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-147)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6C1B639

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

99.476

0.554
  pilB Acinetobacter baylyi ADP1

54.77

98.951

0.542
  pilF Neisseria gonorrhoeae MS11

53.076

99.476

0.528
  pilB Legionella pneumophila strain ERS1305867

52.265

100

0.524
  pilB Vibrio cholerae strain A1552

51.638

90.734

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

49.336

92.133

0.455
  pilB Vibrio campbellii strain DS40M4

49.333

91.783

0.453
  pilF Thermus thermophilus HB27

39.194

95.455

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.583

92.308

0.365
  pilB/pilB1 Synechocystis sp. PCC 6803

41.851

86.888

0.364