Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G4G71_RS27610 Genome accession   NZ_CP048833
Coordinates   6050605..6052305 (+) Length   566 a.a.
NCBI ID   WP_169941841.1    Uniprot ID   -
Organism   Pseudomonas multiresinivorans strain populi     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6045605..6057305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4G71_RS27585 (G4G71_27545) nadC 6046858..6047703 (+) 846 WP_169941834.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G4G71_RS27590 (G4G71_27550) galE 6047807..6048823 (+) 1017 WP_169941836.1 UDP-glucose 4-epimerase GalE -
  G4G71_RS27600 (G4G71_27560) - 6049025..6049714 (-) 690 WP_169941838.1 hypothetical protein -
  G4G71_RS27605 (G4G71_27565) - 6049829..6050380 (-) 552 WP_169941840.1 pilin -
  G4G71_RS27610 (G4G71_27570) pilB 6050605..6052305 (+) 1701 WP_169941841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G4G71_RS27615 (G4G71_27575) pilC 6052309..6053526 (+) 1218 WP_205896252.1 type II secretion system F family protein Machinery gene
  G4G71_RS27620 (G4G71_27580) pilD 6053530..6054402 (+) 873 WP_169941842.1 prepilin peptidase Machinery gene
  G4G71_RS27625 (G4G71_27585) coaE 6054467..6055075 (+) 609 WP_169941843.1 dephospho-CoA kinase -
  G4G71_RS27630 (G4G71_27590) yacG 6055072..6055278 (+) 207 WP_024765636.1 DNA gyrase inhibitor YacG -
  G4G71_RS27635 (G4G71_27595) - 6055351..6055566 (-) 216 WP_054910366.1 hypothetical protein -
  G4G71_RS27640 (G4G71_27600) - 6055673..6056362 (-) 690 WP_169941844.1 energy-coupling factor ABC transporter permease -
  G4G71_RS27645 (G4G71_27605) - 6056359..6056793 (-) 435 WP_169942837.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62477.47 Da        Isoelectric Point: 5.5882

>NTDB_id=423411 G4G71_RS27610 WP_169941841.1 6050605..6052305(+) (pilB) [Pseudomonas multiresinivorans strain populi]
MNDQVVLSGLARQLVISELLDEKSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAAEQFGVAFCDLTSLDRESFPKE
LVSEKLVRQHRVLPLWRRGNKLFVGVSDPTNHQAITDVQFSTGLTTEAILVEDDKLGDAIEKLFESATGGLDDLGDIDLD
GLDIENANTEAKEETGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLSSRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEAQKQLYLDALKQPQG
MILVTGPTGSGKTVSLYTGINILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPHCKKEHDVPQEALLHEGFPA
DKIGTFKLYSPVGCDNCKGGYKGRLGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=423411 G4G71_RS27610 WP_169941841.1 6050605..6052305(+) (pilB) [Pseudomonas multiresinivorans strain populi]
ATGAACGATCAGGTCGTATTGTCCGGTCTTGCCCGGCAACTGGTAATTTCAGAACTGCTCGATGAGAAAAGCGCCCAACA
AGCGCAACAGCAGGCCCAGCGCAACAAGCTGTCGCTGGTCACTTACCTGGTACAGAACAAGCTGGTAAAAAGCCGCCCCC
TGACCGAGCTGGCGGCCGAGCAGTTCGGCGTGGCCTTCTGCGATCTAACCAGCCTGGACCGTGAGAGTTTTCCCAAGGAG
TTGGTGAGTGAGAAGCTGGTCCGACAGCATCGCGTACTTCCACTCTGGCGCAGGGGCAACAAGCTGTTCGTCGGCGTTTC
CGATCCGACAAACCATCAAGCCATTACCGATGTCCAGTTCAGCACCGGCCTGACCACCGAGGCCATCCTCGTTGAAGACG
ACAAACTCGGCGATGCCATCGAAAAACTGTTCGAAAGCGCTACCGGAGGGCTGGACGACCTGGGCGACATCGACCTCGAT
GGCCTGGATATCGAGAACGCCAACACCGAGGCGAAGGAAGAGACAGGTGGCGATGCTGACGACGCACCGGTGGTCCGCTT
CGTCAACAAGATGCTGCTGGACGCTATCAAGGGGGGCTCGTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGCG
TACGCTTCCGTACCGACGGCATGTTGCATGAAGTGGCCAAGCCACCCATTCAGTTGTCCAGCCGAATCTCCGCACGCCTG
AAGGTCATGGCCGGCCTGGACATTTCCGAGCGCCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACGCTGCCAACCCTATGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGATGCGCTGGGCTACGAGGAAGCACAGAAGCAACTCTACCTGGACGCACTCAAGCAGCCCCAGGGC
ATGATTCTGGTGACGGGCCCTACCGGCTCGGGCAAGACCGTATCGCTCTATACCGGCATCAACATCCTCAATACGCCGGA
TATCAATATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTGAACCCGCGCCAGG
GCATGGACTTCGCCCAGGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGACCTG
GAAACCGCCGAGATTGCCATCAAGGCCGCGCAGACCGGCCACATGGTGATGTCCACCCTGCACACCAACAGTGCCGCCGA
AACGCTGACCCGCCTGCTGAACATGGGCGTCGCCTCGTTCAACCTGGCCACCTCGACCAACCTGATCATTGCCCAGCGCC
TGGCGCGCAAGCTCTGTCCGCACTGCAAGAAGGAACACGATGTTCCGCAGGAAGCGCTTCTTCATGAAGGCTTCCCGGCC
GACAAGATCGGGACGTTCAAGCTGTATTCCCCCGTCGGGTGCGACAATTGCAAGGGTGGATACAAGGGGCGCTTGGGTAT
TTATGAAGTGGTTAAAATCACGCCAGCGCTGCAACGCATTATCATGGAGGAAGGCAACTCCATTCAGATCGCGGAGCAAG
CCCGCAAAGAAGGCTTCAATGACCTGCGCACCTCGGGCCTGCTCAAGGCCATGCAAGGCATCACCAGCCTCGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.016

99.47

0.567
  pilB Acinetobacter baylyi ADP1

56.94

99.293

0.565
  pilB Legionella pneumophila strain ERS1305867

54.145

100

0.542
  pilB Vibrio cholerae strain A1552

51.159

99.117

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

52.311

95.583

0.5
  pilF Neisseria gonorrhoeae MS11

50

100

0.5
  pilB Vibrio campbellii strain DS40M4

52.319

95.23

0.498
  pilF Thermus thermophilus HB27

38.421

100

0.387
  pilB/pilB1 Synechocystis sp. PCC 6803

39.313

92.58

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.752

93.463

0.362

Multiple sequence alignment