Detailed information NTG0422221

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: G0030_RS01310
• Genome accession: NZ_CP048681
• Coordinates: 280252..281310 (+)
• Length: 352 a.a.
• NCBI ID: WP_016658445.1
• Uniprot ID: V2UHB5
• Organism: Acinetobacter indicus strain Q186-3_T
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 275252..286310

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G0030_RS01295 (G0030_01295)	mraY	275474..276592 (+)	1119	WP_005180530.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
G0030_RS01300 (G0030_01300)	-	276658..277461 (+)	804	WP_180182075.1	putative RNA methyltransferase	-
G0030_RS01305 (G0030_01305)	ponA	277504..280080 (-)	2577	WP_180182074.1	penicillin-binding protein PBP1a	-
G0030_RS01310 (G0030_01310)	pilM	280252..281310 (+)	1059	WP_016658445.1	pilus assembly protein PilM	Machinery gene
G0030_RS01315 (G0030_01315)	pilN	281310..281945 (+)	636	WP_180182073.1	PilN domain-containing protein	Machinery gene
G0030_RS01320 (G0030_01320)	pilO	281942..282661 (+)	720	WP_005180513.1	type 4a pilus biogenesis protein PilO	Machinery gene
G0030_RS01325 (G0030_01325)	pilP	282661..283188 (+)	528	WP_104489345.1	pilus assembly protein PilP	Machinery gene
G0030_RS01330 (G0030_01330)	pilQ	283249..285387 (+)	2139	WP_180182089.1	type IV pilus secretin PilQ	Machinery gene
G0030_RS01335 (G0030_01335)	aroK	285424..285966 (+)	543	WP_075167858.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38940.60 Da
• Isoelectric Point: 4.5587

>NTDB_id=422221 G0030_RS01310 WP_016658445.1 280252..281310(+) (pilM) [Acinetobacter indicus strain Q186-3_T]
MRRLYRKPSKGLIGVDISSTSVKLLELSVKNDRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAFNLANTPTTAAAL
AIPTSMVISKTIEMDADMTDDEREVQIRMDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRLENVESRCEAIEL
AGLTPKVADVESFAIENAFKVFADTLPMGVNTIGVLDIGHTMTTLSVLKDNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDVEVLEPFLEAVVQQAARSLQFFFSSSQFSEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSSKIDIKRIESDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=422221 G0030_RS01310 WP_016658445.1 280252..281310(+) (pilM) [Acinetobacter indicus strain Q186-3_T]
GTGCGCAGGTTATATCGTAAACCGAGTAAGGGGTTAATAGGTGTAGATATTAGTTCTACTTCTGTTAAGTTATTGGAGCT
CTCTGTAAAGAATGATCGTTACTGGGTGGAAAGTTACGCCTTGGTACCGCTCCCAGAGGGCAGTGTGGTTGAGAAAAATA
TTCTGAATCCTGAAGCTGTAGGGGATGCGCTCGAGCGTGCCTTTAATTTGGCCAATACACCGACAACAGCTGCCGCTTTA
GCGATTCCAACCTCAATGGTAATTAGCAAGACCATTGAGATGGATGCCGATATGACGGATGACGAACGTGAAGTTCAAAT
CCGGATGGATGCAGAGCAATATATTCCTTTCCCATTAGATGAAGTTAGCCTGGACTTTGAGGTGCTGCCTGATCGTCTGG
CCAATCCAAACCGGGTCAATGTTTTGCTGGTGGCGACACGACTGGAAAATGTCGAGAGTCGCTGTGAAGCGATTGAACTG
GCCGGTTTAACCCCAAAAGTGGCAGATGTCGAAAGCTTTGCCATTGAAAATGCCTTTAAAGTCTTCGCCGATACCTTGCC
GATGGGGGTAAATACCATTGGTGTGTTAGACATCGGTCATACCATGACCACGCTGTCGGTACTCAAAGACAATAAAATTA
TTTATACCCGCGAGCAGGTCTTTGGTGGCAAGCAGCTGACCCAAGAAATCCAGAACCGTTATGGTCTGTCCTTTGAAGAA
GCCGGCCGCGCCAAGAAAAGCCGTACCTTGCCAGATGATTACGATGTTGAAGTGCTGGAGCCATTCCTTGAGGCGGTGGT
GCAGCAGGCAGCACGTTCGCTGCAGTTCTTCTTCTCTTCATCGCAGTTTAGTGAAATTGACCATATTCTGCTGGCCGGTG
GGAATGCCAATATTCCAGGCCTGGCCAAGCTACTACAGCAAAAACTGGGTTACCGCGTCACCATTGCCAATCCGTTTTTA
CAGATGGGTTTCTCCTCCAAAATTGATATTAAAAGAATTGAAAGCGATGCCTCTTCATTGATGGTGGCATGTGGCTTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: V2UHB5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	84.091	100	0.841
comM	Acinetobacter nosocomialis M2	84.091	100	0.841
comM	Acinetobacter baylyi ADP1	78.409	100	0.784
pilM	Legionella pneumophila strain ERS1305867	41.243	100	0.415