Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G0029_RS14470 Genome accession   NZ_CP048670
Coordinates   2974603..2976312 (+) Length   569 a.a.
NCBI ID   WP_078192562.1    Uniprot ID   -
Organism   Acinetobacter sp. YH12138 strain YH12138_T     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2969603..2981312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0029_RS14425 (G0029_14410) nusA 2970218..2971702 (-) 1485 WP_078192558.1 transcription termination factor NusA -
  G0029_RS14430 (G0029_14415) rimP 2971738..2972262 (-) 525 WP_078192559.1 ribosome maturation factor RimP -
  G0029_RS14460 (G0029_14445) secG 2973193..2973522 (-) 330 WP_180084539.1 preprotein translocase subunit SecG -
  G0029_RS14465 (G0029_14450) tpiA 2973536..2974327 (-) 792 WP_180084538.1 triose-phosphate isomerase -
  G0029_RS14470 (G0029_14455) pilB 2974603..2976312 (+) 1710 WP_078192562.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G0029_RS14475 (G0029_14460) pilC 2976345..2977571 (+) 1227 WP_078192563.1 type II secretion system F family protein Machinery gene
  G0029_RS14480 (G0029_14465) pilD 2977571..2978431 (+) 861 WP_180106794.1 prepilin peptidase Machinery gene
  G0029_RS14485 (G0029_14470) coaE 2978441..2979043 (+) 603 WP_180106792.1 dephospho-CoA kinase -
  G0029_RS14490 (G0029_14475) - 2979058..2979978 (-) 921 WP_372491165.1 DMT family transporter -
  G0029_RS14495 (G0029_14480) rlmB 2979987..2980745 (-) 759 WP_180106790.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  G0029_RS14500 (G0029_14485) - 2980922..2981245 (-) 324 WP_067763086.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63625.63 Da        Isoelectric Point: 4.8371

>NTDB_id=422210 G0029_RS14470 WP_078192562.1 2974603..2976312(+) (pilB) [Acinetobacter sp. YH12138 strain YH12138_T]
MTAVQGSSRFTGFIRRLVDEGHLSAEKMQAALQNSKKAHQDIVPHLIQEYHLSARLIAETISIEFGEPVFDISTYDTSLI
IRDEFEDKLITKHRILPIFKHGNLLFVAISNPTNIEAVDAIRFGSKLNTETIIVEHDKLEKLIEQNFAEESTFDFDDDFD
LDLEQETIDPAKEDEDEATGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKTYRVRYRVDGVLRTITNPPLQLATRLASR
LKVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFMQALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKETVDVPKQSLLELGFT
EDDLNTPEFQIYQPVGCAECREGYKGRVGIYEVMKVTPEISRIIMEDGNALQIAEASNQAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=422210 G0029_RS14470 WP_078192562.1 2974603..2976312(+) (pilB) [Acinetobacter sp. YH12138 strain YH12138_T]
ATGACAGCAGTTCAAGGTTCTTCAAGATTTACGGGGTTTATTCGTCGCTTAGTTGACGAAGGGCATCTGTCTGCCGAAAA
AATGCAAGCAGCGCTGCAAAATTCAAAAAAGGCCCATCAAGATATCGTGCCGCATTTGATTCAGGAATATCACCTTTCAG
CACGCCTGATCGCAGAAACCATCTCGATAGAGTTCGGTGAACCGGTTTTTGATATTTCAACTTATGATACGTCTTTAATT
ATACGTGATGAATTTGAAGATAAACTCATTACCAAGCATCGTATTTTGCCTATTTTTAAACATGGCAATTTATTATTCGT
TGCGATCAGTAACCCCACCAATATTGAAGCAGTGGATGCCATTCGATTTGGTAGTAAGCTGAATACTGAAACAATTATTG
TTGAACATGACAAACTTGAAAAGTTAATTGAACAGAACTTTGCTGAAGAAAGTACTTTTGACTTTGATGATGACTTTGAT
CTTGATTTAGAACAGGAAACTATTGATCCAGCGAAAGAAGATGAAGATGAGGCAACGGGGGATGAAGCTCCGATTGTTAA
ATACATCAATAAGCTGCTAGTCGACGCAATTCGTATGGGTGCATCTGACTTACATTTTGAACCCTACGAAAAAACCTATC
GCGTCCGCTACCGTGTCGATGGCGTGTTAAGAACAATTACAAATCCGCCCTTGCAGCTCGCTACACGACTCGCTTCACGT
TTAAAAGTCATGTCACAAATGGACATCTCGGAAAAGCGTATACCTCAAGACGGTCGTATCAAGTTAAAGCTGTCAAAGAA
CAAAGCTATCGACTTCCGTGTGAACTCTTTACCGACATTGTTTGGTGAAAAACTTGTTCTACGTATCCTTGATCCATCTA
GTGCGATGCTAGGGATTGATGCGTTGGGTTATGAACCGGATCAAAAAGAACTGTTCATGCAGGCTTTAGAAAAACCACAA
GGCATGCTACTCATTACCGGTCCAACGGGTTCTGGTAAAACCGTATCTCTATATACAGGTTTAAACATTCTTAACCGTGA
AGATACCAACATTTCAACGGCCGAAGACCCTGTGGAAATTAACTTAGAAGGGATCAATCAGGTCAACGTAAATAATAAAG
TCGGCCTCACATTCTCTGCAGCCTTAAAATCATTCTTACGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGAGAT
TTAGAAACGGCTGAAATTGCCATTAAAGCAGCTCAAACAGGTCACATGGTGATGTCTACACTGCATACCAATAGTGCACC
TGAAACACTGACACGCTTACGCAATATGGGCGTGCCTTCATTTAACATCGCGACATCCGTGAACCTTGTCATTGCGCAGC
GACTTGCACGTCGCCTATGTTCACAATGTAAAGAAACAGTAGACGTACCAAAACAAAGTCTATTGGAGCTTGGTTTTACC
GAAGATGATTTAAATACACCAGAATTCCAAATATACCAGCCTGTGGGTTGTGCCGAATGCCGTGAAGGCTATAAAGGTCG
TGTGGGTATTTACGAAGTCATGAAAGTGACACCTGAAATTTCAAGAATTATTATGGAAGACGGCAATGCCTTACAAATTG
CCGAAGCTTCCAATCAAGCAGGTTTTAACAATTTACGCCGTTCAGGCTTAATTAAGGTCATGCAGGGGGTGACTTCACTT
CAAGAAGTCAATCGTGTGACCAGTGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.21

100

0.805
  pilB Acinetobacter baylyi ADP1

74.912

100

0.75
  pilB Legionella pneumophila strain ERS1305867

54.034

93.673

0.506
  pilF Neisseria gonorrhoeae MS11

49.198

98.594

0.485
  pilB Vibrio cholerae strain A1552

49.275

97.012

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

47.217

97.891

0.462
  pilB Vibrio campbellii strain DS40M4

46.679

97.891

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

34.244

100

0.374
  pilF Thermus thermophilus HB27

37.004

97.364

0.36

Multiple sequence alignment