Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G3M63_RS00075 Genome accession   NZ_CP048629
Coordinates   19414..21114 (+) Length   566 a.a.
NCBI ID   WP_163981513.1    Uniprot ID   A0A6C0TLD4
Organism   Pseudomonas sp. OIL-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 14414..26114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M63_RS00055 (G3M63_00055) - 15382..16173 (-) 792 WP_256669887.1 O-antigen ligase -
  G3M63_RS00060 (G3M63_00060) - 16199..17763 (+) 1565 WP_163984141.1 IS3 family transposase -
  G3M63_RS00065 (G3M63_00065) - 17708..18517 (-) 810 WP_163981511.1 hypothetical protein -
  G3M63_RS00070 (G3M63_00070) pilA 18612..19052 (-) 441 WP_163981512.1 pilin Machinery gene
  G3M63_RS00075 (G3M63_00075) pilB 19414..21114 (+) 1701 WP_163981513.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G3M63_RS00080 (G3M63_00080) pilC 21118..22383 (+) 1266 WP_150299529.1 type II secretion system F family protein Machinery gene
  G3M63_RS00085 (G3M63_00085) pilD 22387..23259 (+) 873 WP_163981514.1 A24 family peptidase Machinery gene
  G3M63_RS00090 (G3M63_00090) coaE 23261..23866 (+) 606 WP_163981515.1 dephospho-CoA kinase -
  G3M63_RS00095 (G3M63_00095) yacG 23863..24054 (+) 192 WP_150303146.1 DNA gyrase inhibitor YacG -
  G3M63_RS00100 (G3M63_00100) - 24072..25016 (+) 945 WP_163981516.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62451.69 Da        Isoelectric Point: 5.3381

>NTDB_id=421956 G3M63_RS00075 WP_163981513.1 19414..21114(+) (pilB) [Pseudomonas sp. OIL-1]
MDTPALSGLARRIVQDQLLDATVASKASKQATLDKIPLITYLVQNKLASSRDLAMACADEFGYPFFELGSLDKDHQPQEL
VSEKLIRQHCVLPLYKRGSRLFLAISDPTNQQALSDIQFNTGLMTDAVMVEDDKLHAAIDKYLESPTGGLGNMDDSSLDD
LEVESVNEDDKATGTGDADDAPIVRFVNKMLLDAVRMGSSDLHFEPYEKMYRVRFRTDGILHEIAKPPVQLGVRIAARLK
VMSSMDMAERRKPQDGRIKMKISRNKAIDFRVNTLPTLWGEKVVLRILDAESAKMGIDALGYEEDQKEMYLSALAKPQGM
ILVTGPTGSGKTVSLYTGLNILNTMERNISTAEDPVEINLEGINQVNVNPKQGLDFAKALRAFLRQDPDIIMVGEIRDLE
TAEIAIKAAQTGHMVLSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARRLCKTCKTPFEVPRETLLEEGFSPA
KIDAGLTIYGPVGCENCKDGYKGRVGIYEVVKITPAMQRIIMEDGNSIQISDVAQQEGFRSLRQSALMKAEQGVTSLAEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=421956 G3M63_RS00075 WP_163981513.1 19414..21114(+) (pilB) [Pseudomonas sp. OIL-1]
ATGGATACCCCCGCGCTCTCTGGTCTGGCACGAAGGATCGTTCAGGATCAGTTGCTGGACGCAACCGTTGCCAGCAAGGC
CAGCAAACAGGCCACGTTAGACAAGATTCCCCTCATTACCTATCTGGTACAGAACAAGCTGGCCAGTTCACGCGACCTGG
CGATGGCCTGTGCTGACGAATTCGGCTATCCCTTTTTTGAGTTGGGGTCACTGGACAAGGACCATCAGCCGCAGGAGCTG
GTCAGCGAAAAGCTGATCCGCCAGCATTGCGTATTGCCGCTGTATAAGCGCGGCTCGCGGCTGTTTCTGGCGATATCGGA
TCCGACCAATCAGCAGGCGCTGAGCGATATCCAGTTCAATACCGGTCTGATGACTGATGCGGTCATGGTCGAGGACGACA
AGCTCCATGCCGCCATCGACAAGTATCTGGAAAGCCCCACCGGTGGGCTCGGCAACATGGACGATTCAAGTCTGGATGAC
CTGGAAGTCGAGTCGGTCAATGAAGATGACAAGGCGACCGGTACCGGCGATGCGGACGACGCGCCTATCGTGCGCTTCGT
CAACAAGATGCTGCTGGATGCAGTGCGCATGGGCTCATCGGACTTGCATTTCGAACCCTATGAAAAAATGTACCGCGTGC
GCTTTCGTACCGACGGCATTCTGCATGAGATAGCCAAACCGCCAGTGCAGCTTGGTGTGCGCATCGCGGCACGCCTGAAA
GTCATGTCATCCATGGATATGGCCGAGCGGCGCAAGCCGCAGGATGGCCGCATCAAGATGAAAATCTCGCGGAACAAGGC
CATCGATTTTCGGGTAAATACCCTGCCCACGCTCTGGGGCGAGAAGGTGGTGCTGCGTATCCTCGACGCTGAGAGCGCCA
AAATGGGCATCGACGCGCTGGGCTATGAAGAAGACCAGAAGGAAATGTACCTCTCCGCACTGGCGAAGCCCCAGGGCATG
ATTCTGGTAACCGGGCCCACTGGTTCGGGCAAGACGGTGTCGCTGTATACCGGCCTGAACATCCTCAACACCATGGAACG
CAATATCTCCACCGCGGAAGATCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTGAACCCCAAGCAGGGGC
TGGATTTTGCCAAGGCGCTACGTGCGTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGGGAGATCCGGGACCTGGAG
ACCGCCGAGATCGCGATCAAGGCGGCGCAAACCGGTCACATGGTGCTTTCCACGCTGCACACCAATAGCGCGGCCGAAAC
CCTGACGCGTTTGCGCAACATGGGCGTGCCCTCGTTCAACATTGCAACTTCCGTCAATCTGATCATCGCCCAGCGTCTGG
CGCGTCGGCTCTGTAAAACCTGTAAGACGCCCTTCGAGGTGCCGCGCGAGACGCTGCTCGAAGAAGGCTTCAGCCCCGCC
AAAATTGATGCAGGGTTGACGATTTACGGGCCAGTAGGTTGCGAAAACTGTAAAGACGGTTATAAAGGGCGTGTTGGTAT
TTATGAAGTGGTAAAGATTACGCCAGCCATGCAACGCATCATCATGGAAGACGGCAACTCTATTCAGATTTCAGACGTAG
CCCAACAGGAAGGTTTCAGAAGCTTGCGCCAGTCGGCCCTGATGAAAGCAGAACAGGGTGTTACCAGTCTGGCGGAAGTT
AACCGAGTGACAAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6C0TLD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.011

100

0.59
  pilB Acinetobacter baylyi ADP1

59.115

99.823

0.59
  pilB Legionella pneumophila strain ERS1305867

57.067

100

0.571
  pilB Vibrio cholerae strain A1552

50.977

99.47

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.089

99.117

0.496
  pilF Neisseria gonorrhoeae MS11

48.592

100

0.488
  pilB Vibrio campbellii strain DS40M4

48.415

100

0.486
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.684

92.933

0.378
  pilF Thermus thermophilus HB27

39.245

93.64

0.367

Multiple sequence alignment