Detailed information NTG0042003

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: SPPN_RS06445
• Genome accession: NC_015875
• Coordinates: 1248776..1249426 (-)
• Length: 216 a.a.
• NCBI ID: WP_000387332.1
• Uniprot ID: A0A6I3U9C7
• Organism: Streptococcus pseudopneumoniae IS7493
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1243776..1254426

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPPN_RS06430	-	1244872..1245613 (+)	742	Protein_1304	FAD:protein FMN transferase	-
SPPN_RS12865	-	1245605..1245709 (-)	105	Protein_1305	IS5/IS1182 family transposase	-
SPPN_RS12110	-	1245815..1246500 (-)	686	Protein_1306	transposase family protein	-
SPPN_RS06440 (SPPN_06535)	comEC/celB	1246558..1248792 (-)	2235	WP_000942433.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SPPN_RS06445 (SPPN_06540)	comEA/celA/cilE	1248776..1249426 (-)	651	WP_000387332.1	helix-hairpin-helix domain-containing protein	Machinery gene
SPPN_RS06450 (SPPN_06545)	-	1249494..1250063 (-)	570	WP_000443735.1	GNAT family N-acetyltransferase	-
SPPN_RS06455 (SPPN_06550)	-	1250276..1251280 (-)	1005	WP_000658205.1	PhoH family protein	-
SPPN_RS06460 (SPPN_06555)	-	1251365..1251580 (-)	216	WP_001232084.1	YozE family protein	-
SPPN_RS06465 (SPPN_06560)	cvfB	1251589..1252443 (-)	855	WP_001095471.1	RNA-binding virulence regulatory protein CvfB	-
SPPN_RS06470 (SPPN_06565)	frr	1252503..1253060 (-)	558	WP_001262225.1	ribosome recycling factor	-
SPPN_RS06475 (SPPN_06570)	pyrH	1253069..1253806 (-)	738	WP_000002996.1	UMP kinase	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23219.57 Da
• Isoelectric Point: 5.5171

>NTDB_id=42003 SPPN_RS06445 WP_000387332.1 1248776..1249426(-) (comEA/celA/cilE) [Streptococcus pseudopneumoniae IS7493]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSIDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=42003 SPPN_RS06445 WP_000387332.1 1248776..1249426(-) (comEA/celA/cilE) [Streptococcus pseudopneumoniae IS7493]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAGCCAGCTCCACAAACACCTGTAAAGGAAACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAGAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAACAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAGCTTAAACAGGTTAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATAGACCATCGTGAGGCAAATGG
GAAATTCAAGTCGATTGATGAACTCAAGAAGGTCTCTGGCATTGGTGCTAAGACCATAGAAAAGCTAAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A6I3U9C7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae D39	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae R6	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	97.222	100	0.972
comEA/celA/cilE	Streptococcus mitis NCTC 12261	96.759	100	0.968
comEA/celA/cilE	Streptococcus mitis SK321	91.204	100	0.912
comEA	Lactococcus lactis subsp. cremoris KW2	44.053	100	0.463
comEA	Latilactobacillus sakei subsp. sakei 23K	34.335	100	0.37
comEA	Bacillus subtilis subsp. subtilis str. 168	42.105	87.963	0.37