Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IBL27_RS00970 Genome accession   NZ_CP061071
Coordinates   202964..203905 (+) Length   313 a.a.
NCBI ID   WP_002263435.1    Uniprot ID   Q8DS49
Organism   Streptococcus mutans B04Sm5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 197964..208905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IBL27_RS00960 (IBL27_00960) rpoC 198692..202321 (+) 3630 WP_002263433.1 DNA-directed RNA polymerase subunit beta' -
  IBL27_RS00965 (IBL27_00965) - 202449..202832 (+) 384 WP_002263434.1 DUF1033 family protein -
  IBL27_RS00970 (IBL27_00970) comGA 202964..203905 (+) 942 WP_002263435.1 competence type IV pilus ATPase ComGA Machinery gene
  IBL27_RS00975 (IBL27_00975) comGB 203841..204872 (+) 1032 WP_255264529.1 competence type IV pilus assembly protein ComGB Machinery gene
  IBL27_RS00980 (IBL27_00980) comGC 204872..205186 (+) 315 WP_002279331.1 competence type IV pilus major pilin ComGC Machinery gene
  IBL27_RS00985 (IBL27_00985) comGD 205173..205577 (+) 405 WP_002279330.1 competence type IV pilus minor pilin ComGD Machinery gene
  IBL27_RS00990 (IBL27_00990) comGE 205549..205842 (+) 294 WP_002263439.1 competence type IV pilus minor pilin ComGE Machinery gene
  IBL27_RS00995 (IBL27_00995) comGF 205829..206263 (+) 435 WP_002264245.1 competence type IV pilus minor pilin ComGF Machinery gene
  IBL27_RS01000 (IBL27_01000) comGG 206241..206630 (+) 390 WP_002263441.1 competence type IV pilus minor pilin ComGG Machinery gene
  IBL27_RS01005 (IBL27_01005) comGH 206685..207638 (+) 954 WP_002309243.1 class I SAM-dependent methyltransferase Machinery gene
  IBL27_RS01010 (IBL27_01010) - 207697..208896 (+) 1200 WP_002265895.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35739.09 Da        Isoelectric Point: 8.0347

>NTDB_id=419744 IBL27_RS00970 WP_002263435.1 202964..203905(+) (comGA) [Streptococcus mutans B04Sm5]
MVQKLGRKIIAEAVQNKAQDIYIIPKKESYELYMRIGNERRFIDVYDHHFMANLISHFKFVAGMNVGEKRRSQLGSCDYS
YDEGKVISLRLSSVGDYRGYESLVIRLLFSGRHDLKFWFNSMDLILNEIKGRGLYLFSGPVGSGKTSLMYQLVKLRFETK
QVITIEDPVEIKQDNMLQLQLNESIDMTYDNLIKLSLRHRPDILIIGEIRDKETARAVIRASLTGAMVFSTIHAKSIPGV
YARMLELGTSSEELQNALRGIVYQRLIGGGGIIDFAKGDYQNHSAKKWNKQIYDLFTAGYITEGEAQAEEIED

Nucleotide


Download         Length: 942 bp        

>NTDB_id=419744 IBL27_RS00970 WP_002263435.1 202964..203905(+) (comGA) [Streptococcus mutans B04Sm5]
ATGGTTCAAAAATTAGGTAGAAAAATCATTGCCGAAGCTGTTCAAAATAAAGCACAAGATATCTATATTATTCCTAAAAA
AGAATCTTATGAGCTTTATATGAGAATAGGGAATGAAAGGAGGTTCATTGATGTTTATGACCATCATTTCATGGCTAATC
TTATTAGTCACTTTAAATTTGTTGCAGGAATGAATGTTGGTGAGAAAAGACGAAGTCAGCTGGGCTCTTGTGATTATTCT
TATGATGAAGGGAAAGTTATTTCATTAAGGTTATCAAGTGTAGGAGATTATCGTGGTTATGAAAGTTTGGTTATTCGCTT
ACTTTTTTCTGGACGTCACGATTTAAAATTCTGGTTTAACAGTATGGATTTGATTTTAAATGAAATCAAGGGGCGAGGGC
TTTATCTTTTTTCAGGTCCAGTTGGAAGTGGCAAAACAAGTTTGATGTATCAATTGGTAAAACTAAGATTTGAGACCAAA
CAGGTAATTACCATTGAAGATCCTGTGGAAATTAAGCAAGATAATATGTTACAACTGCAGCTTAATGAAAGCATTGATAT
GACTTATGATAATTTAATTAAATTATCTCTGAGGCATCGTCCGGATATTTTAATTATTGGAGAAATTCGAGATAAAGAAA
CCGCCAGAGCTGTTATTAGAGCAAGTCTTACAGGGGCAATGGTATTCTCAACTATCCATGCTAAAAGCATTCCAGGTGTT
TATGCTAGGATGTTAGAATTGGGTACTAGTTCAGAAGAATTACAAAATGCTTTGCGTGGTATTGTTTATCAACGTTTAAT
AGGGGGAGGAGGGATTATTGACTTTGCCAAAGGGGATTATCAAAACCACTCAGCAAAAAAGTGGAACAAACAAATTTATG
ACCTTTTTACAGCAGGATATATCACTGAAGGAGAAGCACAAGCAGAAGAAATTGAAGATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DS49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Streptococcus mutans UA159

100

100

1
  comGA Streptococcus mutans UA140

99.681

100

0.997
  comGA Streptococcus gordonii str. Challis substr. CH1

67.093

100

0.671
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

66.026

99.681

0.658
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

65.916

99.361

0.655
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

65.916

99.361

0.655
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.916

99.361

0.655
  comGA/cglA/cilD Streptococcus pneumoniae D39

65.916

99.361

0.655
  comGA/cglA/cilD Streptococcus pneumoniae R6

65.916

99.361

0.655
  comGA Lactococcus lactis subsp. cremoris KW2

52.412

99.361

0.521