Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GVU25_RS01435 Genome accession   NZ_CP048014
Coordinates   316558..318270 (-) Length   570 a.a.
NCBI ID   WP_004975865.1    Uniprot ID   -
Organism   Acinetobacter towneri strain 205     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 311558..323270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVU25_RS01405 (GVU25_01400) - 311592..311915 (+) 324 WP_004975854.1 pyrimidine/purine nucleoside phosphorylase -
  GVU25_RS01410 (GVU25_01405) rlmB 312104..312853 (+) 750 WP_004975856.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GVU25_RS01415 (GVU25_01410) - 312948..313826 (+) 879 WP_042068576.1 DMT family transporter -
  GVU25_RS01420 (GVU25_01415) coaE 313832..314431 (-) 600 WP_004975860.1 dephospho-CoA kinase -
  GVU25_RS13845 (GVU25_01420) pilD 314441..315301 (-) 861 WP_096901344.1 prepilin peptidase Machinery gene
  GVU25_RS01430 (GVU25_01425) pilC 315301..316527 (-) 1227 WP_004975864.1 type II secretion system F family protein Machinery gene
  GVU25_RS01435 (GVU25_01430) pilB 316558..318270 (-) 1713 WP_004975865.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GVU25_RS01440 (GVU25_01435) tpiA 318548..319339 (+) 792 WP_004975867.1 triose-phosphate isomerase -
  GVU25_RS01445 (GVU25_01440) secG 319353..319682 (+) 330 WP_004975868.1 preprotein translocase subunit SecG -
  GVU25_RS01470 (GVU25_01465) rimP 320634..321158 (+) 525 WP_004975871.1 ribosome maturation factor RimP -
  GVU25_RS01475 (GVU25_01470) nusA 321194..322678 (+) 1485 WP_131261098.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63472.67 Da        Isoelectric Point: 4.8148

>NTDB_id=419429 GVU25_RS01435 WP_004975865.1 316558..318270(-) (pilB) [Acinetobacter towneri strain 205]
MSVQQATPRFTGFIRRLVEDGLVSAENMQTAIKAAQKDNVDIVPYLIQNLNIAPLIIAEKISNEFGEPIFDLSVYDSALI
VREGIDEKLFSKYCILPIFKRNHLLYVATSNPTNIEAMDAIRFNSKMNIEAIVVEHDKLVKLIEQNFASESAFEFEDDDF
DLNFEDGNSEPEQAAEDEPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKNKSIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKAPSDIPKQSLLEMGF
TEADLSNPEFQVYQPVGCSECREGYKGRVGVYEVMKVTPEISRIIMEDGNAIQIADASAKSGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=419429 GVU25_RS01435 WP_004975865.1 316558..318270(-) (pilB) [Acinetobacter towneri strain 205]
ATGTCAGTACAACAAGCTACACCTAGATTTACAGGTTTTATTCGACGTTTGGTCGAAGATGGGCTCGTTTCTGCTGAAAA
TATGCAAACTGCCATTAAAGCCGCACAAAAAGATAATGTTGATATTGTTCCCTATCTCATTCAAAACCTAAATATCGCGC
CTTTAATCATTGCTGAAAAAATTTCTAATGAGTTTGGCGAACCTATTTTTGATTTAAGCGTGTATGACTCTGCGCTCATC
GTGCGCGAAGGCATTGATGAAAAACTGTTCAGCAAATATTGCATATTGCCTATTTTTAAACGCAATCATCTGCTTTATGT
TGCAACCAGTAACCCGACCAATATCGAAGCAATGGATGCCATTCGTTTCAACAGTAAAATGAATATTGAAGCCATTGTGG
TCGAACACGATAAATTGGTAAAACTGATTGAACAAAACTTTGCGAGTGAGTCTGCTTTTGAATTTGAAGATGATGACTTC
GATCTCAACTTTGAAGATGGTAACAGCGAACCAGAACAAGCTGCAGAAGATGAACCGCAGGGTGATGAATCCCCAATTGT
TAAATACATCAACAAATTACTGATTGATGCAATTCGCATGGGTGCTTCGGACTTACACTTTGAACCGTATGAAAAAACCT
ATCGAGTGCGTTACCGTGTCGATGGTGTATTACGTCAAATTGCCACCCCACCCTTACAGTTAGCTAACCGCTTGGCATCA
CGTTTAAAAGTGATGTCCCAAATGGACATTTCTGAAAAGCGTGTCCCGCAAGATGGTCGTATTAAATTAAAACTATCTAA
AAATAAATCCATCGACTTCCGTGTGAACTCGTTGCCAACATTGTTCGGTGAAAAAATCGTACTGCGTATTCTTGACCCAT
CTAGCGCGATGCTTGGGATTGACGCTTTGGGCTATGAACCAGAACAAAAAGAATTGTTCATGGAAGCCTTAGAAAAACCG
CAAGGCATGTTACTGATTACAGGTCCAACGGGTTCGGGTAAAACGGTATCGCTATATACAGGCTTAAATATCTTAAACAG
AGAAGATACCAACATTTCCACTGCCGAAGACCCTGTGGAAATTAACTTAGAAGGCATCAACCAGGTTAACGTCAACAATA
AAGTGGGCTTAACCTTCGCTGCCGCTCTGAAATCTTTCTTACGTCAAGACCCTGATATTGTCATGGTGGGTGAGATTCGT
GACCTTGAAACTGCAGAAATTGCAATTAAAGCAGCCCAAACAGGTCACATGGTTTTATCTACCCTCCACACCAACAGTGC
GCCAGAAACTTTAACGCGTTTACGCAATATGGGCGTTCCTTCCTTTAACATCGCAACTTCGGTAAACCTCGTCATTGCAC
AGCGTTTGGCACGACGCCTATGCCCGCATTGCAAAGCTCCTTCCGATATTCCAAAACAAAGCTTATTGGAAATGGGCTTT
ACTGAAGCCGATTTGAGCAATCCAGAATTTCAGGTCTATCAACCTGTAGGCTGTTCTGAATGCCGTGAAGGTTATAAAGG
ACGTGTTGGTGTTTATGAAGTGATGAAAGTCACACCAGAAATTTCTAGAATTATTATGGAGGATGGTAATGCCATCCAAA
TTGCTGATGCGTCAGCAAAATCGGGGTTTAACAATCTGCGTCGTTCGGGGTTACTTAAGGTCATGCAGGGGGTGACTTCA
TTGCAAGAAGTCAATCGTGTGACCAGTGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.246

100

0.782
  pilB Acinetobacter baylyi ADP1

76.316

100

0.763
  pilB Legionella pneumophila strain ERS1305867

50.345

100

0.512
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.587

99.298

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

48.214

98.246

0.474
  pilB Vibrio campbellii strain DS40M4

50.482

91.053

0.46
  pilF Thermus thermophilus HB27

37.435

100

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

34.951

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.544

98.596

0.37

Multiple sequence alignment