Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GVU25_RS13845 Genome accession   NZ_CP048014
Coordinates   314441..315301 (-) Length   286 a.a.
NCBI ID   WP_096901344.1    Uniprot ID   A0A2A5PJ16
Organism   Acinetobacter towneri strain 205     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 309441..320301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVU25_RS01405 (GVU25_01400) - 311592..311915 (+) 324 WP_004975854.1 pyrimidine/purine nucleoside phosphorylase -
  GVU25_RS01410 (GVU25_01405) rlmB 312104..312853 (+) 750 WP_004975856.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GVU25_RS01415 (GVU25_01410) - 312948..313826 (+) 879 WP_042068576.1 DMT family transporter -
  GVU25_RS01420 (GVU25_01415) coaE 313832..314431 (-) 600 WP_004975860.1 dephospho-CoA kinase -
  GVU25_RS13845 (GVU25_01420) pilD 314441..315301 (-) 861 WP_096901344.1 prepilin peptidase Machinery gene
  GVU25_RS01430 (GVU25_01425) pilC 315301..316527 (-) 1227 WP_004975864.1 type II secretion system F family protein Machinery gene
  GVU25_RS01435 (GVU25_01430) pilB 316558..318270 (-) 1713 WP_004975865.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GVU25_RS01440 (GVU25_01435) tpiA 318548..319339 (+) 792 WP_004975867.1 triose-phosphate isomerase -
  GVU25_RS01445 (GVU25_01440) secG 319353..319682 (+) 330 WP_004975868.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32011.38 Da        Isoelectric Point: 8.0564

>NTDB_id=419458 GVU25_RS13845 WP_096901344.1 314441..315301(-) (pilD) [Acinetobacter towneri strain 205]
MQELIAYFIQNPTALYIAVGLFSLCIGSFLNVVIYRTPKMMEQEWRNDCQMLLHPEQAIIDESKITLSKPASTCPKCHNP
IRWYQNIPVLSWLVLRGKCGNCQNLISVRYPMIEVLTAVCALIVLAVFGPTVQMLFGLLLTYTLIALTFIDFDTQLLPDR
YTLPLAALGLAVNSYAIYTSPTSAIWGYVIGFLCLWVVYYLFKIVTGKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSL
VGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=419458 GVU25_RS13845 WP_096901344.1 314441..315301(-) (pilD) [Acinetobacter towneri strain 205]
ATGCAAGAATTAATCGCGTACTTTATCCAAAATCCAACTGCGCTCTATATCGCAGTTGGACTTTTTAGTTTATGTATTGG
CAGTTTTTTGAATGTGGTGATTTACCGCACCCCAAAAATGATGGAACAGGAGTGGCGTAATGACTGCCAAATGTTATTAC
ATCCTGAACAAGCCATTATTGATGAAAGTAAAATCACCCTGAGCAAACCTGCATCAACCTGCCCCAAATGTCACAACCCA
ATTCGTTGGTATCAAAATATTCCTGTGCTGAGTTGGTTGGTATTACGCGGTAAATGTGGCAACTGCCAAAACCTGATCAG
CGTACGTTATCCAATGATTGAAGTATTAACCGCTGTTTGCGCCCTGATTGTGCTTGCGGTATTTGGCCCAACTGTACAAA
TGCTGTTTGGTTTGCTGCTCACTTATACCTTGATTGCACTCACCTTCATTGATTTCGATACACAACTCTTGCCAGACCGT
TATACCCTGCCTTTGGCTGCACTGGGTTTGGCCGTCAACAGCTATGCCATCTATACCTCACCAACCTCGGCAATTTGGGG
CTATGTGATTGGCTTCCTATGTCTATGGGTGGTGTATTACCTGTTTAAAATCGTCACAGGCAAAGAAGGCATGGGCTATG
GTGACTTCAAGCTCCTTGCCGCTTTAGGTGCATGGATGGGGCCGATGATGTTACCCCTGATTGTGCTGCTATCGTCCTTA
GTTGGGGCGATTATTGGCATTATTCTGTTAAAAGTACGTAAAGAAAACCAACCCTTTGCTTTTGGTCCATATATTGCCAT
TGCGGGCTGGATTGCCTTTTTATGGGGCGAGCAAATCATGAAAGTATATTTGGGACAATAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A5PJ16

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

80.702

99.65

0.804
  pilD Acinetobacter baumannii D1279779

80.351

99.65

0.801
  pilD Vibrio cholerae strain A1552

54.851

93.706

0.514
  pilD Vibrio campbellii strain DS40M4

52.788

94.056

0.497
  pilD Neisseria gonorrhoeae MS11

46.154

95.455

0.441

Multiple sequence alignment