Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GWG05_RS02170 Genome accession   NZ_CP047982
Coordinates   439691..441397 (-) Length   568 a.a.
NCBI ID   WP_042077628.1    Uniprot ID   -
Organism   Aeromonas caviae strain 1507-17068     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 434691..446397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWG05_RS02140 (GWG05_02145) mutT 435311..435715 (+) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -
  GWG05_RS02145 (GWG05_02150) yacG 435771..435965 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  GWG05_RS02150 (GWG05_02155) zapD 435976..436698 (-) 723 WP_043154103.1 cell division protein ZapD -
  GWG05_RS02155 (GWG05_02160) coaE 436736..437350 (-) 615 WP_049637196.1 dephospho-CoA kinase -
  GWG05_RS02160 (GWG05_02165) pilD 437369..438244 (-) 876 WP_053288093.1 prepilin peptidase Machinery gene
  GWG05_RS02165 (GWG05_02170) pilC 438322..439563 (-) 1242 WP_180907144.1 type II secretion system F family protein Machinery gene
  GWG05_RS02170 (GWG05_02175) pilB 439691..441397 (-) 1707 WP_042077628.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GWG05_RS02175 (GWG05_02180) - 441401..441841 (-) 441 WP_180907305.1 pilin -
  GWG05_RS02180 (GWG05_02185) nadC 442166..443029 (-) 864 WP_180907145.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GWG05_RS02185 (GWG05_02190) - 443033..443503 (-) 471 WP_180907146.1 TIGR02281 family clan AA aspartic protease -
  GWG05_RS02190 (GWG05_02195) ampD 443616..444185 (+) 570 WP_180907147.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GWG05_RS02195 (GWG05_02200) pdhR 444464..445228 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62633.01 Da        Isoelectric Point: 5.8214

>NTDB_id=418967 GWG05_RS02170 WP_042077628.1 439691..441397(-) (pilB) [Aeromonas caviae strain 1507-17068]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDENSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKGQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTNEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=418967 GWG05_RS02170 WP_042077628.1 439691..441397(-) (pilB) [Aeromonas caviae strain 1507-17068]
ATGACCTCTAGCCTTAATAGCGGCCTAGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCAAGGGCCCAGCGCAAGCCCTTTGTCACCTTTCTGATTGAGAACGACATCCTCGACAGCAAGGCCCTCG
CCGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTGGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACACCACGTCCTGCCCATCTATACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAACGTGTCTGCCCTGGAGGATTTCGGCTTCAGCTTCGGTCTGCACACGGAAGCCTTGCTGGTCGAGGAGAGCA
AGCTGACCGCCGCCATCGGCAAATTGATGGAAAGCGAGCAGGATACCCTCGGCATGGATCACATCGACGAGGCCGAGATC
TCCGAGCTCGAGGTGTCCGACGAGAACTCTCGTCTCGATGAGAGCGTCAATACGGCCGACGATGACGCCCCCATCGTCAA
GTACATCCACAAGATCATGATGGACGCCATCAAGCGCGGCGCATCGGACCTGCATTTCGAACCTTACGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTGAACCTGGCAAACCGTTTCGCGGCACGC
TTAAAAGTCATGGCGCGGCTCGACATCGCCGAGCGGCGTCTGCCCCAGGATGGCCGCATCAAGCTCAAGATTTCGCGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTCATCCGACTTCTGGATTCATCTG
CAGCCAGGCTCAATATCGATCAACTCGGCTTCGACGACCGCCAGAAGGGGCAATACCTGCATGCCCTCTCCAAACCCCAG
GGGATGATACTGGTCACCGGCCCCACGGGGTCGGGCAAGACGGTATCGCTCTATACCGGTCTCAACATCCTCAATACCAA
CGAGGTCAACATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTGAACCAGGTGCAGATCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTGTCCACCCTGCACACCAATTCGGCGGC
CGAGACGCTCACCCGGATGATGAACATGGGTGTCCCCGCCTTCAACATCGCCTCCTCGGTCACCCTCATCATGGCCCAGC
GCCTCGCCCGCAAGTTGTGCGATCACTGCAAGGCGCCCGAGGTGGTACCGGAAGCCGAGCTGCTGGAGCTGGGCTTTACC
CAGCAGCAACTGGCCGCCGGCTTGCGACTGTTCAAACCCGTGGGGTGCAAGGAGTGCTCGGGAGGCTACCGAGGGCGGGT
CGGGATCTATGAGATCATGCTGATGTCAGACAACATCGCCAAGCTCATCATGCAAGGAGCCAACTCGCTGCAAATCGCGG
CCATCGCCCAGAAGGAAGGGATGCGCACCCTGCGCACCTCCGGCCTTGAAAAAGCCCGCCTGGGAGTCACCAGCCTGGCC
GAAATAAACAGGATCACTACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.885

99.12

0.544
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

53.24

100

0.535
  pilB Vibrio campbellii strain DS40M4

50.53

99.648

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.12

0.495
  pilF Neisseria gonorrhoeae MS11

46.208

99.824

0.461
  pilF Thermus thermophilus HB27

40.341

92.958

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.681

97.183

0.366

Multiple sequence alignment