Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FX986_RS13085 Genome accession   NZ_CP047970
Coordinates   3113669..3115405 (-) Length   578 a.a.
NCBI ID   WP_191236640.1    Uniprot ID   -
Organism   Cobetia marina strain GPM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3108669..3120405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX986_RS13060 - 3109260..3110213 (+) 954 WP_077371708.1 Nudix family hydrolase -
  FX986_RS13065 yacG 3110342..3110587 (-) 246 WP_191236637.1 DNA gyrase inhibitor YacG -
  FX986_RS13070 coaE 3110580..3111227 (-) 648 WP_191236638.1 dephospho-CoA kinase -
  FX986_RS13075 pilD 3111227..3112087 (-) 861 WP_254696420.1 prepilin peptidase Machinery gene
  FX986_RS13080 pilC 3112186..3113424 (-) 1239 WP_191236639.1 type II secretion system F family protein Machinery gene
  FX986_RS13085 pilB 3113669..3115405 (-) 1737 WP_191236640.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FX986_RS13090 - 3116167..3116646 (+) 480 WP_191236641.1 pilin -
  FX986_RS13095 - 3116694..3116996 (-) 303 WP_191236642.1 hypothetical protein -
  FX986_RS13100 - 3117121..3118401 (-) 1281 WP_191236643.1 cation diffusion facilitator family transporter -
  FX986_RS13105 - 3118480..3120039 (-) 1560 WP_254696421.1 PhnE/PtxC family ABC transporter permease -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63516.56 Da        Isoelectric Point: 4.8035

>NTDB_id=418852 FX986_RS13085 WP_191236640.1 3113669..3115405(-) (pilB) [Cobetia marina strain GPM2]
MPQTDQTLKGFAARLVESGLLEPQAALLAEQEANEAGESLLLHVITHGLASAREATLVAAWEYGLPYVDLDAIRIDSLPS
IDELPEKVLRKLGVLPLYRREHRLTVGVPSPSTLTHLDELQFATGLSIESVLVAADQLAPALEQYLEAGEESAMDMLSED
GEALDGLEFEGEEDNNHDMAITANSDDAPVVRFVHKVLLDAIKRGASDIHFEPYESTYRIRFRIDGMLVENTRPPFNMRN
RISARLKVMSRMDISERRLPQDGAIKLRLSKSRSIDFRVNTLPTVYGEKLVLRILDPSSAKMGIEALGFEPEQRAMFEKT
LGEPQGMILVTGPTGSGKTVTLYTGLNILNTPERNISTAEDPVELKVEGVNQVNVLPKIGLDFASALRAFLRQDPDVVMV
GEIRDKETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLSNMGIAPFNIASSVSLIIAQRLARRLCPHCRQPADIPQEAMA
SMGFSAENGFSAEDLENATIYRPTGCSQCTLGYKGRVGIYEVMPISDEMSRLIMREGTAIELADQARKEGLSDLRRSGLR
KVMAGMTSLEEVNRITKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=418852 FX986_RS13085 WP_191236640.1 3113669..3115405(-) (pilB) [Cobetia marina strain GPM2]
ATGCCCCAGACTGATCAGACGTTAAAGGGATTTGCCGCTCGCCTGGTGGAATCAGGCTTGCTGGAGCCTCAGGCTGCCTT
ACTGGCTGAGCAGGAGGCCAATGAGGCTGGCGAAAGTCTGCTGCTGCATGTCATCACTCATGGCCTGGCTTCGGCACGTG
AGGCTACCTTGGTGGCAGCATGGGAATACGGGCTGCCCTATGTGGATCTCGATGCAATACGTATCGATTCACTCCCGAGC
ATTGATGAGCTGCCAGAGAAGGTACTGCGAAAGCTGGGCGTACTGCCGCTCTATAGACGCGAACATCGTTTGACCGTCGG
CGTGCCCTCGCCATCGACACTGACTCACCTTGATGAATTGCAGTTTGCCACCGGCCTGTCGATTGAAAGCGTGCTGGTTG
CAGCCGATCAACTCGCACCTGCGCTTGAGCAATATCTGGAAGCCGGCGAAGAAAGCGCCATGGACATGCTGAGTGAAGAT
GGTGAGGCTCTGGATGGGCTCGAATTCGAAGGCGAAGAAGACAACAACCATGATATGGCCATCACAGCCAATAGTGATGA
TGCGCCAGTCGTGCGCTTCGTTCACAAGGTACTGTTGGATGCCATCAAACGTGGCGCCTCGGATATCCACTTTGAACCCT
ATGAATCCACGTACCGGATTCGCTTTCGTATCGATGGCATGCTGGTCGAGAATACTCGTCCGCCCTTCAACATGCGCAAC
CGTATCTCGGCGCGCTTGAAGGTGATGTCGCGCATGGATATTTCCGAGCGACGTCTGCCTCAAGATGGCGCCATCAAATT
GCGCCTGTCCAAGTCACGCTCCATCGATTTTCGTGTCAACACCTTGCCAACCGTTTACGGGGAGAAGTTGGTACTGCGTA
TTCTTGACCCCAGTTCCGCCAAGATGGGCATCGAGGCGCTGGGCTTCGAGCCCGAACAGCGTGCCATGTTCGAGAAGACA
CTTGGCGAGCCTCAGGGCATGATCCTTGTCACCGGCCCTACCGGTAGCGGCAAGACCGTTACCCTGTATACCGGCTTGAA
CATCCTCAACACGCCTGAGCGCAATATCTCGACCGCGGAAGACCCGGTCGAGCTCAAAGTGGAGGGTGTCAATCAGGTCA
ACGTACTGCCCAAGATAGGGCTCGACTTTGCCTCGGCACTGCGCGCCTTCCTGCGCCAGGATCCCGACGTGGTGATGGTC
GGGGAGATTCGCGACAAGGAAACCGCGGAAATCGCCGTCAAGGCCGCGCAGACAGGCCACCTGGTGCTCTCGACCCTGCA
TACCAATTCTGCCCCTGAAACGTTGACGCGCCTCAGCAACATGGGGATCGCGCCCTTCAACATCGCAAGCTCGGTTTCCT
TGATCATCGCGCAACGTCTGGCACGGCGTCTGTGTCCCCACTGTCGCCAACCTGCCGATATCCCCCAGGAAGCCATGGCC
TCAATGGGCTTCAGCGCAGAAAATGGCTTCAGTGCGGAAGATCTGGAAAATGCCACCATCTATCGTCCGACGGGTTGCAG
CCAATGCACGCTAGGGTACAAGGGACGCGTCGGCATCTATGAAGTGATGCCCATCTCTGATGAGATGAGCCGCTTGATCA
TGCGGGAAGGCACCGCGATAGAACTGGCGGACCAGGCGCGCAAGGAAGGATTGTCGGATCTGCGCCGCAGCGGCCTACGC
AAGGTCATGGCGGGCATGACCTCGCTGGAAGAGGTCAACCGTATCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.972

98.962

0.524
  pilB Acinetobacter baumannii D1279779

52.159

100

0.522
  pilB Acinetobacter baylyi ADP1

51.736

99.654

0.516
  pilB Vibrio campbellii strain DS40M4

47.652

99.481

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

48.063

98.27

0.472
  pilB Vibrio cholerae strain A1552

49.094

95.502

0.469
  pilF Neisseria gonorrhoeae MS11

48.381

96.194

0.465
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.046

97.924

0.382
  pilB/pilB1 Synechocystis sp. PCC 6803

41.874

90.484

0.379
  pilF Thermus thermophilus HB27

38.053

97.751

0.372

Multiple sequence alignment