Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FX986_RS13075 Genome accession   NZ_CP047970
Coordinates   3111227..3112087 (-) Length   286 a.a.
NCBI ID   WP_254696420.1    Uniprot ID   -
Organism   Cobetia marina strain GPM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3106227..3117087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX986_RS13055 argJ 3107928..3109151 (+) 1224 WP_084209208.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  FX986_RS13060 - 3109260..3110213 (+) 954 WP_077371708.1 Nudix family hydrolase -
  FX986_RS13065 yacG 3110342..3110587 (-) 246 WP_191236637.1 DNA gyrase inhibitor YacG -
  FX986_RS13070 coaE 3110580..3111227 (-) 648 WP_191236638.1 dephospho-CoA kinase -
  FX986_RS13075 pilD 3111227..3112087 (-) 861 WP_254696420.1 prepilin peptidase Machinery gene
  FX986_RS13080 pilC 3112186..3113424 (-) 1239 WP_191236639.1 type II secretion system F family protein Machinery gene
  FX986_RS13085 pilB 3113669..3115405 (-) 1737 WP_191236640.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FX986_RS13090 - 3116167..3116646 (+) 480 WP_191236641.1 pilin -
  FX986_RS13095 - 3116694..3116996 (-) 303 WP_191236642.1 hypothetical protein -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31050.33 Da        Isoelectric Point: 8.2191

>NTDB_id=418850 FX986_RS13075 WP_254696420.1 3111227..3112087(-) (pilD) [Cobetia marina strain GPM2]
MDSLALLSPALQACLAGIFGLLIGSFLNVVITRLPVMMMRQWRAEAREALELEPEASPRFNLLVPASRCPGCDTPIKWHD
NLPIIGWLKRRGRCASCDTRISAQYPLVELAGGALGASIALIYGLEAQSLWIFGACLTLLVLSVIDFRTQLLPDAITLPL
LWAGLAYQLLFQPLMLPSAVIGAMAGYLVLWSFYWLFKLVTGKEGMGYGDFKLLAALGAWLGWQWLPLLLILSAGLGAVL
GILLQLLVPRLRGAPMPFGPYLAVAGWIALLFGTPLMSLYLSVIGA

Nucleotide


Download         Length: 861 bp        

>NTDB_id=418850 FX986_RS13075 WP_254696420.1 3111227..3112087(-) (pilD) [Cobetia marina strain GPM2]
GTGGATTCTCTCGCCTTACTCTCCCCTGCTCTCCAGGCCTGCCTTGCGGGGATTTTCGGCCTGTTGATCGGCAGTTTTCT
GAATGTAGTGATCACACGCCTGCCGGTGATGATGATGCGTCAGTGGCGTGCAGAGGCACGTGAAGCGCTGGAACTGGAGC
CGGAAGCGTCGCCACGCTTCAATCTGTTGGTGCCTGCCTCACGCTGCCCTGGCTGCGACACCCCAATCAAATGGCATGAC
AATCTCCCTATCATCGGCTGGCTGAAACGCCGCGGCCGCTGCGCGAGCTGCGATACCCGTATCAGTGCACAGTATCCACT
GGTGGAGCTCGCCGGTGGCGCGCTCGGCGCTTCCATCGCGTTGATCTACGGCCTGGAAGCACAGAGCCTGTGGATCTTCG
GCGCCTGTCTGACACTGCTGGTGTTGTCAGTGATCGATTTCCGCACCCAGCTGTTGCCTGATGCCATCACGCTACCGTTG
CTGTGGGCCGGTCTCGCCTACCAACTGCTTTTCCAGCCATTGATGCTGCCAAGTGCCGTGATTGGTGCCATGGCCGGCTA
TCTGGTGCTGTGGAGCTTCTACTGGCTGTTCAAGCTGGTGACTGGCAAGGAAGGCATGGGCTATGGCGATTTCAAGCTGT
TGGCGGCACTGGGCGCCTGGCTTGGCTGGCAGTGGTTGCCACTGCTGTTGATCCTCTCTGCGGGACTCGGCGCAGTACTC
GGCATCCTGTTGCAACTGCTGGTACCGCGCCTGCGGGGAGCCCCCATGCCATTCGGCCCTTATCTGGCCGTGGCAGGCTG
GATCGCACTGCTGTTCGGCACCCCGCTGATGAGCCTCTACCTCAGCGTGATAGGTGCCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-121)

Type IV leader peptidase family (Peptidase_A24)

(136-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

51.736

100

0.521
  pilD Vibrio campbellii strain DS40M4

47.5

97.902

0.465
  pilD Neisseria gonorrhoeae MS11

47.778

94.406

0.451
  pilD Acinetobacter baumannii D1279779

48.134

93.706

0.451
  pilD Acinetobacter nosocomialis M2

47.761

93.706

0.448

Multiple sequence alignment