Detailed information NTG0418322

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GV831_RS00740
• Genome accession: NZ_CP047891
• Coordinates: 146871..147518 (+)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis subsp. lactis strain WHH2311
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 141871..152518

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GV831_RS00715 (GV831_00700)	-	142000..143178 (+)	1179	WP_014570696.1	SLC13 family permease	-
GV831_RS00720 (GV831_00705)	-	143351..143914 (+)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
GV831_RS00725 (GV831_00710)	-	144018..144416 (+)	399	WP_004255236.1	hypothetical protein	-
GV831_RS00730 (GV831_00715)	-	144521..145453 (+)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
GV831_RS00735 (GV831_00720)	-	145450..146811 (+)	1362	WP_004255239.1	ABC transporter permease	-
GV831_RS00740 (GV831_00725)	comEA	146871..147518 (+)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
GV831_RS00745 (GV831_00730)	comEC	147499..149709 (+)	2211	WP_014570695.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GV831_RS00750 (GV831_00735)	-	149994..150770 (+)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
GV831_RS00755 (GV831_00740)	-	150954..151169 (+)	216	WP_014570694.1	F0F1 ATP synthase subunit C	-
GV831_RS00760 (GV831_00745)	atpB	151214..151927 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
GV831_RS00765 (GV831_00750)	atpF	151942..152448 (+)	507	WP_004255258.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=418322 GV831_RS00740 WP_004255240.1 146871..147518(+) (comEA) [Lactococcus lactis subsp. lactis strain WHH2311]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=418322 GV831_RS00740 WP_004255240.1 146871..147518(+) (comEA) [Lactococcus lactis subsp. lactis strain WHH2311]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367