Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   M594_RS06495 Genome accession   NZ_CP047883
Coordinates   1319066..1319713 (-) Length   215 a.a.
NCBI ID   WP_173876288.1    Uniprot ID   A0A6M9FB73
Organism   Streptococcus mitis strain S022-V3-A4     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1314066..1324713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M594_RS06480 (M594_06500) - 1314668..1315852 (-) 1185 WP_173876285.1 hypothetical protein -
  M594_RS06485 (M594_06505) - 1315856..1316443 (-) 588 WP_173876286.1 ATP-binding cassette domain-containing protein -
  M594_RS10130 - 1316476..1316700 (-) 225 WP_254597133.1 hypothetical protein -
  M594_RS06490 (M594_06510) comEC/celB 1316842..1319082 (-) 2241 WP_173876287.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  M594_RS06495 (M594_06515) comEA/celA/cilE 1319066..1319713 (-) 648 WP_173876288.1 helix-hairpin-helix domain-containing protein Machinery gene
  M594_RS06500 (M594_06520) - 1319780..1320349 (-) 570 WP_153209672.1 GNAT family N-acetyltransferase -
  M594_RS06505 (M594_06525) ald 1320521..1321633 (+) 1113 WP_173876289.1 alanine dehydrogenase -
  M594_RS06510 (M594_06530) - 1321690..1322685 (-) 996 WP_125447093.1 PhoH family protein -
  M594_RS06515 (M594_06535) - 1322770..1322985 (-) 216 WP_049488667.1 YozE family protein -
  M594_RS06520 (M594_06540) - 1323031..1323588 (-) 558 WP_173876290.1 GrpB family protein -
  M594_RS06525 (M594_06545) cvfB 1323600..1324454 (-) 855 WP_173876291.1 RNA-binding virulence regulatory protein CvfB -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 23279.61 Da        Isoelectric Point: 5.5117

>NTDB_id=418263 M594_RS06495 WP_173876288.1 1319066..1319713(-) (comEA/celA/cilE) [Streptococcus mitis strain S022-V3-A4]
MEAIIEKIKEYKIIVICTCLGFLVGGFFLLKPAPQTPVKETNLQAEVVAVSKDSVSEKEVTKEEKEELVEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSINLAQKVSDEALVYVPTKGEEANQQTNSGVSSSTSKDKKVNLNKAS
MEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide


Download         Length: 648 bp        

>NTDB_id=418263 M594_RS06495 WP_173876288.1 1319066..1319713(-) (comEA/celA/cilE) [Streptococcus mitis strain S022-V3-A4]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTACAAAATCATTGTCATTTGTACTTGTCTGGGCTTTCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAGGAAACGAATTTGCAGGCAGAAGTTGTAGCAGTTTCCAAGGATT
CGGTGTCCGAAAAGGAAGTGACCAAGGAAGAAAAGGAAGAACTGGTTGAACAAGATCTAATCACAGTAGATGTGAAGGGT
GCTGTCAAATCGCCAGGAATTTATGACTTGCCAGTAGGTAGTCGGGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGATCAATCTAGCTCAGAAAGTCAGTGATGAGGCTCTGGTTTACGTTCCAACTAAGGGAG
AAGAAGCTAATCAACAGACTAATTCGGGGGTGTCTTCTTCAACAAGCAAGGACAAGAAGGTCAATCTCAACAAAGCCAGT
ATGGAAGAACTCAAGCAGGTCAAAGGACTGGGTGGCAAACGAGCTCAGGATATTATCGACCATCGTGAGGCAAATGGGAA
ATTCAAGTCGGTTGATGAACTTAAGAAGGTCTCTGGCATTGGTGCTAAAACGATAGAAAAGCTAAAAGACTATGTTACAG
TGGATTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(76-126)

Helix-hairpin-helix motif (HHH_3)

(151-213)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M9FB73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus mitis NCTC 12261

93.056

100

0.935
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

92.13

100

0.926
  comEA/celA/cilE Streptococcus pneumoniae D39

91.667

100

0.921
  comEA/celA/cilE Streptococcus pneumoniae R6

91.667

100

0.921
  comEA/celA/cilE Streptococcus pneumoniae Rx1

91.667

100

0.921
  comEA/celA/cilE Streptococcus mitis SK321

90.741

100

0.912
  comEA Lactococcus lactis subsp. cremoris KW2

40.889

100

0.428
  comEA Bacillus subtilis subsp. subtilis str. 168

38.725

94.884

0.367

Multiple sequence alignment