Detailed information NTG0414622

Bioinformatically predicted

Overview

• Name: comGA/cglA/cilD
• Type: Machinery gene
• Locus tag: GU337_RS11315
• Genome accession: NZ_CP047614
• Coordinates: 2227960..2228901 (-)
• Length: 313 a.a.
• NCBI ID: WP_061774317.1
• Uniprot ID: -
• Organism: Lactococcus raffinolactis strain Lr_19_7
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	2229337..2230335	2227960..2228901	flank	436

Gene organization within MGE regions

Location: 2227960..2230335

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GU337_RS11315 (GU337_11255)	comGA/cglA/cilD	2227960..2228901 (-)	942	WP_061774317.1	competence type IV pilus ATPase ComGA	Machinery gene
GU337_RS11320 (GU337_11260)	-	2228964..2229251 (-)	288	WP_167840317.1	hypothetical protein	-
GU337_RS11325 (GU337_11265)	-	2229337..2230335 (+)	999	WP_024047037.1	IS5-like element IS1194 family transposase	-

Sequence

Protein

• Length: 313 a.a.
• Molecular weight: 34933.21 Da
• Isoelectric Point: 9.1747

>NTDB_id=414622 GU337_RS11315 WP_061774317.1 2227960..2228901(-) (comGA/cglA/cilD) [Lactococcus raffinolactis strain Lr_19_7]
MIKEIAQRMLKKASDFGASDIYILPARTGFSVVFRKSAHREYDQLLSDAEGQSLISHFKFTAGMNVGEKRRPQLGSCLYE
LADRKCRLRLSSAGDFESRESLVIRILHDTKQPLKFWIEADLPQVKKLVARRGLYLFAGPVGSGKTTLMHHIAKEKFSGQ
QVITIEDPVEIVAPDLLQFQLNEAIGNTYDSLIKLSLRHMPDLVIVGEIRDQETARAVMRASLTGYTVFSTIHAKSIAGV
YARLLELGVTKEEINNSLSGVVYQRLIAGKGVLDSAEKAFERHANDKWHAKIEKLVTEGHLTPDQATAEKVSD

Nucleotide

• Length: 942 bp

>NTDB_id=414622 GU337_RS11315 WP_061774317.1 2227960..2228901(-) (comGA/cglA/cilD) [Lactococcus raffinolactis strain Lr_19_7]
ATGATAAAGGAAATAGCGCAAAGGATGTTAAAGAAAGCAAGCGATTTTGGGGCAAGTGATATTTATATATTACCCGCCAG
AACAGGATTTTCGGTTGTTTTCAGAAAGTCAGCACATCGTGAATATGATCAGCTCCTGTCAGATGCTGAAGGTCAAAGTC
TCATTTCCCATTTCAAATTTACAGCAGGGATGAATGTAGGTGAGAAGCGCCGGCCACAACTAGGATCTTGCTTGTATGAG
CTTGCAGATAGAAAGTGTCGCTTGCGTCTATCCTCAGCGGGTGACTTTGAAAGTCGCGAAAGTTTGGTGATTCGAATTTT
GCATGACACGAAACAACCACTCAAGTTTTGGATTGAGGCAGATTTACCTCAGGTAAAAAAGTTAGTAGCTAGACGCGGTC
TTTATTTATTTGCGGGGCCAGTTGGTTCTGGGAAAACGACGCTGATGCATCACATTGCCAAAGAAAAGTTTTCTGGTCAG
CAAGTCATTACGATTGAAGATCCAGTTGAAATCGTTGCACCAGATCTGCTCCAATTCCAACTGAATGAAGCGATTGGCAA
CACCTATGATAGCTTGATTAAATTATCCTTGCGCCATATGCCAGATCTTGTGATTGTCGGAGAAATTCGAGATCAGGAAA
CTGCGCGAGCAGTCATGAGGGCCAGTCTGACGGGCTATACTGTTTTTTCGACGATTCATGCTAAGTCGATTGCGGGTGTT
TATGCGCGATTATTGGAGCTTGGCGTAACAAAAGAAGAAATCAATAATTCGTTGTCAGGTGTTGTCTATCAACGGTTGAT
TGCAGGAAAGGGGGTGCTAGATAGTGCTGAAAAAGCATTCGAAAGGCATGCCAACGACAAGTGGCATGCCAAAATTGAAA
AATTGGTTACAGAAGGACATCTCACACCTGATCAGGCGACAGCCGAAAAGGTTAGCGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-267)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	57.692	99.681	0.575
comGA/cglA/cilD	Streptococcus pneumoniae R6	56.731	99.681	0.565
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	56.731	99.681	0.565
comGA/cglA/cilD	Streptococcus pneumoniae D39	56.731	99.681	0.565
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	56.731	99.681	0.565
comGA	Lactococcus lactis subsp. cremoris KW2	56.23	100	0.562
comYA	Streptococcus gordonii str. Challis substr. CH1	55.911	100	0.559
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	53.674	100	0.537
comYA	Streptococcus mutans UA140	52.548	100	0.527
comYA	Streptococcus mutans UA159	52.548	100	0.527