Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GM320_RS06070 Genome accession   NZ_CP047422
Coordinates   1189284..1190552 (+) Length   422 a.a.
NCBI ID   WP_159352539.1    Uniprot ID   -
Organism   Shewanella algae strain 18064-CSB-B-B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1184284..1195552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM320_RS06050 (GM320_06050) ampD 1184501..1185079 (-) 579 WP_156020396.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GM320_RS06055 (GM320_06055) nadC 1185238..1186098 (+) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GM320_RS06060 (GM320_06060) - 1186967..1187407 (+) 441 WP_159352537.1 pilin -
  GM320_RS06065 (GM320_06065) pilB 1187422..1189128 (+) 1707 WP_159352538.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GM320_RS06070 (GM320_06070) pilC 1189284..1190552 (+) 1269 WP_159352539.1 type II secretion system F family protein Machinery gene
  GM320_RS06075 (GM320_06075) pilD 1190629..1191543 (+) 915 WP_144128769.1 prepilin peptidase Machinery gene
  GM320_RS06080 (GM320_06080) coaE 1191544..1192155 (+) 612 WP_025012056.1 dephospho-CoA kinase -
  GM320_RS06085 (GM320_06085) zapD 1192152..1192892 (+) 741 WP_028779562.1 cell division protein ZapD -
  GM320_RS06090 (GM320_06090) yacG 1192937..1193149 (+) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  GM320_RS06095 (GM320_06095) mutT 1193149..1193547 (+) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45902.07 Da        Isoelectric Point: 10.2016

>NTDB_id=413447 GM320_RS06070 WP_159352539.1 1189284..1190552(+) (pilC) [Shewanella algae strain 18064-CSB-B-B]
MATATATAKSNKAKNNGAKPQPKIHTFTWKGLNRDGKQSSGELRGTSIAEIKSLLKSQGINPKLVRKKSASLFGGDKKIK
PMDIAVITRQIATMLAAGVPLVTSIELIARGHEKNKVRVLLGTVLADVQAGIPLSDALRPHRVYFDDLYVDLVAAGEHSG
SLDAVFDRIATYREKAEALKSKIKKAMFYPAAVVVVAIAVTTLLLLFVVPQFEEIFSSFGAELPAFTQLIVNISRGLQST
WYLFLAAIVISVWLFVRAHRNSEHFRNRIDEMVLKIPLIGEILHKAAMARFARTLATTFAAGVPLIDGLESAAGASGNFV
YRKALLNVRTEVMAGMQMNVAMRTTKLFPDMLIQMVMIGEESGSLDNMLNKIANIYEMQVDDAVDGLSSLIEPIMMVVIG
TLVGGLIVGMYLPIFQMGNVVG

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=413447 GM320_RS06070 WP_159352539.1 1189284..1190552(+) (pilC) [Shewanella algae strain 18064-CSB-B-B]
ATGGCAACTGCAACGGCGACAGCAAAAAGCAATAAAGCCAAAAATAATGGCGCCAAGCCCCAGCCCAAGATCCATACCTT
TACCTGGAAAGGCCTCAATCGTGACGGCAAGCAAAGCTCCGGCGAACTGCGTGGTACTAGTATAGCCGAAATCAAGTCCC
TGCTTAAGAGCCAGGGGATCAACCCTAAACTGGTTCGCAAGAAATCTGCTTCCCTTTTCGGTGGTGATAAAAAAATCAAA
CCTATGGATATCGCGGTGATTACTCGTCAGATAGCCACTATGTTGGCTGCCGGCGTACCACTGGTTACTTCCATCGAATT
GATCGCTCGCGGTCACGAAAAAAACAAGGTGCGGGTTCTGCTTGGCACTGTGTTGGCCGATGTCCAGGCCGGCATTCCAC
TCTCCGATGCCCTCCGCCCACACAGAGTCTATTTTGACGACCTCTATGTCGACTTGGTGGCCGCCGGTGAACATTCAGGC
TCTTTGGATGCCGTATTCGACCGCATTGCTACCTATAGGGAAAAGGCCGAAGCCCTTAAGTCAAAAATCAAGAAAGCCAT
GTTCTACCCCGCAGCAGTAGTCGTCGTTGCCATTGCAGTAACCACTTTACTATTGCTCTTTGTAGTACCCCAGTTTGAAG
AAATTTTCAGTAGCTTTGGCGCCGAACTGCCTGCCTTTACTCAGTTAATTGTCAATATCAGCCGCGGCCTTCAGAGTACC
TGGTATCTATTTCTAGCAGCCATTGTAATCAGTGTTTGGCTGTTTGTACGCGCTCACCGCAACTCAGAGCACTTCCGTAA
CCGTATCGACGAAATGGTGCTGAAGATCCCACTTATTGGTGAAATTCTCCATAAAGCTGCCATGGCCCGTTTTGCCCGCA
CTCTGGCCACCACTTTTGCCGCCGGGGTGCCACTGATTGATGGCCTGGAGTCTGCCGCCGGCGCCTCCGGTAATTTTGTT
TACCGCAAGGCGCTGTTGAATGTGCGCACCGAAGTGATGGCCGGGATGCAGATGAACGTGGCCATGCGCACCACCAAGCT
ATTCCCTGATATGCTGATCCAGATGGTGATGATAGGTGAAGAATCCGGCTCATTGGACAACATGCTCAACAAGATTGCCA
ATATCTACGAGATGCAGGTGGATGATGCCGTAGACGGTCTCTCCAGCCTGATAGAACCCATTATGATGGTAGTTATCGGC
ACCCTGGTTGGTGGCCTTATCGTCGGCATGTACCTGCCTATCTTCCAGATGGGTAACGTGGTAGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(88-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.14

94.55

0.531
  pilC Acinetobacter baylyi ADP1

50.123

96.445

0.483
  pilC Legionella pneumophila strain ERS1305867

49.01

95.735

0.469
  pilC Acinetobacter baumannii D1279779

48.995

94.313

0.462
  pilC Vibrio cholerae strain A1552

45.885

95.024

0.436
  pilC Vibrio campbellii strain DS40M4

44.665

95.498

0.427
  pilG Neisseria meningitidis 44/76-A

41.687

98.341

0.41
  pilG Neisseria gonorrhoeae MS11

41.687

98.341

0.41

Multiple sequence alignment