Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GM320_RS06065 Genome accession   NZ_CP047422
Coordinates   1187422..1189128 (+) Length   568 a.a.
NCBI ID   WP_159352538.1    Uniprot ID   -
Organism   Shewanella algae strain 18064-CSB-B-B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1182422..1194128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM320_RS06040 (GM320_06040) pdhR 1182568..1183320 (-) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GM320_RS06045 (GM320_06045) ampE 1183596..1184447 (-) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  GM320_RS06050 (GM320_06050) ampD 1184501..1185079 (-) 579 WP_156020396.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GM320_RS06055 (GM320_06055) nadC 1185238..1186098 (+) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GM320_RS06060 (GM320_06060) - 1186967..1187407 (+) 441 WP_159352537.1 pilin -
  GM320_RS06065 (GM320_06065) pilB 1187422..1189128 (+) 1707 WP_159352538.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GM320_RS06070 (GM320_06070) pilC 1189284..1190552 (+) 1269 WP_159352539.1 type II secretion system F family protein Machinery gene
  GM320_RS06075 (GM320_06075) pilD 1190629..1191543 (+) 915 WP_144128769.1 prepilin peptidase Machinery gene
  GM320_RS06080 (GM320_06080) coaE 1191544..1192155 (+) 612 WP_025012056.1 dephospho-CoA kinase -
  GM320_RS06085 (GM320_06085) zapD 1192152..1192892 (+) 741 WP_028779562.1 cell division protein ZapD -
  GM320_RS06090 (GM320_06090) yacG 1192937..1193149 (+) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  GM320_RS06095 (GM320_06095) mutT 1193149..1193547 (+) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63032.17 Da        Isoelectric Point: 5.1753

>NTDB_id=413446 GM320_RS06065 WP_159352538.1 1187422..1189128(+) (pilB) [Shewanella algae strain 18064-CSB-B-B]
MPTTGLHLGLSTLFIRKGLLDESSLAEAIKQSQKNKQALVTTLVTSQLLSARSIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLDGLDEES
LAGIEVTDTDKRQQEENEGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEEIPEHELLQLGFT
QQQIDAGFTPYKPCGCEFCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATTAKQQGMRDLRLSGLLKVIQGVTSIA
EVNRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=413446 GM320_RS06065 WP_159352538.1 1187422..1189128(+) (pilB) [Shewanella algae strain 18064-CSB-B-B]
ATGCCTACTACCGGCCTCCATTTAGGATTATCGACCCTATTTATCCGTAAGGGGCTGCTCGATGAGAGTAGCCTGGCAGA
GGCCATTAAGCAGTCGCAAAAAAACAAACAAGCTCTGGTCACCACTCTGGTGACCAGTCAACTTTTATCTGCCCGCAGCA
TAGCCGAACTCTGTTATGAGGAGTATGGCACCCCGCTGCTGGATCTTAATGAGTTTGATTTAAGCAGTATCCCGGAAGAG
TTTCTCAATAAAAAGCTGATCGATAAGCACAGATGTCTGCCGCTATTTAAACGTGGCAATCGCCTCTATATCGCCACCTC
AGACCCCACCAATATTGCCGCTCTGGAAGATTTTCAGTTTAGCGCCGGCCTGCATGCAGAAGCGATACTGGTAGAGGAAG
ATAAGCTCAGTAAAGCACTGGAAAAAGTGCTTGAAGAGGACTTGAGTTCATTGGATCTGGATGGTCTCGATGAAGAGTCC
CTCGCCGGTATCGAGGTCACAGATACCGACAAGCGCCAACAGGAAGAGAATGAAGGCAAAGACGATGCGCCTATCGTCAT
CTACATCAATAAGATTTTGACCGATGCCATCCGCAAGGGCGCATCGGATCTGCATTTCGAACCCTACGAGAAACGCTACC
GCATCCGTTTTCGTATCGATGGCATCCTCCACGAGGTGTCCGAGCCACCGATTTCTCTCTCGGGCCGTATATCAGCCCGC
TTGAAAGTGATGTCCAAACTGGATATCGCCGAACGCCGGGTCCCTCAGGATGGCCGCATCAAGATGAAGCTGTCCCGCAA
CAAGAGTATCGACTTTCGGGTCAGTACCCTGCCCACCCTCTGGGGTGAAAAAATCGTGATGCGGATCCTCGATTCCTCTT
CCGCGCAATTAGGAATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTACCTGGAGATGCTGGCAAGGCCCCAG
GGGATGATTCTGGTGACAGGCCCTACCGGCTCTGGTAAAACCGTGTCCCTCTATACTGGCCTCAATATCCTCAATACCGA
GGAGCGCAATATCAGCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGGGTCAACCAGGTGCATATCAACCCCAAGG
CAGGGCTAACCTTCGCCTCGGCGCTGCGCTCCTTTTTACGTCAGGACCCGGATGTGGTAATGGTGGGCGAAATCCGCGAC
TTGGAAACAGCAGAAATCGCCATCAAGGCGGCACAAACCGGCCACTTGGTACTCTCAACCCTGCACACCAACTCATCGGC
CGAAACCCTTACTCGCCTGCTCAACATGGGGGTTCCCGGTTACAACATTGCCAGTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGCCAACCGGAAGAGATCCCCGAGCATGAACTGCTGCAGCTGGGCTTCACT
CAGCAGCAGATAGATGCAGGTTTTACCCCATACAAGCCCTGCGGCTGCGAATTCTGCTCCGGTGGCTACAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATCGCCAGAACTATTATGGAGGGCGGTAATTCACTGCAGATTGCCA
CTACTGCCAAACAGCAGGGGATGCGCGACCTGCGCCTTTCTGGCCTTTTAAAAGTGATTCAAGGCGTCACCAGTATTGCC
GAAGTCAATCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.147

99.12

0.586
  pilB Acinetobacter baylyi ADP1

59.18

98.768

0.585
  pilB Legionella pneumophila strain ERS1305867

56.36

99.648

0.562
  pilB Vibrio parahaemolyticus RIMD 2210633

53.086

99.824

0.53
  pilB Vibrio cholerae strain A1552

53.237

97.887

0.521
  pilB Vibrio campbellii strain DS40M4

51.687

99.12

0.512
  pilF Neisseria gonorrhoeae MS11

47.965

99.472

0.477
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.427

98.239

0.387
  pilF Thermus thermophilus HB27

37.876

99.472

0.377

Multiple sequence alignment