Detailed information NTG0411605

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GSF12_RS05130
• Genome accession: NZ_CP047226
• Coordinates: 1110501..1111664 (+)
• Length: 387 a.a.
• NCBI ID: WP_159374622.1
• Uniprot ID: A0A6P1KB66
• Organism: Moraxella osloensis strain YV1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1105501..1116664

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GSF12_RS05110 (GSF12_05110)	-	1106324..1106611 (+)	288	WP_159374618.1	pyrimidine/purine nucleoside phosphorylase	-
GSF12_RS05115 (GSF12_05115)	-	1106678..1107640 (-)	963	WP_201450496.1	pirin family protein	-
GSF12_RS05120 (GSF12_05120)	-	1107805..1109760 (-)	1956	WP_159374620.1	ATP-binding cassette domain-containing protein	-
GSF12_RS05125 (GSF12_05125)	-	1109865..1110140 (+)	276	WP_159374621.1	SlyX family protein	-
GSF12_RS05130 (GSF12_05130)	pilU	1110501..1111664 (+)	1164	WP_159374622.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GSF12_RS05135 (GSF12_05135)	-	1111786..1112883 (-)	1098	WP_159374623.1	3-deoxy-7-phosphoheptulonate synthase	-
GSF12_RS05140 (GSF12_05140)	lpxB	1113189..1114577 (+)	1389	WP_159374624.1	lipid-A-disaccharide synthase	-
GSF12_RS05145 (GSF12_05145)	-	1114648..1116078 (-)	1431	WP_159374625.1	ABC1 kinase family protein	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 43596.05 Da
• Isoelectric Point: 6.9785

>NTDB_id=411605 GSF12_RS05130 WP_159374622.1 1110501..1111664(+) (pilU) [Moraxella osloensis strain YV1]
MSHNESLISVAEETLHEAKTVMFNMLSKVVEIGGSDLFISADFPPSIKYHGLMKPLNKQPLTAEKTKLFAYSLMNQKQRE
EFATNLECNFAINVPNVSRFRVNVFQQQLQVGMVIRTITAEIPNFKQLLLPEQLKKIIMEKRGLVLVVGGTGSGKSTSLA
AMIDHRNENSAGHIITVEDPVEYVHKHKQSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMENAIAFAETG
HLCLGTLHANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTEDGKGRRAAVEIMLNTPLMADLILKSNFHE
IKALMTKSRELGMQTFDQALFDLYDEGAISYDEAIRNADSANELRLQIKLKSRRGEGDTQSTSELKL

Nucleotide

• Length: 1164 bp

>NTDB_id=411605 GSF12_RS05130 WP_159374622.1 1110501..1111664(+) (pilU) [Moraxella osloensis strain YV1]
ATGAGTCATAATGAATCTTTAATCAGTGTTGCCGAAGAAACGTTACATGAAGCCAAAACAGTCATGTTTAATATGCTGTC
CAAAGTGGTAGAAATCGGTGGTTCTGATTTGTTTATTTCAGCGGATTTTCCACCCAGCATTAAATATCACGGACTCATGA
AACCGCTCAATAAACAACCACTGACGGCTGAAAAAACCAAACTGTTTGCCTATAGTTTGATGAACCAAAAACAGCGCGAA
GAATTTGCAACCAATCTTGAGTGCAACTTTGCGATAAATGTGCCCAATGTATCGCGATTTCGGGTTAATGTGTTTCAGCA
GCAATTACAAGTGGGTATGGTGATTCGTACCATTACGGCAGAAATACCCAACTTTAAACAGTTGTTGCTACCTGAGCAGC
TCAAAAAAATTATCATGGAAAAACGTGGGTTAGTGTTGGTCGTAGGAGGAACGGGGTCAGGTAAATCAACATCGCTGGCG
GCAATGATTGACCATCGTAATGAAAACTCCGCAGGTCACATCATCACCGTCGAAGACCCAGTAGAATATGTGCACAAGCA
CAAACAATCCATGATCACCCACCGTGAAGTAGGTGTAGATAGTCATTCATGGCATAACGCACTCAAAAACACCTTGCGCC
AAGCACCTGATGTGATTTTGATTGGGGAAATTCGTGATACTGAAACTATGGAAAACGCCATTGCATTTGCTGAAACAGGT
CACTTATGTTTAGGCACTTTGCATGCCAATAATGCCAATCAAACCTTAGATCGTATTATCAACTTTTTTCCAGAGGAGCG
TCGTAACCAATTGCTGATGGACTTGTCATCGAATATGAAAGCTATCATTTCGCAGCGTTTGATTCGTACAGAAGATGGTA
AAGGTCGTCGGGCGGCGGTAGAAATTATGCTTAATACCCCATTGATGGCGGATTTGATATTAAAAAGTAATTTTCATGAA
ATTAAAGCACTGATGACCAAATCTCGGGAGTTGGGTATGCAAACCTTTGACCAAGCCTTATTTGATCTATACGATGAAGG
CGCCATTTCGTATGATGAAGCAATTCGCAATGCCGACTCTGCCAACGAACTGCGGTTGCAAATCAAACTTAAAAGCCGAC
GCGGTGAAGGCGACACGCAAAGCACAAGTGAGCTAAAGCTTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (124-290)

3D structure

• AlphaFold DB: A0A6P1KB66

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.057	89.922	0.54
pilU	Acinetobacter baylyi ADP1	56.897	89.922	0.512
pilU	Vibrio cholerae strain A1552	51.603	88.63	0.457
pilT	Acinetobacter baumannii strain A118	43.558	84.238	0.367
pilT	Acinetobacter nosocomialis M2	43.558	84.238	0.367
pilT	Acinetobacter baumannii D1279779	43.558	84.238	0.367
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.349	88.114	0.364