Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GST84_RS25125 Genome accession   NZ_CP047152
Coordinates   5448979..5450241 (-) Length   420 a.a.
NCBI ID   WP_135192480.1    Uniprot ID   -
Organism   Pseudomonas putida strain PP27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5443979..5455241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GST84_RS25110 (GST84_24760) - 5445663..5447246 (-) 1584 WP_021783679.1 SPOR domain-containing protein -
  GST84_RS25115 (GST84_24765) aroB 5447262..5448359 (-) 1098 WP_003249149.1 3-dehydroquinate synthase -
  GST84_RS25120 (GST84_24770) aroK 5448456..5448974 (-) 519 WP_010955625.1 shikimate kinase AroK -
  GST84_RS25125 (GST84_24775) pilQ 5448979..5450241 (-) 1263 WP_135192480.1 type IV pilus secretin PilQ Machinery gene
  GST84_RS25130 (GST84_24780) - 5450165..5451166 (-) 1002 WP_135192489.1 pilus assembly protein PilP -
  GST84_RS25135 (GST84_24785) - 5451178..5451714 (-) 537 WP_021783676.1 PilN domain-containing protein -
  GST84_RS25140 (GST84_24790) pilM 5451711..5452592 (-) 882 WP_101196374.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45786.47 Da        Isoelectric Point: 4.9919

>NTDB_id=411067 GST84_RS25125 WP_135192480.1 5448979..5450241(-) (pilQ) [Pseudomonas putida strain PP27]
MKTWMKYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQLLDAHLQPLRRELLPIHHAKAADLAQLLQASLEGDGIPTGSLSVDERT
NTLVVYLPAERLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=411067 GST84_RS25125 WP_135192480.1 5448979..5450241(-) (pilQ) [Pseudomonas putida strain PP27]
ATGAAAACGTGGATGAAATACCTGCTGGCAACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTTCAGGATGTAGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCCGGCGTCAACCTGGTCG
CCAGCGATGCAGTACAAGGCAGTGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCGCTTGACCTGGTGCTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGTAATGTGCTGTTGGTGGCGCCTGCAGCCGAGTTTGCCGCGCAATCAGCAGA
TGCCCGGGTGAGTCAGTTGCTGGATGCGCACCTGCAGCCATTGCGTCGGGAGCTGCTGCCCATTCACCACGCCAAGGCCG
CCGATTTGGCGCAGTTGCTGCAGGCAAGTCTGGAGGGTGACGGCATCCCCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACGCTGGTGGTGTATCTGCCCGCTGAACGCCTGGCCGAGTTGCGCCAGCTGGTGGCGCAGCTGGATGTGCCGGTGCG
CCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTATGAGAAAAGCCTGGGGGTGCGCTGGGGCGGGCCGC
TGTACGGCGAGAAGTTGCGGCCCGGCAAGGAACTGTTCGTCGACCTCGGCTTGGAGCGTGCCGGCAGCAGTATCGGCCTT
GGTTTGTTGCGCGGTGACGTATTGCTGGACCTGGAGCTCAGTGCCATGGAGAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACGAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACCCCGGACAACAAGGTG
ATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAATGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTGCGTGTGGCGGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

60.092

100

0.624
  comQ Acinetobacter baylyi ADP1

42.762

100

0.457
  pilQ Acinetobacter baumannii D1279779

41.432

100

0.455
  pilQ Acinetobacter baumannii strain A118

41.432

100

0.455
  pilQ Legionella pneumophila strain Lp02

43.914

99.762

0.438
  pilQ Legionella pneumophila strain ERS1305867

43.914

99.762

0.438
  pilQ Vibrio campbellii strain DS40M4

40.92

100

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.005

99.762

0.419
  pilQ Vibrio cholerae strain A1552

42.005

99.762

0.419

Multiple sequence alignment