Detailed information NTG0041070

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: SGPB_RS02990
• Genome accession: NC_015600
• Coordinates: 561458..562123 (+)
• Length: 221 a.a.
• NCBI ID: WP_013851600.1
• Uniprot ID: F5X5G1
• Organism: Streptococcus pasteurianus ATCC 43144
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 556458..567123

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SGPB_RS02960 (SGPB_0535)	-	556533..557711 (+)	1179	WP_013851598.1	MFS transporter	-
SGPB_RS02965 (SGPB_0536)	-	557766..558626 (+)	861	WP_003063816.1	helix-turn-helix transcriptional regulator	-
SGPB_RS02970 (SGPB_0537)	-	558631..559260 (+)	630	WP_013851599.1	ROK family protein	-
SGPB_RS02975 (SGPB_0538)	-	559492..559773 (-)	282	WP_003063819.1	GIY-YIG nuclease family protein	-
SGPB_RS02980 (SGPB_0539)	-	559748..560509 (-)	762	WP_003063822.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
SGPB_RS02985 (SGPB_0540)	-	560617..561363 (+)	747	WP_003063827.1	lysophospholipid acyltransferase family protein	-
SGPB_RS02990 (SGPB_0541)	comEA	561458..562123 (+)	666	WP_013851600.1	helix-hairpin-helix domain-containing protein	Machinery gene
SGPB_RS02995 (SGPB_0542)	comEC/celB	562116..564347 (+)	2232	WP_013851601.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SGPB_RS03000 (SGPB_0543)	holA	564420..565457 (+)	1038	WP_043910377.1	DNA polymerase III subunit delta	-
SGPB_RS03005 (SGPB_0544)	sodA	565546..566154 (+)	609	WP_013851603.1	superoxide dismutase SodA	-
SGPB_RS03010 (SGPB_0545)	-	566303..567028 (+)	726	WP_013851604.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Sequence

Protein

• Length: 221 a.a.
• Molecular weight: 23571.26 Da
• Isoelectric Point: 4.2822

>NTDB_id=41070 SGPB_RS02990 WP_013851600.1 561458..562123(+) (comEA) [Streptococcus pasteurianus ATCC 43144]
MIEEIKEKLLENKTLAAVLGIILVMIIGFFAWLNVTKTTADAQNDLPALSTTFSTSSSASDLKTERSSSQTETQKVFVDI
KGAVKNEGVYELSNGSRVTDVVKLAGGFTEDADKKSVNLAEKVTDESVIYVARVDENVAPATTNSQANGSAQQEESSDKI
NLNTATLAELQTISGIGAKRAQDIIDYRDANGGFSSVDDLANVSGIGEKTLEKLKSEVTVD

Nucleotide

• Length: 666 bp

>NTDB_id=41070 SGPB_RS02990 WP_013851600.1 561458..562123(+) (comEA) [Streptococcus pasteurianus ATCC 43144]
ATGATTGAAGAAATAAAAGAAAAACTTTTGGAGAACAAAACGTTGGCAGCGGTATTGGGGATAATACTTGTCATGATAAT
CGGATTTTTTGCTTGGTTAAATGTGACAAAAACAACAGCGGATGCGCAAAATGACCTACCTGCCCTAAGCACGACTTTTT
CGACAAGTAGTAGCGCAAGTGATTTAAAAACAGAACGTTCAAGTTCGCAAACTGAGACTCAAAAAGTGTTTGTAGATATT
AAAGGCGCTGTGAAAAATGAGGGGGTTTATGAGCTTTCAAACGGTAGCCGTGTGACAGATGTGGTCAAATTAGCAGGTGG
TTTTACAGAAGATGCTGATAAGAAGTCGGTGAATCTGGCTGAAAAAGTAACCGATGAATCGGTGATTTATGTGGCAAGAG
TTGATGAAAATGTTGCTCCTGCAACGACTAATTCACAAGCAAATGGCAGCGCTCAACAGGAAGAAAGTTCAGATAAAATC
AATCTTAATACCGCAACTTTGGCGGAACTCCAGACGATTTCTGGCATTGGCGCCAAACGTGCCCAAGATATTATTGACTA
CCGTGATGCTAATGGTGGTTTTTCATCAGTTGATGACTTGGCGAATGTGTCTGGTATCGGAGAAAAAACACTAGAAAAGC
TAAAATCTGAGGTGACCGTTGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (157-219)

• SLBB domain (SLBB) (78-131)

3D structure

• AlphaFold DB: F5X5G1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	47.699	100	0.516
comEA/celA/cilE	Streptococcus mitis SK321	46.154	100	0.462
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	44.545	99.548	0.443
comEA/celA/cilE	Streptococcus mitis NCTC 12261	43.891	100	0.439
comEA/celA/cilE	Streptococcus pneumoniae Rx1	43.636	99.548	0.434
comEA/celA/cilE	Streptococcus pneumoniae D39	43.636	99.548	0.434
comEA/celA/cilE	Streptococcus pneumoniae R6	43.636	99.548	0.434
comEA	Latilactobacillus sakei subsp. sakei 23K	37.946	100	0.385