Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GQR90_RS03715 Genome accession   NZ_CP047025
Coordinates   856761..858497 (-) Length   578 a.a.
NCBI ID   WP_158772935.1    Uniprot ID   -
Organism   Cobetia sp. L2A1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 851761..863497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQR90_RS03690 - 852325..853278 (+) 954 WP_158772931.1 Nudix family hydrolase -
  GQR90_RS03695 yacG 853379..853624 (-) 246 WP_158772932.1 DNA gyrase inhibitor YacG -
  GQR90_RS03700 coaE 853697..854329 (-) 633 WP_158772933.1 dephospho-CoA kinase -
  GQR90_RS03705 pilD 854326..855123 (-) 798 WP_158775288.1 A24 family peptidase Machinery gene
  GQR90_RS03710 pilC 855284..856522 (-) 1239 WP_158772934.1 type II secretion system F family protein Machinery gene
  GQR90_RS03715 pilB 856761..858497 (-) 1737 WP_158772935.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GQR90_RS03720 - 859096..859560 (+) 465 WP_158772936.1 pilin -
  GQR90_RS03725 - 859640..859942 (-) 303 WP_158772937.1 hypothetical protein -
  GQR90_RS03730 - 860005..861297 (-) 1293 WP_158772938.1 cation diffusion facilitator family transporter -
  GQR90_RS03735 - 861421..862980 (-) 1560 WP_233266418.1 ABC transporter permease subunit -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63510.57 Da        Isoelectric Point: 4.9444

>NTDB_id=409960 GQR90_RS03715 WP_158772935.1 856761..858497(-) (pilB) [Cobetia sp. L2A1]
MPQTNHTLKGFAASLVEAGLLEAQAAKLAEQEANEAGESLLLHVITHGLAPAREATLAAAWEYGLPYVDIDALRIDSLPS
IDELPEKVLRKLGVLPLYRREHRLTVGVPSPSTLTHLDELQFATGLSIESVLVAADQLTPALEQYLEAGEESAMDMLGEE
GEALDNLEFENEEDKSNDHAITANSDDAPVVRFVHKVLLDAIKRGASDIHFEPYESTYRIRFRIDGMLIENTRPPFNMRN
RISARLKVMSRMDIAERRLPQDGAIKLRISKSRSIDFRVNSLPTVYGEKLVLRILDPSSAKMGIEALGFEPEQRAMFEKT
LDEPQGMILVTGPTGSGKTVTLYTGLNILNTAERNISTAEDPVELKVEGVNQVNVLPKIGLDFASALRAFLRQDPDVVMV
GEIRDKETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLSNMGIAPFNIASSVSLIIAQRLARRLCTHCRQPAEIPPEALV
SMGFSTENGFSAEELESATIHRPVGCSQCTLGYKGRVGIYEVMPISDEMSRLIMREGSAIELADQARKEGLSDLRRSGLR
KVMAGMTSLEEVNRITKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=409960 GQR90_RS03715 WP_158772935.1 856761..858497(-) (pilB) [Cobetia sp. L2A1]
ATGCCTCAGACAAATCACACGCTTAAAGGGTTTGCCGCTAGCCTGGTCGAGGCTGGCTTGTTGGAGGCTCAGGCTGCCAA
GCTTGCCGAGCAGGAAGCCAACGAGGCCGGAGAAAGCCTCTTGCTACATGTGATTACGCATGGCTTGGCACCGGCACGTG
AAGCCACCTTGGCGGCGGCATGGGAGTACGGCCTGCCCTATGTAGATATCGATGCGCTGCGTATCGACTCTCTCCCTTCT
ATAGACGAGCTTCCTGAGAAGGTGTTGCGCAAGTTAGGCGTACTGCCCCTTTATCGCCGCGAGCATCGCCTGACAGTGGG
TGTGCCCTCGCCTTCGACGCTGACACATCTTGATGAGCTGCAATTCGCCACCGGACTGTCAATCGAGAGCGTACTGGTCG
CTGCCGACCAACTGACACCTGCCCTCGAGCAATATCTGGAGGCTGGCGAAGAAAGCGCCATGGATATGCTCGGCGAGGAA
GGCGAGGCACTCGATAATCTTGAATTTGAGAACGAGGAAGATAAAAGTAATGATCATGCCATCACAGCTAATAGTGATGA
TGCCCCGGTCGTGCGTTTCGTTCACAAGGTACTCCTCGATGCCATCAAGCGTGGCGCCTCGGATATCCACTTCGAACCCT
ACGAATCCACCTATCGTATTCGTTTCCGCATCGATGGCATGCTGATCGAGAACACACGACCGCCCTTCAACATGCGTAAT
CGTATTTCGGCACGCCTGAAAGTGATGTCGCGGATGGATATCGCCGAGCGACGCCTGCCGCAGGATGGTGCCATCAAGCT
GCGAATTTCCAAGTCGCGCTCCATCGACTTCCGCGTCAATAGCTTGCCAACGGTGTATGGCGAAAAGCTGGTACTGCGTA
TTCTCGACCCTAGCTCTGCCAAGATGGGGATTGAGGCTCTGGGCTTCGAGCCTGAACAGCGCGCCATGTTCGAGAAGACA
CTGGATGAACCACAAGGGATGATTCTCGTCACTGGGCCAACGGGTAGCGGCAAGACGGTCACCCTTTATACCGGCTTGAA
TATCCTCAACACCGCTGAGCGCAATATCTCGACCGCGGAAGACCCGGTCGAGCTCAAGGTCGAAGGCGTCAATCAGGTCA
ATGTACTGCCCAAGATCGGGCTGGATTTTGCCTCGGCCTTGCGTGCCTTCCTGCGCCAGGATCCTGATGTGGTGATGGTC
GGTGAGATTCGTGACAAGGAAACCGCCGAAATAGCCATCAAAGCGGCACAGACAGGTCACCTGGTACTGTCCACGCTACA
TACCAACTCGGCGCCAGAAACACTGACACGCCTCAGTAACATGGGCATAGCGCCCTTCAATATTGCCAGTTCGGTCTCGC
TGATCATCGCGCAGCGACTGGCCCGCCGTCTGTGTACTCACTGCCGTCAACCTGCCGAGATTCCTCCTGAAGCTCTGGTA
TCGATGGGTTTCAGTACAGAGAACGGCTTTAGCGCTGAGGAGCTTGAGAGCGCAACCATTCATCGCCCAGTCGGTTGCAG
TCAATGCACACTTGGCTACAAGGGGCGTGTGGGTATCTATGAAGTCATGCCTATCTCGGATGAGATGAGTCGTTTGATCA
TGCGTGAAGGCAGTGCAATCGAATTGGCGGATCAGGCACGCAAGGAAGGGCTTTCTGATCTAAGGCGTAGCGGGCTACGC
AAGGTAATGGCTGGCATGACGTCACTTGAAGAGGTCAACCGCATTACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.033

99.827

0.529
  pilB Acinetobacter baylyi ADP1

51.652

99.481

0.514
  pilB Legionella pneumophila strain ERS1305867

51.478

99.481

0.512
  pilB Vibrio campbellii strain DS40M4

48.007

99.827

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

48.07

98.616

0.474
  pilB Vibrio cholerae strain A1552

47.314

99.827

0.472
  pilF Neisseria gonorrhoeae MS11

50.501

86.332

0.436
  pilB/pilB1 Synechocystis sp. PCC 6803

40

100

0.422
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.328

99.308

0.381
  pilF Thermus thermophilus HB27

38.23

97.751

0.374

Multiple sequence alignment