Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GQR50_RS02120 Genome accession   NZ_CP046954
Coordinates   453474..455180 (-) Length   568 a.a.
NCBI ID   WP_146061427.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain HX-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448474..460180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQR50_RS02090 (GQR50_02090) mutT 449150..449563 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  GQR50_RS02095 (GQR50_02095) yacG 449624..449818 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  GQR50_RS02100 (GQR50_02100) zapD 449828..450550 (-) 723 WP_024945431.1 cell division protein ZapD -
  GQR50_RS02105 (GQR50_02105) coaE 450588..451202 (-) 615 WP_158196555.1 dephospho-CoA kinase -
  GQR50_RS02110 (GQR50_02110) pilD 451222..452094 (-) 873 WP_158196556.1 A24 family peptidase Machinery gene
  GQR50_RS02115 (GQR50_02115) pilC 452117..453358 (-) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  GQR50_RS02120 (GQR50_02120) pilB 453474..455180 (-) 1707 WP_146061427.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GQR50_RS02125 (GQR50_02125) - 455184..455618 (-) 435 WP_158196557.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GQR50_RS02130 (GQR50_02130) nadC 455941..456798 (-) 858 WP_158196558.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GQR50_RS02135 (GQR50_02135) - 456804..457274 (-) 471 WP_171279818.1 TIGR02281 family clan AA aspartic protease -
  GQR50_RS02140 (GQR50_02140) ampD 457422..457994 (+) 573 WP_158196560.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GQR50_RS02145 (GQR50_02145) pdhR 458270..459034 (+) 765 WP_158196561.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62481.87 Da        Isoelectric Point: 5.4342

>NTDB_id=409640 GQR50_RS02120 WP_146061427.1 453474..455180(-) (pilB) [Aeromonas hydrophila strain HX-3]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=409640 GQR50_RS02120 WP_146061427.1 453474..455180(-) (pilB) [Aeromonas hydrophila strain HX-3]
ATGACCTCTAGCCCCAATAGCGGCTTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGTCAAGCCAAGGCCCAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGTTGATTGAAAAACATCATGTCTTGCCCATCTACACCCAGGGACACACCCTCTACATCGCCATGTCGGA
TCCGACCAATGTGTCGGCGCTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAAGCGCTGCTGGTAGAGGAGAACA
AACTCACCGCAGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAAGTGTCTGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCCGACGACGACGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGTATTCTGCATGAAATAGCGACGCCACCGGTCAATCTGGCCAATCGCTTCTCGGCCCGC
CTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCACAGGATGGCCGCATCAAGCTGAAACTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAAATCGTTATCCGTCTGCTCGACTCCTCAG
CGGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGCCAGAAGGCGCAATACCTGCGTGCCCTCTCGAAGCCTCAA
GGCATGATCCTGGTGACCGGCCCTACCGGCTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACAGCCGAAGATCCGGTGGAAATCAACCTACCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCCAGCGCCCTACGCTCTTTCTTGCGACAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAGACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTCCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTAGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCAGAAGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCAGTTGGCTGCAAAGAGTGCTCGGGTGGGTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAATATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375

Multiple sequence alignment