Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GQA94_RS08045 Genome accession   NZ_CP046902
Coordinates   1722120..1723823 (+) Length   567 a.a.
NCBI ID   WP_158187520.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain PM101005     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1717120..1728823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQA94_RS08030 (GQA94_08030) cysN 1717393..1719291 (+) 1899 WP_158187517.1 sulfate adenylyltransferase subunit CysN -
  GQA94_RS08035 (GQA94_08035) - 1719715..1721376 (-) 1662 WP_158187518.1 O-antigen ligase family protein -
  GQA94_RS08040 (GQA94_08040) pilA/pilAI 1721452..1721895 (-) 444 WP_158187519.1 pilin Machinery gene
  GQA94_RS08045 (GQA94_08045) pilB 1722120..1723823 (+) 1704 WP_158187520.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GQA94_RS08050 (GQA94_08050) pilC 1723825..1725042 (+) 1218 WP_158187521.1 type II secretion system F family protein Machinery gene
  GQA94_RS08055 (GQA94_08055) pilD 1725046..1725912 (+) 867 WP_158187522.1 A24 family peptidase Machinery gene
  GQA94_RS08060 (GQA94_08060) coaE 1726356..1726964 (+) 609 WP_158187523.1 dephospho-CoA kinase -
  GQA94_RS08065 (GQA94_08065) yacG 1726961..1727158 (+) 198 WP_102836851.1 DNA gyrase inhibitor YacG -
  GQA94_RS08070 (GQA94_08070) - 1727208..1727903 (-) 696 WP_158187524.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61796.62 Da        Isoelectric Point: 5.2105

>NTDB_id=409226 GQA94_RS08045 WP_158187520.1 1722120..1723823(+) (pilB) [Stutzerimonas stutzeri strain PM101005]
MADDVALSGLAKQMVLSGLLDDKTALHAQQQAARNKLSLVTYVVQNKLANGRAVSELAADQFGVAYMDLDALDKESLPKD
IVSEKLARQHRALPLIKRGNKLFVAVSDPTNHQAVTDIQFSTGLTTEAVLVEDDKLGRALDKFFESATSGLEGLGDVDLD
GVDVQTVSDDGVSDDAGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRFRTDGILHEIARPPIQLAPRISAR
LKVMAGLDISERRRPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKVVMRILDPSSAQMGIDALGYEEDQKALYMQALQQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVDLPRDALLEEGFP
ADRIGTFKIYGPVGCDNCTGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=409226 GQA94_RS08045 WP_158187520.1 1722120..1723823(+) (pilB) [Stutzerimonas stutzeri strain PM101005]
ATGGCGGACGACGTCGCCCTTTCCGGCCTTGCTAAACAAATGGTGCTGAGCGGCCTGCTCGATGACAAGACGGCGCTTCA
CGCGCAACAGCAGGCAGCGCGCAACAAGCTGTCGCTGGTGACCTATGTGGTACAAAACAAGCTCGCGAATGGACGTGCGG
TCTCGGAGCTAGCGGCCGACCAGTTTGGCGTGGCCTATATGGATCTCGACGCGCTGGACAAGGAAAGTCTGCCAAAGGAT
ATCGTTAGCGAGAAGCTGGCGCGCCAGCATCGAGCTTTGCCGCTGATCAAGCGTGGCAATAAGCTCTTTGTAGCGGTCTC
CGACCCGACCAATCACCAAGCCGTTACAGATATTCAGTTCAGTACAGGTCTGACCACCGAGGCGGTTCTGGTGGAAGACG
ACAAGCTTGGGCGGGCTCTCGACAAGTTTTTTGAAAGCGCGACGTCTGGCCTAGAGGGCCTCGGTGATGTGGATCTGGAC
GGTGTGGACGTCCAAACCGTGTCGGATGACGGCGTAAGCGATGATGCTGGTGAGGCGGCCGACGACGCGCCAGTCGTGCG
TTTCGTCAATAAGATGCTGCTCGATGCCATTCGCGGCGGGTCTTCCGATTTGCACTTCGAACCCTACGAGAAAGCCTACC
GGGTCCGGTTCCGTACGGACGGTATCCTGCATGAAATCGCCCGTCCTCCAATTCAGTTGGCGCCGCGAATCTCCGCACGG
CTGAAGGTAATGGCGGGCCTGGATATTTCCGAGCGCCGTAGGCCGCAAGATGGTCGGATCAAGATGAAGCTATCGAAGAC
CAAGGCTATCGACTTCCGTGTGAATACCCTGCCTACCTTGTGGGGCGAGAAGGTCGTGATGCGGATTCTCGACCCCAGCA
GTGCGCAGATGGGCATCGATGCGTTGGGCTATGAAGAAGATCAAAAGGCGCTGTACATGCAGGCGCTCCAGCAGCCGCAA
GGCATGATCCTGGTAACCGGGCCTACCGGATCAGGCAAAACGGTTTCCCTTTATACCGGCTTGAACATCCTCAATACGGT
CGATGTGAATATCTCCACGGCGGAAGATCCGGTGGAAATCAACCTGGAGGGGATCAACCAGGTGAACGTCAACCCCAAGC
AGGGTATGGATTTTTCCCAGGCGCTACGTGCGTTTCTGCGCCAGGACCCGGACATCATCATGGTCGGGGAAATACGTGAC
TTGGAGACGGCCGAGATTGCGATCAAGGCAGCGCAAACCGGCCACATGGTGATGTCGACGCTTCACACCAACAGTGCGGC
GGAAACCCTGACGCGCCTGCGCAACATGGGTGTGGCGGCGTTCAATATCGCGACTTCGGTGAACCTGATCATCGCCCAGC
GGCTTGCGCGCAAGCTGTGCAATAGTTGTAAAAAGGAAGTCGATCTGCCGCGTGACGCTTTGCTGGAGGAGGGCTTCCCG
GCGGACAGGATCGGAACCTTCAAAATCTACGGTCCGGTCGGCTGTGACAATTGCACCGGCGGATACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCACTGGCCAGGATTATCATGGAGGACGGCAACTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAATGACCTGCGTACGTCGGCTTTGCTGAAAGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.106

99.471

0.598
  pilB Acinetobacter baylyi ADP1

58.511

99.471

0.582
  pilB Legionella pneumophila strain ERS1305867

54.991

100

0.554
  pilF Neisseria gonorrhoeae MS11

49.556

99.295

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

50.092

96.12

0.481
  pilB Vibrio campbellii strain DS40M4

48.142

99.647

0.48
  pilB Vibrio cholerae strain A1552

52.224

91.182

0.476
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.076

99.295

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

39.499

91.534

0.362

Multiple sequence alignment