Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GQA94_RS08050 Genome accession   NZ_CP046902
Coordinates   1723825..1725042 (+) Length   405 a.a.
NCBI ID   WP_158187521.1    Uniprot ID   A0A6I6LHN7
Organism   Stutzerimonas stutzeri strain PM101005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1718825..1730042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQA94_RS08035 (GQA94_08035) - 1719715..1721376 (-) 1662 WP_158187518.1 O-antigen ligase family protein -
  GQA94_RS08040 (GQA94_08040) pilA/pilAI 1721452..1721895 (-) 444 WP_158187519.1 pilin Machinery gene
  GQA94_RS08045 (GQA94_08045) pilB 1722120..1723823 (+) 1704 WP_158187520.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GQA94_RS08050 (GQA94_08050) pilC 1723825..1725042 (+) 1218 WP_158187521.1 type II secretion system F family protein Machinery gene
  GQA94_RS08055 (GQA94_08055) pilD 1725046..1725912 (+) 867 WP_158187522.1 A24 family peptidase Machinery gene
  GQA94_RS08060 (GQA94_08060) coaE 1726356..1726964 (+) 609 WP_158187523.1 dephospho-CoA kinase -
  GQA94_RS08065 (GQA94_08065) yacG 1726961..1727158 (+) 198 WP_102836851.1 DNA gyrase inhibitor YacG -
  GQA94_RS08070 (GQA94_08070) - 1727208..1727903 (-) 696 WP_158187524.1 energy-coupling factor ABC transporter permease -
  GQA94_RS08075 (GQA94_08075) - 1728019..1729056 (-) 1038 WP_158187525.1 NADP(H)-dependent aldo-keto reductase -
  GQA94_RS08080 (GQA94_08080) rplM 1729450..1729878 (+) 429 WP_019342644.1 50S ribosomal protein L13 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43918.60 Da        Isoelectric Point: 9.8421

>NTDB_id=409227 GQA94_RS08050 WP_158187521.1 1723825..1725042(+) (pilC) [Stutzerimonas stutzeri strain PM101005]
MAQKAVKTSVFTWEGTNKQGAKIKGELSGISPALVKAQLRKQGVNPQKVRKKSVSLFSAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDDLKQDVAAGNSFAAALRKKPQYFDDLYCNLVDSGEQSGALESLLDRVATYKEKT
EALKAKIKKAMNYPIAVVLIAVIVSAILLIKVVPQFQEVFANFGAELPAFTLMVIGISEALQTWWHVVLLGLFGTAYAFK
TAHGKSEGFRNWFDRFLLKVPVVGDILYKSAVARFARTLATTFAAGVPLVDALDSVAGATGNVVFRNATMKVKNDVSSGM
QLNFSMRTTGTFPSMAIQMTAIGEESGSLDEMLAKVATFYEDEVDNMVDGLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=409227 GQA94_RS08050 WP_158187521.1 1723825..1725042(+) (pilC) [Stutzerimonas stutzeri strain PM101005]
ATGGCGCAGAAAGCGGTAAAAACCAGTGTCTTTACCTGGGAAGGGACGAACAAGCAGGGCGCAAAGATAAAAGGCGAGCT
CAGCGGTATCAGCCCCGCGCTGGTCAAGGCGCAACTTCGCAAGCAGGGCGTCAATCCGCAGAAGGTCCGTAAAAAGTCGG
TATCGCTGTTCAGTGCCGGCAAGAAGATCAAGCCGATGGACATTGCCCTGTTTACTCGCCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGTTGTTGCAGTCCTTCGACATCATCGGCGAGGGGTTCGATAACCCGAACATGCGAAAGCTGGTGGACGA
TCTGAAGCAGGATGTGGCGGCAGGCAACAGCTTTGCGGCGGCGCTGCGCAAAAAGCCTCAGTACTTCGATGATCTGTATT
GCAACCTGGTCGACTCGGGTGAGCAGTCGGGCGCGCTGGAAAGCTTGCTCGATCGCGTCGCGACGTACAAGGAAAAGACC
GAGGCGCTGAAGGCGAAGATCAAAAAAGCGATGAACTACCCCATCGCGGTGGTGCTTATTGCTGTCATCGTGTCAGCGAT
ACTGCTCATAAAGGTCGTTCCGCAGTTTCAGGAGGTCTTCGCCAATTTCGGTGCTGAGCTGCCCGCGTTTACGTTAATGG
TCATCGGAATTTCAGAAGCGCTACAGACCTGGTGGCACGTGGTATTGCTTGGCTTGTTCGGTACTGCTTATGCGTTTAAA
ACGGCTCATGGTAAGTCCGAGGGTTTTCGTAACTGGTTTGATCGGTTCTTGCTCAAGGTTCCGGTTGTAGGTGACATTCT
CTACAAATCGGCGGTGGCGCGTTTCGCTCGCACGTTGGCGACTACCTTTGCTGCCGGTGTGCCGCTTGTTGATGCGTTGG
ACTCGGTCGCAGGTGCTACCGGCAACGTGGTATTTCGCAACGCTACGATGAAGGTCAAGAACGATGTTTCCAGTGGCATG
CAGCTGAATTTCTCCATGCGAACCACTGGAACCTTCCCGAGCATGGCGATCCAGATGACAGCCATTGGCGAGGAGTCAGG
TTCTCTGGATGAGATGCTTGCGAAGGTCGCAACTTTTTATGAGGACGAAGTGGATAACATGGTAGATGGGCTCACTGCCC
TGATGGAGCCGATGATTATGGCGGTTCTTGGCGTGTTGGTCGGGGGCTTGATCATTGCGATGTACCTGCCGATTTTCCAG
CTAGGGGCTGTGGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6LHN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

90.123

100

0.901
  pilC Acinetobacter baumannii D1279779

57.531

100

0.575
  pilC Acinetobacter baylyi ADP1

57.389

100

0.575
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

44.527

99.259

0.442
  pilG Neisseria meningitidis 44/76-A

44.527

99.259

0.442
  pilC Vibrio cholerae strain A1552

41.919

97.778

0.41
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

37.093

98.519

0.365

Multiple sequence alignment