Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   METME_RS02285 Genome accession   NC_015572
Coordinates   492332..494041 (+) Length   569 a.a.
NCBI ID   WP_013817175.1    Uniprot ID   G0A291
Organism   Methylomonas methanica MC09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 487332..499041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  METME_RS02275 (Metme_0457) - 489979..491421 (+) 1443 WP_013817173.1 HDOD domain-containing protein -
  METME_RS02280 (Metme_0458) - 491425..492222 (+) 798 WP_013817174.1 response regulator -
  METME_RS02285 (Metme_0459) pilB 492332..494041 (+) 1710 WP_013817175.1 type IV-A pilus assembly ATPase PilB Machinery gene
  METME_RS02290 (Metme_0460) pilC 494045..495265 (+) 1221 WP_013817176.1 type II secretion system F family protein Machinery gene
  METME_RS02295 (Metme_0461) pilD 495347..496213 (+) 867 WP_013817177.1 A24 family peptidase Machinery gene
  METME_RS02300 (Metme_0462) coaE 496217..496828 (+) 612 WP_013817178.1 dephospho-CoA kinase -
  METME_RS02305 (Metme_0463) zapD 496825..497595 (+) 771 WP_013817179.1 cell division protein ZapD -
  METME_RS02310 (Metme_0464) - 497595..497798 (+) 204 WP_013817180.1 DNA gyrase inhibitor YacG -
  METME_RS02315 (Metme_0465) - 497808..498743 (-) 936 WP_013817181.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62545.00 Da        Isoelectric Point: 5.5853

>NTDB_id=40917 METME_RS02285 WP_013817175.1 492332..494041(+) (pilB) [Methylomonas methanica MC09]
MATAPSNIHFSGLAKCLIQQGVLTEETATAAVEEAQKNKLPFVSYLVKNKQVDSRTVANLASAEFGVPLLDLDAVDLSAV
PVKLVSEKLIRQHHALPLFKRGNRLFIATADPTNFQALDEIKFHTRLNTENILVEEEKLMRAIDAALEAVDTTMGNLLDS
DLDNIDISGGDEQPVEDTASDVDDAPIVRFVNKILLDSIKKGVSDIHLEPFEKKFRIRFRADGILSEVASPPVGISNRII
SRIKVMSKMDIAERRVPQDGRIKMQLSKNRAIDFRVNTCPTLFGEKVVMRILDPTSAQIGIEKLGFEEAQQKLFLSAIHR
PYGMVLVTGPTGSGKTVSLYTGLNILNSIERNISTAEDPVEITVEGINQVNVNPKAGLTFAEALRAFLRQDPDIIMVGEI
RDLETASIAVKAAQTGHLVLSTLHTNDAPQTINRLMQMGIEPFNIVSAVNLIMAQRLARRLCEYCKKEADFPDSVLLSAG
YSQEDIGTFKVYEPVGCEHCTNGYKGRVGIYQVLPISEKMRALILSGGNAMQMAELAKSEGINDLRASGLLKVRQGITSL
EEIDRVTKE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=40917 METME_RS02285 WP_013817175.1 492332..494041(+) (pilB) [Methylomonas methanica MC09]
ATGGCGACAGCACCTTCCAATATACATTTTAGCGGTTTGGCAAAATGCCTGATTCAGCAAGGTGTGCTTACCGAGGAGAC
TGCAACGGCTGCCGTCGAAGAAGCGCAAAAAAATAAACTGCCTTTTGTCAGCTATTTAGTCAAAAACAAGCAGGTGGATA
GCCGCACAGTGGCAAATTTGGCTTCTGCGGAATTCGGCGTACCGTTGCTGGATTTGGATGCGGTGGATTTAAGCGCAGTC
CCGGTCAAACTGGTGAGCGAAAAGTTGATTCGCCAACACCATGCCTTACCGTTATTTAAGCGCGGCAATCGTTTATTTAT
TGCGACCGCGGATCCCACCAATTTTCAGGCCCTGGATGAAATAAAATTTCATACCCGACTGAATACTGAAAATATACTGG
TAGAAGAAGAAAAACTCATGAGAGCTATCGACGCCGCTTTGGAGGCTGTCGATACAACCATGGGGAATTTACTGGACTCC
GACCTGGATAATATCGACATTTCCGGGGGGGATGAGCAGCCGGTTGAAGACACCGCATCTGATGTGGATGATGCGCCTAT
CGTGCGCTTCGTCAACAAAATTTTGTTGGATTCGATTAAAAAAGGTGTCTCGGATATTCACCTTGAGCCGTTTGAAAAAA
AGTTTCGTATTCGGTTTCGCGCGGACGGTATTTTATCGGAGGTGGCCAGTCCGCCGGTCGGTATTTCCAACCGGATTATT
TCCCGGATTAAGGTTATGTCCAAGATGGATATTGCCGAACGGCGGGTGCCGCAGGATGGTAGGATCAAGATGCAGTTATC
CAAAAATCGCGCCATCGACTTCCGGGTAAATACCTGTCCGACTTTGTTCGGCGAAAAAGTGGTGATGCGGATTCTCGACC
CGACCAGTGCTCAGATAGGCATTGAAAAACTTGGGTTTGAAGAGGCTCAACAAAAACTGTTTTTGTCCGCAATTCATCGG
CCGTATGGCATGGTGCTGGTAACAGGACCGACCGGTAGCGGTAAAACCGTATCCTTGTATACCGGATTAAATATTCTGAA
TAGTATCGAGCGCAACATTTCGACGGCCGAAGACCCCGTGGAAATCACGGTAGAAGGCATAAATCAGGTAAACGTTAATC
CGAAGGCCGGCTTGACATTTGCCGAGGCGTTACGCGCCTTCCTGCGGCAAGACCCGGACATCATCATGGTGGGGGAGATA
CGGGATTTGGAAACCGCCAGTATCGCCGTAAAAGCCGCGCAAACCGGTCACTTGGTGTTATCTACTTTGCATACCAATGA
TGCGCCGCAAACTATCAATCGCTTAATGCAAATGGGCATCGAGCCCTTCAATATCGTTTCGGCGGTTAATTTGATTATGG
CGCAACGTTTGGCGCGCCGGTTATGCGAGTACTGTAAGAAAGAGGCCGACTTTCCGGATAGTGTGTTGTTGTCCGCAGGC
TATAGTCAGGAAGATATCGGAACATTCAAAGTATATGAACCTGTGGGTTGCGAACATTGTACCAACGGCTATAAGGGCCG
AGTCGGTATTTATCAAGTGTTGCCGATCAGCGAAAAAATGCGCGCACTGATCTTAAGCGGCGGTAATGCCATGCAAATGG
CGGAACTGGCGAAATCCGAAGGCATTAATGATCTAAGAGCATCGGGGTTGCTAAAAGTGCGCCAAGGCATTACCTCGCTG
GAAGAAATAGATCGCGTCACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-146)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0A291

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56.469

100

0.568
  pilB Acinetobacter baumannii D1279779

52.715

100

0.529
  pilB Acinetobacter baylyi ADP1

52.931

98.946

0.524
  pilF Neisseria gonorrhoeae MS11

48.746

98.067

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

47.95

98.594

0.473
  pilB Vibrio campbellii strain DS40M4

46.631

99.121

0.462
  pilB Vibrio cholerae strain A1552

51.282

89.104

0.457
  pilF Thermus thermophilus HB27

39.606

98.067

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

39.273

96.661

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.698

92.97

0.369

Multiple sequence alignment