Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   METME_RS02295 Genome accession   NC_015572
Coordinates   495347..496213 (+) Length   288 a.a.
NCBI ID   WP_013817177.1    Uniprot ID   -
Organism   Methylomonas methanica MC09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 490347..501213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  METME_RS02280 (Metme_0458) - 491425..492222 (+) 798 WP_013817174.1 response regulator -
  METME_RS02285 (Metme_0459) pilB 492332..494041 (+) 1710 WP_013817175.1 type IV-A pilus assembly ATPase PilB Machinery gene
  METME_RS02290 (Metme_0460) pilC 494045..495265 (+) 1221 WP_013817176.1 type II secretion system F family protein Machinery gene
  METME_RS02295 (Metme_0461) pilD 495347..496213 (+) 867 WP_013817177.1 A24 family peptidase Machinery gene
  METME_RS02300 (Metme_0462) coaE 496217..496828 (+) 612 WP_013817178.1 dephospho-CoA kinase -
  METME_RS02305 (Metme_0463) zapD 496825..497595 (+) 771 WP_013817179.1 cell division protein ZapD -
  METME_RS02310 (Metme_0464) - 497595..497798 (+) 204 WP_013817180.1 DNA gyrase inhibitor YacG -
  METME_RS02315 (Metme_0465) - 497808..498743 (-) 936 WP_013817181.1 Nudix family hydrolase -
  METME_RS02320 (Metme_0466) argJ 498744..499958 (-) 1215 WP_013817182.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  METME_RS02325 (Metme_0467) - 499979..500203 (-) 225 WP_013817183.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31929.09 Da        Isoelectric Point: 7.3013

>NTDB_id=40919 METME_RS02295 WP_013817177.1 495347..496213(+) (pilD) [Methylomonas methanica MC09]
MFLTALQQSPGLLIALVSIVGLMIGSFLNVVIYRLPVMMQGGWRRECLEYLQMPAEEVGETFNLLWPGSRCPVCKTEIKA
YQNIPVLSYLWLRGKCSHCSAPISLRYPLIEAFTGLCSALVAWHFGYGLPMLFGLLLTWSLIALSFIDIDHQLLPDSITL
PVLWLGLILSLFSIYTDAHASIIGAVAGYLSLWSVYHLFKLLTGKEGMGFGDFKLLALLGAWLGWQYLPLIIILSSLVGA
CIGIAMIIFRQHEASRPIPFGPYLAAAGWLALIWGSDLNRLYLNYAGL

Nucleotide


Download         Length: 867 bp        

>NTDB_id=40919 METME_RS02295 WP_013817177.1 495347..496213(+) (pilD) [Methylomonas methanica MC09]
ATGTTTTTAACGGCCTTGCAACAATCCCCGGGTTTGCTTATCGCGTTGGTCTCAATAGTGGGGTTAATGATAGGCAGTTT
TTTGAATGTGGTGATTTACCGGCTACCGGTCATGATGCAAGGCGGCTGGCGCCGCGAATGTTTGGAATATTTGCAAATGC
CGGCGGAAGAAGTGGGTGAGACTTTTAATCTATTGTGGCCCGGCTCTCGTTGTCCTGTGTGTAAAACCGAGATTAAGGCC
TATCAGAATATCCCCGTGCTCAGTTACCTTTGGTTACGCGGTAAATGCAGTCATTGTTCTGCGCCTATTTCTTTGCGCTA
TCCGCTGATTGAAGCCTTTACCGGATTATGCTCCGCACTAGTTGCCTGGCATTTCGGTTACGGTTTACCCATGCTGTTCG
GTTTGCTGCTGACCTGGAGCTTGATCGCGCTGAGTTTTATCGATATCGATCATCAATTATTGCCGGATTCCATCACCTTG
CCGGTTTTGTGGCTGGGTTTGATTTTAAGTCTGTTTTCTATTTATACCGATGCGCATGCCAGCATAATTGGGGCCGTTGC
CGGTTATTTGAGTCTTTGGAGCGTTTATCACCTATTTAAGTTACTGACGGGCAAGGAAGGCATGGGCTTTGGCGACTTTA
AGCTTTTGGCTTTACTAGGCGCTTGGTTGGGATGGCAGTATTTACCTTTGATTATCATTTTGTCGTCTTTGGTGGGCGCC
TGCATCGGCATTGCCATGATTATATTCAGGCAACACGAAGCCAGTCGTCCGATTCCCTTTGGACCTTATTTGGCCGCCGC
CGGTTGGTTGGCCTTGATTTGGGGTAGTGATTTAAATCGCCTATACCTGAATTACGCCGGGTTGTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-125)

Type IV leader peptidase family (Peptidase_A24)

(136-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

54.643

97.222

0.531
  pilD Acinetobacter nosocomialis M2

54.286

97.222

0.528
  pilD Vibrio cholerae strain A1552

53.381

97.569

0.521
  pilD Vibrio campbellii strain DS40M4

52.465

98.611

0.517
  pilD Neisseria gonorrhoeae MS11

51.55

89.583

0.462

Multiple sequence alignment