Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   METME_RS02290 Genome accession   NC_015572
Coordinates   494045..495265 (+) Length   406 a.a.
NCBI ID   WP_013817176.1    Uniprot ID   G0A292
Organism   Methylomonas methanica MC09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 489045..500265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  METME_RS02275 (Metme_0457) - 489979..491421 (+) 1443 WP_013817173.1 HDOD domain-containing protein -
  METME_RS02280 (Metme_0458) - 491425..492222 (+) 798 WP_013817174.1 response regulator -
  METME_RS02285 (Metme_0459) pilB 492332..494041 (+) 1710 WP_013817175.1 type IV-A pilus assembly ATPase PilB Machinery gene
  METME_RS02290 (Metme_0460) pilC 494045..495265 (+) 1221 WP_013817176.1 type II secretion system F family protein Machinery gene
  METME_RS02295 (Metme_0461) pilD 495347..496213 (+) 867 WP_013817177.1 A24 family peptidase Machinery gene
  METME_RS02300 (Metme_0462) coaE 496217..496828 (+) 612 WP_013817178.1 dephospho-CoA kinase -
  METME_RS02305 (Metme_0463) zapD 496825..497595 (+) 771 WP_013817179.1 cell division protein ZapD -
  METME_RS02310 (Metme_0464) - 497595..497798 (+) 204 WP_013817180.1 DNA gyrase inhibitor YacG -
  METME_RS02315 (Metme_0465) - 497808..498743 (-) 936 WP_013817181.1 Nudix family hydrolase -
  METME_RS02320 (Metme_0466) argJ 498744..499958 (-) 1215 WP_013817182.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  METME_RS02325 (Metme_0467) - 499979..500203 (-) 225 WP_013817183.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44247.07 Da        Isoelectric Point: 7.1412

>NTDB_id=40918 METME_RS02290 WP_013817176.1 494045..495265(+) (pilC) [Methylomonas methanica MC09]
MAKQTDQIDFLWEGLDKQSKKTNGLISAKSESIARTELRKMGVRVVKIKPKPKPLFGAKTQKITTGDIAVFARQLATMLE
AGVPLVQSFDIIGKGHDNASMSELLLGIKADIEGGDTLAQALGKRPIYFDDLFCNLVNAGEQAGVLEGLLDKIATYKEKT
ESIRKKVKKALTYPIAVLVVAFIVTAILLIFVVPVFEDLFKGFGADLPAFTKFVISLSEWVQEWWWAVVGVLGGLGYTFG
YFKKRSRPFNHFLDRTLLKIPVVGMILEKSAIARFARTLSTMSAAGVPLVEALVSVAGACGNILFYDGVMKMRDDVSTGQ
QLQFSMVQTGLFPNMVVQMVAIGEESGSIDSMLDKVADFYEEEVDNLVDNLSSLMEPIIMAVLGVLVGGLIVAMYLPIFK
LGAAVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=40918 METME_RS02290 WP_013817176.1 494045..495265(+) (pilC) [Methylomonas methanica MC09]
ATGGCCAAGCAAACAGACCAAATCGACTTTCTCTGGGAAGGTCTCGATAAACAGAGTAAAAAAACCAATGGCTTGATCAG
TGCCAAGAGTGAAAGTATCGCCCGCACCGAACTCAGGAAAATGGGGGTGAGGGTCGTAAAAATAAAACCCAAGCCCAAAC
CGCTGTTTGGTGCAAAAACCCAAAAAATCACTACAGGGGATATTGCAGTATTTGCCCGGCAATTGGCAACCATGCTTGAG
GCGGGCGTGCCCTTGGTACAATCGTTCGACATTATCGGCAAAGGCCACGACAATGCCAGTATGTCGGAGTTGTTGTTGGG
TATTAAAGCCGATATCGAAGGCGGTGATACCTTGGCTCAGGCTTTAGGCAAGCGGCCCATCTATTTTGATGATTTATTCT
GCAATCTGGTCAACGCCGGCGAGCAAGCGGGGGTGTTGGAAGGTTTGCTGGACAAAATTGCCACCTATAAGGAAAAAACC
GAGTCGATTCGTAAAAAAGTCAAAAAAGCCCTGACTTACCCGATTGCCGTGTTAGTGGTGGCTTTTATCGTCACGGCGAT
ATTGCTGATTTTTGTGGTGCCGGTGTTTGAGGATTTGTTTAAAGGTTTTGGTGCCGATTTGCCTGCGTTCACCAAATTCG
TTATCAGTTTGTCGGAATGGGTGCAGGAATGGTGGTGGGCCGTGGTAGGCGTGCTTGGCGGGCTTGGATACACCTTTGGA
TATTTCAAAAAGCGCTCGCGGCCGTTTAATCACTTTTTGGATAGGACGCTGTTGAAAATTCCGGTAGTCGGCATGATTCT
GGAAAAGTCGGCCATTGCCCGTTTCGCCCGTACGTTGTCTACCATGTCTGCCGCCGGAGTGCCATTGGTGGAGGCGCTGG
TTTCCGTCGCCGGGGCCTGCGGGAATATCCTGTTTTACGACGGCGTGATGAAAATGCGCGACGATGTGTCCACGGGTCAA
CAGTTGCAATTTTCCATGGTTCAGACCGGTTTGTTTCCGAATATGGTGGTACAGATGGTGGCGATCGGCGAAGAGTCCGG
TTCAATTGACAGCATGCTGGACAAAGTGGCCGATTTTTACGAGGAAGAGGTCGATAACTTGGTCGATAACCTGAGCAGTT
TAATGGAACCTATCATTATGGCTGTTTTGGGGGTTTTGGTGGGCGGTTTGATCGTGGCTATGTATTTGCCTATCTTCAAG
CTTGGTGCTGCGGTTGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0A292

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

61.307

98.03

0.601
  pilC Pseudomonas stutzeri DSM 10701

58.838

97.537

0.574
  pilC Acinetobacter baumannii D1279779

54.66

97.783

0.534
  pilC Acinetobacter baylyi ADP1

52.393

97.783

0.512
  pilG Neisseria meningitidis 44/76-A

47.382

98.768

0.468
  pilG Neisseria gonorrhoeae MS11

47.132

98.768

0.466
  pilC Vibrio cholerae strain A1552

41.25

98.522

0.406
  pilC Vibrio campbellii strain DS40M4

39.454

99.261

0.392

Multiple sequence alignment