Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY43_RS09475 Genome accession   NZ_CP046797
Coordinates   2061013..2062701 (+) Length   562 a.a.
NCBI ID   WP_158107764.1    Uniprot ID   -
Organism   Vibrio furnissii strain 2013V-1001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2056013..2067701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY43_RS09445 dsbC 2056334..2057098 (-) 765 WP_158107763.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  GPY43_RS09450 xerD 2057115..2058023 (-) 909 WP_004729073.1 site-specific tyrosine recombinase XerD -
  GPY43_RS09455 fldB 2058164..2058685 (+) 522 WP_004729075.1 flavodoxin FldB -
  GPY43_RS09460 ampD 2058720..2059274 (-) 555 WP_004729077.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY43_RS09465 nadC 2059423..2060310 (+) 888 WP_154180385.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY43_RS09470 - 2060557..2061006 (+) 450 WP_055466517.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY43_RS09475 pilB 2061013..2062701 (+) 1689 WP_158107764.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY43_RS09480 pilC 2062780..2064006 (+) 1227 WP_004729085.1 type II secretion system F family protein Machinery gene
  GPY43_RS09485 pilD 2064058..2064927 (+) 870 WP_004729087.1 A24 family peptidase Machinery gene
  GPY43_RS09490 coaE 2064930..2065538 (+) 609 WP_158107765.1 dephospho-CoA kinase -
  GPY43_RS09495 zapD 2065571..2066311 (+) 741 WP_004729090.1 cell division protein ZapD -
  GPY43_RS09500 yacG 2066369..2066566 (+) 198 WP_004729092.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62073.16 Da        Isoelectric Point: 6.0057

>NTDB_id=407949 GPY43_RS09475 WP_158107764.1 2061013..2062701(+) (pilB) [Vibrio furnissii strain 2013V-1001]
MLTNLIAILRQAELISPTQEQAVIDHIHASGATLPEALIDLAIFHHHDLTEQLSQIFGLPETDLTRFDYAPLCQQLGLRE
LITRYQALPLHSNGQILTLAVADPTHLQVEDEFRFATGLQVELVLADHKALHAAIRRLYGRSLATPGTQGKEISQDELAD
LVQISDDEKTTIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESHYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLDADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYNEQQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTEQVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVAITPPAALQQQFGLLAED
AVYEAHPSGCTECTNGYSGRVGIYEVMAFHPALADAVMQRASIHQIETIAQQHGMQPLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=407949 GPY43_RS09475 WP_158107764.1 2061013..2062701(+) (pilB) [Vibrio furnissii strain 2013V-1001]
ATGCTGACCAACCTTATCGCCATTCTGCGTCAGGCTGAGTTGATCAGCCCAACGCAGGAACAGGCCGTGATCGACCATAT
TCACGCTTCCGGTGCCACGCTGCCGGAGGCGTTGATCGATTTGGCGATATTTCATCATCACGATTTAACCGAGCAACTGA
GTCAGATCTTTGGTCTACCCGAAACCGATCTCACTCGGTTTGATTACGCGCCATTATGCCAGCAATTGGGACTTCGAGAA
CTCATCACCCGCTATCAAGCCCTGCCGCTACACAGCAATGGTCAAATCCTGACGTTGGCGGTTGCTGACCCAACTCACCT
GCAAGTCGAAGACGAGTTTCGCTTTGCCACCGGATTACAGGTGGAACTGGTGCTCGCCGATCACAAGGCGCTGCATGCCG
CCATTCGCCGCTTGTATGGGCGCAGCCTCGCGACGCCGGGGACGCAAGGCAAAGAGATCAGTCAGGATGAATTGGCGGAT
CTGGTACAGATCTCTGACGACGAAAAAACCACCATCGAAGATCTCAGCCAAGATGATTCACCGGTCAGTCGTTTTATCAA
CCAGATCCTACTCGATGCCGTGCGCAAAGGTGCGTCCGACATTCACTTTGAGCCGTATGAGTCCCACTACCGTATTCGTC
TGCGCTGTGACGGCATTCTGGTCGAAACCCAACAACCTGCCAGTCACCTCAGCCGACGCTTGGCAGCCCGAATCAAAATT
TTGTCGAAACTGGATATTGCCGAGCGCCGCCTCCCGCAAGATGGACGCATCAAACTGCGCTTGGATGCCGACACCGCGAT
TGATATGCGTGTATCGACCTTGCCAACATTGTGGGGTGAAAAGATTGTTCTGCGCTTACTCGATAGCAGCGCGGCCAACC
TCAACATCGACAAACTCGGCTACAACGAGCAGCAAAAGCAACTCTACCTCGCGGCCCTCACCAAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGCAGCGGCAAAACTGTGTCGCTCTACACCGGATTACGCATTCTCAATACCGAACAAGTGAA
CATCTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGTATCAATCAGGTGCAAGTTCAACCCAAAATCGGCTTTG
GCTTTGCACAAGCGCTGCGCTCGTTTCTACGCCAAGATCCCGATGTGGTGATGGTGGGCGAAATTCGCGATCTAGAAACC
GCAGAAATCGCCATTAAAGCCGCGCAAACCGGGCACTTGGTGCTCTCAACCTTGCACACCAACTCGGCCGCGGAAACCGT
GATTCGTTTAGCCAACATGGGCATTGAACCCTTTAACCTTGCCTCATCATTGAGCCTGATCATTGCGCAGCGGCTGGCCC
GCCGTCTGTGCCCGCATTGCAAGGTCGCCATCACGCCCCCAGCTGCGCTGCAGCAGCAATTTGGCTTGCTCGCCGAAGAT
GCCGTGTACGAAGCACATCCATCGGGTTGCACGGAATGCACCAATGGGTATAGCGGCCGCGTGGGCATTTATGAAGTGAT
GGCGTTTCATCCCGCCTTGGCGGACGCGGTGATGCAGCGCGCCAGCATTCATCAAATCGAAACCATTGCCCAACAGCACG
GCATGCAACCCCTGCTGCAATCGGGGATTGAAAAACTGCGCGCGGGCATCACCAGTTTTAGTGAATTGCAGCGAGTACTC
TACTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.384

100

0.824
  pilB Vibrio campbellii strain DS40M4

71.886

100

0.719
  pilB Vibrio parahaemolyticus RIMD 2210633

71.886

100

0.719
  pilB Legionella pneumophila strain ERS1305867

52.519

91.815

0.482
  pilB Acinetobacter baumannii D1279779

51.06

92.349

0.472
  pilB Acinetobacter baylyi ADP1

50.097

91.993

0.461
  pilF Neisseria gonorrhoeae MS11

45.035

100

0.452
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilF Thermus thermophilus HB27

36.833

100

0.368
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368

Multiple sequence alignment