Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GOY17_RS03960 Genome accession   NZ_CP046603
Coordinates   871745..873463 (-) Length   572 a.a.
NCBI ID   WP_157029414.1    Uniprot ID   -
Organism   Lysobacter soli strain XL170     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 866745..878463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOY17_RS03940 (GOY17_03940) - 867887..868891 (+) 1005 WP_157029410.1 Nudix family hydrolase -
  GOY17_RS03945 (GOY17_03945) coaE 868910..869527 (-) 618 WP_157029411.1 dephospho-CoA kinase -
  GOY17_RS03950 (GOY17_03950) - 869574..870437 (-) 864 WP_157029412.1 A24 family peptidase -
  GOY17_RS03955 (GOY17_03955) pilC 870483..871739 (-) 1257 WP_157029413.1 type II secretion system F family protein Machinery gene
  GOY17_RS03960 (GOY17_03960) pilB 871745..873463 (-) 1719 WP_157029414.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GOY17_RS03965 (GOY17_03965) comP 873615..874031 (-) 417 WP_157029415.1 pilin Machinery gene
  GOY17_RS03970 (GOY17_03970) pilR 874510..875970 (-) 1461 WP_157029416.1 sigma-54 dependent transcriptional regulator Regulator
  GOY17_RS03975 (GOY17_03975) - 876054..877679 (-) 1626 WP_232338523.1 ATP-binding protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62367.47 Da        Isoelectric Point: 6.0012

>NTDB_id=405988 GOY17_RS03960 WP_157029414.1 871745..873463(-) (pilB) [Lysobacter soli strain XL170]
MNAVTANLVGITGIARRLVLDGALDDTAARQAMAAAAGLKQPLTAYLAEKRLVTAPQLAAANCVEFGVPLFQVDAFDPSQ
SAIKLINEALIRKHQVLPLYRRGNRLFVGISDPTNTQALDEIKFQSNLAIEPILVSEDQIQRCIETWLEASDTLSDAVGN
DEGLENLDISGGEDVASESGVEVKTDDTPVVKFVNKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQVSKVPPNLSG
RIAARLKVMSQLDIAEKRVPQDGRIKLNISKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEDDQRALYEQA
LAKPYGMVLVTGPTGSGKTVSLYTGLNILNEEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDVVMV
GEIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTVSRLMNMGIAPYNITSSVTLIIAQRLARRLHDCKREVQLPEHALLA
EGFSHEEVAAGLKIYEAVGCSDCTEGYKGRTGIYQVMPMTEKIQSIILQGGNALDIADAAQQSGVRDLRQSALRKVKNGV
TSLAEINRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=405988 GOY17_RS03960 WP_157029414.1 871745..873463(-) (pilB) [Lysobacter soli strain XL170]
ATGAATGCCGTCACCGCCAATCTGGTGGGGATCACGGGTATCGCGCGGCGCCTGGTGCTCGATGGGGCACTGGATGACAC
GGCCGCGCGCCAAGCGATGGCCGCAGCAGCCGGGCTGAAGCAGCCGTTGACGGCTTACCTGGCCGAGAAGCGGCTCGTCA
CTGCTCCGCAGCTGGCAGCAGCCAACTGTGTCGAATTCGGCGTACCGCTTTTCCAGGTTGACGCGTTCGACCCATCGCAA
TCGGCCATCAAGCTCATCAACGAGGCATTGATCCGCAAGCACCAGGTACTCCCGCTGTACCGGCGGGGGAACCGGTTGTT
CGTAGGCATTTCCGATCCCACCAACACGCAGGCACTGGACGAGATCAAGTTCCAGTCGAACCTGGCCATCGAACCCATCC
TCGTCAGCGAAGATCAGATCCAGCGCTGCATAGAAACCTGGCTTGAAGCGAGCGACACGCTTTCTGATGCAGTCGGAAAC
GACGAAGGACTCGAAAACCTCGACATTTCCGGCGGCGAAGATGTTGCGAGCGAATCCGGCGTAGAGGTAAAGACCGACGA
CACGCCGGTTGTAAAATTCGTCAATAAAGTGCTCGTGGATGCCATTCGCCGTGGCGCGTCCGACATCCATTTCGAACCCT
ACGAAACCGAGTATCGTGTCCGTCTGCGCATCGACGGCCTGTTGAAGCAGGTCAGCAAGGTTCCGCCAAACCTCTCGGGT
CGCATTGCAGCGCGCCTCAAAGTCATGTCGCAACTCGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCGCATCAAGCT
CAACATCTCGAAGACCAAGCAGGTCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGGTGCTGCGTA
TTCTCGACGGCAGTGCGGCCAAGCTCGGCATCGACAAGCTCGGCTACGAGGACGACCAGCGCGCGCTCTACGAACAGGCG
CTGGCCAAGCCCTACGGCATGGTGCTGGTCACCGGTCCGACCGGCTCGGGCAAGACCGTCTCGCTGTACACGGGCCTGAA
CATCCTCAACGAGGAACAGCGCAACATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCAACCAGGTCC
AGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGACAGGACCCGGACGTGGTGATGGTC
GGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGGTTAAGGCGGCACAGACGGGCCATCTGGTGCTTTCGACGCTGCA
CACCAACGACGCCCCGCAGACTGTGTCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCTCATCGGTGACAC
TGATCATCGCGCAGCGTCTGGCCCGTCGACTGCACGACTGCAAGCGCGAAGTGCAGTTGCCGGAACACGCGTTGCTGGCC
GAAGGCTTCAGCCACGAGGAAGTCGCCGCAGGCCTGAAGATCTACGAAGCCGTGGGCTGCTCCGACTGTACCGAGGGGTA
CAAGGGACGCACTGGCATCTACCAGGTCATGCCGATGACGGAAAAGATCCAGTCGATCATCCTGCAGGGTGGTAATGCTC
TGGACATTGCCGATGCGGCACAGCAGTCCGGTGTACGCGACTTGCGCCAGTCTGCACTTCGTAAAGTAAAGAACGGCGTC
ACCAGCCTCGCCGAAATCAACCGTGTAACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-147)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.93

99.301

0.545
  pilB Acinetobacter baumannii D1279779

54.513

98.776

0.538
  pilB Legionella pneumophila strain ERS1305867

52.557

99.126

0.521
  pilB Vibrio cholerae strain A1552

51.657

89.685

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

47.963

94.406

0.453
  pilF Neisseria gonorrhoeae MS11

49.143

91.783

0.451
  pilB Vibrio campbellii strain DS40M4

50.392

89.161

0.449
  pilB/pilB1 Synechocystis sp. PCC 6803

37.882

97.378

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.421

91.259

0.369

Multiple sequence alignment