Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B565_RS01835 Genome accession   NC_015424
Coordinates   383894..385600 (-) Length   568 a.a.
NCBI ID   WP_013723413.1    Uniprot ID   -
Organism   Aeromonas veronii B565     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 378894..390600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B565_RS01805 (B565_0353) mutT 379404..379808 (+) 405 WP_005355106.1 8-oxo-dGTP diphosphatase MutT -
  B565_RS01810 (B565_0354) yacG 379886..380080 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  B565_RS01815 (B565_0355) zapD 380090..380812 (-) 723 WP_005355104.1 cell division protein ZapD -
  B565_RS01820 (B565_0356) coaE 380850..381464 (-) 615 WP_005355102.1 dephospho-CoA kinase -
  B565_RS01825 (B565_0357) pilD 381478..382350 (-) 873 WP_013723412.1 prepilin peptidase Machinery gene
  B565_RS01830 (B565_0358) pilC 382412..383653 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  B565_RS01835 (B565_0359) pilB 383894..385600 (-) 1707 WP_013723413.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  B565_RS20610 - 385604..386029 (-) 426 WP_041915402.1 pilin -
  B565_RS01845 (B565_0360) nadC 386345..387220 (-) 876 WP_013723414.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B565_RS01850 (B565_0361) - 387224..387694 (-) 471 WP_005355088.1 retropepsin-like aspartic protease family protein -
  B565_RS01855 (B565_0362) ampD 387825..388400 (+) 576 WP_005355087.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  B565_RS01860 (B565_0363) pdhR 388679..389443 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62519.85 Da        Isoelectric Point: 5.3170

>NTDB_id=40446 B565_RS01835 WP_013723413.1 383894..385600(-) (pilB) [Aeromonas veronii B565]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=40446 B565_RS01835 WP_013723413.1 383894..385600(-) (pilB) [Aeromonas veronii B565]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGACATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTTGATTTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATTGCCATGTCGGA
TCCGACCAATGTCTCGGCATTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAAGCGCTGCTGGTAGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGTGAAAACGACGCCCTCGGCATGGAGGATATCGACGAATCCGAAATT
TCGGATCTCGAAGTCTCCGACGAAAACAGCCGGCTGGACGAAAGCGTCAATACCGCCGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGAGCCTCAGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTTCGCTTTCGCATCGATGGCATTCTGCATGAAATTGCCACTCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTGATGGCGCGACTCGATATTGCCGAGCGGCGGTTGCCGCAGGATGGCCGCATCAAGTTGAAGTTGTCGCGCAA
TAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCCATCAAGGCGGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
TGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCGGAAGTGGTACCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCAGCCGGGCTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGACGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAATATCGCCAAATTGATCATGCAGGGCGCCAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAAGAGGGGATGCGTACCCTGCGTACCTCGGGGCTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAATCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377

Multiple sequence alignment