Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GMO17_RS01630 Genome accession   NZ_CP046441
Coordinates   352526..354631 (+) Length   701 a.a.
NCBI ID   WP_005888212.1    Uniprot ID   -
Organism   Pseudomonas coronafaciens pv. coronafaciens strain B19001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347526..359631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GMO17_RS01610 (GMO17_01685) pilM 349695..350759 (+) 1065 WP_191892739.1 pilus assembly protein PilM Machinery gene
  GMO17_RS01615 (GMO17_01690) - 350759..351334 (+) 576 WP_005888206.1 PilN domain-containing protein -
  GMO17_RS01620 (GMO17_01695) pilO 351331..351954 (+) 624 WP_005888208.1 type 4a pilus biogenesis protein PilO -
  GMO17_RS01625 (GMO17_01700) pilP 351951..352478 (+) 528 WP_005888210.1 type 4a pilus biogenesis lipoprotein PilP -
  GMO17_RS01630 (GMO17_01705) pilQ 352526..354631 (+) 2106 WP_005888212.1 type IV pilus secretin PilQ Machinery gene
  GMO17_RS01635 (GMO17_01710) aroK 354636..355154 (+) 519 WP_005888214.1 shikimate kinase AroK -
  GMO17_RS01640 (GMO17_01715) aroB 355270..356373 (+) 1104 WP_005888216.1 3-dehydroquinate synthase -
  GMO17_RS01645 (GMO17_01720) - 356384..358003 (+) 1620 WP_054997514.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75454.89 Da        Isoelectric Point: 5.1826

>NTDB_id=404280 GMO17_RS01630 WP_005888212.1 352526..354631(+) (pilQ) [Pseudomonas coronafaciens pv. coronafaciens strain B19001]
MTRIFSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLSFDAPVPAPRGYTTAQPARIALDLPGVTSQLSTKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGASAAQASQPTTAMGAQRVAVPPPTPASARPYVPA
GAKAIRNIDFQRGELGEGNVVIDLSNASIAPDIQEQGGKIRVDFAKTLLPEPLRVRLDVKDFATPVQFVSASTSGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAEAKADERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGRTNLSGSS
KWSAGGLDNNGDEAGNTGNNLGPNIPFVDLGAANATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=404280 GMO17_RS01630 WP_005888212.1 352526..354631(+) (pilQ) [Pseudomonas coronafaciens pv. coronafaciens strain B19001]
ATGACCCGGATTTTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTTTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTGGCTGCCCTGCCGGGCGATCGCATCGAGCTCAAGCTGTCATTCGATGCGCCCGTGCCTGCGCCTCGTG
GCTACACTACCGCGCAACCGGCTCGCATCGCGCTGGACCTGCCGGGCGTGACCAGCCAGTTATCGACCAAAAGTCGAGAA
CTGGGCACCGGCAATGCACGCAGTGTGGTCGTGGTGCAGGCCCAGGATCGTACGCGGGTGATCATCAACCTGACGACGCT
GTCGCCTTACAGCTCGCGGGTCGACGGCAACAACCTGTTCGTGGTCATCGGGCAAGGAGCATCTGCCGCGCAGGCCTCTC
AGCCAACCACCGCAATGGGCGCGCAGAGAGTGGCTGTGCCGCCGCCCACTCCGGCTTCCGCCAGGCCTTACGTGCCTGCC
GGCGCCAAGGCGATCAGGAATATCGACTTCCAGCGCGGCGAGTTGGGTGAAGGTAATGTGGTCATCGACCTGAGCAATGC
CAGCATTGCCCCTGATATTCAGGAGCAGGGCGGCAAGATCCGCGTCGATTTCGCCAAGACCCTGCTGCCTGAACCGCTGC
GTGTCCGCCTCGACGTCAAGGACTTCGCGACCCCCGTGCAGTTCGTCAGTGCTTCTACTTCTGGCGACAAGGCCAGCATC
TTGATCGAGCCGTCGGGCGCTTTCGATTACTCGGCGTTCCAGACCGAAAACAAGCTGACGATCAGTGTGCGTCCGTTGAC
CAACGAGGACGTGGACCGCCGCGCCGCCGACAAGCCGGTGTACACCGGTGAGAAGCTGTCGCTGAATTTCCAGGACATCG
ATGTGCGTTCGGTGCTGCAACTGATTGCCGACTTCACCAACCTTAATCTGGTCGCCAGCGACACTGTCCAGGGTGGCATC
ACGCTGCGCCTGCAGAACGTGCCGTGGGATCAGGCATTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGAT
CGGCAACGTCCTGCTGGTCGCGCCAGCTGACGAAATCGCCGCCCGCGAACGTCAGGAGCTGGAATCGCTCAAGCAGATCG
CCGAACTGGCGCCGCTGCGTCGTGAACTGCTGCAGGTCAACTACGCCAAGGCCGCCGATATCGCCAAGCTGTTCCAGTCG
GTGACCAGCGCCGAGGCCAAGGCTGACGAGCGTGGCTCCATTACAGTGGACGACCGCACCAATAACATCATTGCCTATCA
GACCCAGGACCGCCTGGACGAACTTCGCCGTATCGTTTCGCAACTGGACATTCCGGTGCGTCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAACAGTTGGGTGTGCGTTGGGGCGGCCGGACCAACCTGTCGGGTTCCAGC
AAATGGAGCGCTGGCGGTCTGGACAATAACGGCGATGAGGCAGGCAATACCGGCAACAATCTGGGGCCCAACATTCCGTT
TGTCGACCTGGGCGCTGCTAATGCGACGACAGGTATCGGCCTGGGCTTCGTGACCAACAACACGCTGCTGGACCTTGAGC
TGAGCGCGATGGAAAAAACCGGCAACGGTGAAATCGTCTCCCAGCCCAAAGTGGTCACGTCCGACAAGGAAACTGCCAAG
ATCCTCAAGGGCACGGAAATCCCGTACCAGGAATCCAGCTCCAGCGGCGCAACCACCGTGAGCTTCAAGGAGGCGTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCCGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTTCCGCCTATCAAGAAAAACGAAGTCAACGCCAAAGTGCTGATTTCCGACGGCGAAACC
ATCGTCATTGGCGGCGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTCGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.299

100

0.745
  comQ Acinetobacter baylyi ADP1

41.088

100

0.431
  pilQ Acinetobacter baumannii strain A118

38.926

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.926

100

0.414
  pilQ Legionella pneumophila strain ERS1305867

38.316

98.288

0.377
  pilQ Legionella pneumophila strain Lp02

38.171

98.288

0.375

Multiple sequence alignment