Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GNT55_RS29070 Genome accession   NZ_CP046404
Coordinates   6193063..6195207 (-) Length   714 a.a.
NCBI ID   WP_003161249.1    Uniprot ID   A0A8G2V818
Organism   Pseudomonas aeruginosa strain SE5416     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 6193063..6240425 6193063..6195207 within 0


Gene organization within MGE regions


Location: 6193063..6240425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNT55_RS29070 (GNT55_29070) pilQ 6193063..6195207 (-) 2145 WP_003161249.1 type 4a pilus secretin PilQ Machinery gene
  GNT55_RS29075 (GNT55_29075) - 6195261..6195368 (-) 108 Protein_5775 pilus assembly protein PilP -
  GNT55_RS29080 (GNT55_29080) - 6195492..6195851 (-) 360 WP_003094179.1 Mor transcription activator family protein -
  GNT55_RS29085 (GNT55_29085) - 6195854..6196417 (-) 564 WP_023657063.1 gp16 family protein -
  GNT55_RS29090 (GNT55_29090) - 6196404..6196871 (-) 468 WP_179063022.1 hypothetical protein -
  GNT55_RS29095 (GNT55_29095) - 6196873..6197091 (-) 219 WP_003148480.1 hypothetical protein -
  GNT55_RS29100 (GNT55_29100) - 6197093..6197782 (-) 690 WP_003094188.1 DUF2786 domain-containing protein -
  GNT55_RS29105 (GNT55_29105) - 6197784..6198407 (-) 624 WP_003094190.1 DUF3164 family protein -
  GNT55_RS29110 (GNT55_29110) - 6198400..6198600 (-) 201 WP_003148482.1 hypothetical protein -
  GNT55_RS32670 - 6198593..6199204 (-) 612 WP_003148484.1 hypothetical protein -
  GNT55_RS29120 (GNT55_29120) - 6199204..6199509 (-) 306 WP_003148486.1 hypothetical protein -
  GNT55_RS29125 (GNT55_29125) - 6199506..6199847 (-) 342 WP_094226439.1 hypothetical protein -
  GNT55_RS29130 (GNT55_29130) - 6199849..6201018 (-) 1170 WP_023102419.1 ExeA family protein -
  GNT55_RS29135 (GNT55_29135) - 6201018..6202796 (-) 1779 WP_094226440.1 DDE-type integrase/transposase/recombinase -
  GNT55_RS29140 (GNT55_29140) - 6202801..6203757 (-) 957 WP_023657068.1 hypothetical protein -
  GNT55_RS29145 (GNT55_29145) - 6203815..6204114 (-) 300 WP_031642102.1 helix-turn-helix domain-containing protein -
  GNT55_RS29150 (GNT55_29150) - 6204111..6204371 (-) 261 WP_058177651.1 hypothetical protein -
  GNT55_RS29155 (GNT55_29155) - 6204364..6204852 (-) 489 WP_094226441.1 hypothetical protein -
  GNT55_RS29160 (GNT55_29160) - 6204972..6205682 (-) 711 WP_179063320.1 hypothetical protein -
  GNT55_RS29165 (GNT55_29165) - 6205709..6206029 (+) 321 WP_033866801.1 hypothetical protein -
  GNT55_RS29170 (GNT55_29170) - 6206075..6206284 (-) 210 WP_023657070.1 DNA-binding protein -
  GNT55_RS32890 - 6206383..6206820 (+) 438 WP_179063041.1 helix-turn-helix domain-containing protein -
  GNT55_RS29180 (GNT55_29180) - 6206840..6207043 (+) 204 WP_094226442.1 hypothetical protein -
  GNT55_RS29185 - 6207099..6207998 (-) 900 WP_179063321.1 hypothetical protein -
  GNT55_RS29190 (GNT55_29190) - 6208172..6208468 (+) 297 WP_003152187.1 putative holin -
  GNT55_RS29195 - 6208471..6208629 (+) 159 WP_162835812.1 hypothetical protein -
  GNT55_RS29200 (GNT55_29195) - 6208626..6209255 (+) 630 WP_033866804.1 transglycosylase SLT domain-containing protein -
  GNT55_RS29205 (GNT55_29200) - 6209436..6210047 (+) 612 WP_023657073.1 hypothetical protein -
  GNT55_RS29210 (GNT55_29205) - 6210051..6210440 (+) 390 WP_003094234.1 hypothetical protein -
  GNT55_RS29215 (GNT55_29210) - 6210430..6210738 (+) 309 WP_003094236.1 hypothetical protein -
  GNT55_RS29220 (GNT55_29215) - 6210744..6211319 (+) 576 WP_003094238.1 DUF3486 family protein -
  GNT55_RS29225 (GNT55_29220) - 6211321..6213024 (+) 1704 WP_023657074.1 phage protein gp28 -
  GNT55_RS29230 (GNT55_29225) - 6213024..6214502 (+) 1479 WP_003152194.1 DUF935 domain-containing protein -
  GNT55_RS29235 (GNT55_29230) - 6214622..6215752 (+) 1131 WP_223656224.1 phage minor head protein -
  GNT55_RS29240 (GNT55_29235) - 6215749..6216321 (+) 573 WP_031630686.1 phage virion morphogenesis protein -
  GNT55_RS29245 (GNT55_29240) - 6216540..6217790 (+) 1251 WP_223697285.1 peptidase -
  GNT55_RS29250 (GNT55_29245) - 6217794..6218171 (+) 378 WP_023102437.1 capsid cement protein -
  GNT55_RS29255 (GNT55_29250) - 6218183..6219112 (+) 930 WP_179063322.1 phage capsid protein -
  GNT55_RS29260 (GNT55_29255) - 6219159..6219353 (+) 195 WP_003094256.1 hypothetical protein -
  GNT55_RS29265 (GNT55_29260) - 6219353..6219679 (+) 327 WP_003094258.1 hypothetical protein -
  GNT55_RS29270 (GNT55_29265) - 6219681..6220196 (+) 516 WP_223198311.1 DUF1320 domain-containing protein -
  GNT55_RS29275 (GNT55_29270) - 6220198..6220653 (+) 456 WP_003094263.1 hypothetical protein -
  GNT55_RS29280 (GNT55_29275) - 6220650..6220859 (+) 210 WP_003139972.1 hypothetical protein -
  GNT55_RS29285 (GNT55_29280) - 6220863..6221615 (+) 753 WP_003094267.1 hypothetical protein -
  GNT55_RS29290 (GNT55_29285) - 6221617..6222123 (+) 507 WP_179063323.1 DUF6631 family protein -
  GNT55_RS29295 - 6222120..6222263 (+) 144 WP_003094272.1 hypothetical protein -
  GNT55_RS29300 (GNT55_29290) - 6222350..6226192 (+) 3843 WP_023657077.1 phage tail length tape measure family protein -
  GNT55_RS29305 (GNT55_29295) - 6226200..6227156 (+) 957 WP_058199458.1 hypothetical protein -
  GNT55_RS29310 (GNT55_29300) - 6227158..6228081 (+) 924 WP_012613801.1 hypothetical protein -
  GNT55_RS29315 (GNT55_29305) - 6228081..6229784 (+) 1704 WP_003117242.1 hypothetical protein -
  GNT55_RS29320 (GNT55_29310) - 6229774..6230595 (+) 822 WP_179063324.1 phage BR0599 family protein -
  GNT55_RS29325 (GNT55_29315) - 6230604..6230843 (+) 240 WP_003094581.1 hypothetical protein -
  GNT55_RS29330 (GNT55_29320) - 6230840..6231058 (+) 219 WP_003117240.1 hypothetical protein -
  GNT55_RS29335 (GNT55_29325) - 6231048..6233255 (+) 2208 WP_075116631.1 phage tail protein -
  GNT55_RS29340 (GNT55_29330) - 6233252..6234400 (+) 1149 WP_179063325.1 hypothetical protein -
  GNT55_RS29345 (GNT55_29335) - 6234397..6234684 (+) 288 WP_010791802.1 hypothetical protein -
  GNT55_RS29350 (GNT55_29340) - 6234758..6234982 (+) 225 WP_003121473.1 hypothetical protein -
  GNT55_RS29355 (GNT55_29345) pilP 6235070..6235492 (-) 423 Protein_5831 type 4a pilus biogenesis lipoprotein PilP -
  GNT55_RS29360 (GNT55_29350) pilO 6235489..6236112 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  GNT55_RS29365 (GNT55_29355) pilN 6236109..6236705 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  GNT55_RS29370 (GNT55_29360) pilM 6236705..6237769 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  GNT55_RS29375 (GNT55_29365) - 6237954..6240425 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77438.99 Da        Isoelectric Point: 5.2285

>NTDB_id=404046 GNT55_RS29070 WP_003161249.1 6193063..6195207(-) (pilQ) [Pseudomonas aeruginosa strain SE5416]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
SGYGKDGNIGIKDEDGMNCGPIAGSCTFPTTGTSKSPSPFVDLGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINKN
EVNAKILVNDGETIVIGGVFSNEQSKSVEKVPFLGELPYLGRLFRRDTVTDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=404046 GNT55_RS29070 WP_003161249.1 6193063..6195207(-) (pilQ) [Pseudomonas aeruginosa strain SE5416]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCATTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTACAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGTT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATTCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGATCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAACTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTATCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACCGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGTGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTCCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGTGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGTATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGTCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AGCGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGTAGCTGTAC
CTTCCCGACCACGGGTACCAGCAAGTCGCCGTCGCCGTTCGTCGACCTCGGTGCCAAGGATGCTACCTCCGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTGAAGGGCTCGGAAGTCCCCTACCAGGAGGCCAGTTC
CAGCGGCGCTACCAGCACCTCGTTCAAGGAAGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTCAAGGTAACCAAGGACGCGCCGGATTACCAGAATATGTTGAACGGCGTACCTCCTATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGTAACGAGCAGAGCAAGTC
GGTGGAGAAGGTACCCTTCCTCGGCGAACTGCCTTACCTCGGGCGGTTGTTCCGGCGAGATACCGTCACCGACCGGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(549-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

100

100

1
  pilQ Acinetobacter baumannii D1279779

40.699

100

0.408
  pilQ Acinetobacter baumannii strain A118

40.699

100

0.408
  comQ Acinetobacter baylyi ADP1

41.168

98.319

0.405
  pilQ Legionella pneumophila strain Lp02

38.102

95.938

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.956

95.938

0.364

Multiple sequence alignment