Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GK020_RS01675 Genome accession   NZ_CP046392
Coordinates   361404..363110 (-) Length   568 a.a.
NCBI ID   WP_005181778.1    Uniprot ID   -
Organism   Acinetobacter indicus strain WMB-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356404..368110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GK020_RS01645 - 356552..356875 (+) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -
  GK020_RS01650 rlmB 356981..357733 (+) 753 WP_075167607.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GK020_RS01655 - 357751..358671 (+) 921 WP_045796176.1 DMT family transporter -
  GK020_RS01660 coaE 358668..359276 (-) 609 WP_104989177.1 dephospho-CoA kinase -
  GK020_RS01665 pilD 359287..360147 (-) 861 WP_005181774.1 prepilin peptidase Machinery gene
  GK020_RS01670 pilC 360147..361373 (-) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  GK020_RS01675 pilB 361404..363110 (-) 1707 WP_005181778.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GK020_RS01680 tpiA 363400..364191 (+) 792 WP_104989176.1 triose-phosphate isomerase -
  GK020_RS01685 secG 364205..364534 (+) 330 WP_104989175.1 preprotein translocase subunit SecG -
  GK020_RS01710 rimP 365449..365973 (+) 525 WP_045796174.1 ribosome maturation factor RimP -
  GK020_RS01715 nusA 366006..367490 (+) 1485 WP_005181786.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63758.21 Da        Isoelectric Point: 4.9207

>NTDB_id=403789 GK020_RS01675 WP_005181778.1 361404..363110(-) (pilB) [Acinetobacter indicus strain WMB-7]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAAKKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQKDDDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=403789 GK020_RS01675 WP_005181778.1 361404..363110(-) (pilB) [Acinetobacter indicus strain WMB-7]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCGAAAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACCATTGCTGAAAAAATTTCCTATGAATTTGGTGAACCGATTTTTGACTTGGCGGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTGGATGAAAAACTGTTCACCAAATACCGAATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTGGAAGTTGGCGACAACGCTCCCGAAACACAAAAAGATGACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAGTTACTGGTCGATGCAATCCGTATGGGCGCCTCGGATTTGCACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTGTTGCGTCAAATTGCCAATCCTCCGTTACAACTGGCCACACGGCTCGCCTCACGTTTA
AAAGTTATGTCACAAATGGACATCTCGGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCATTGCCGACCCTGTTCGGGGAAAAAATCGTACTACGTATTCTCGATCCATCTAGCG
CCATGCTGGGGATTGATGCACTTGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTACTGATTACCGGTCCGACCGGTTCCGGTAAAACCGTGTCTTTATATACCGGCTTAAATATCCTCAACCGTGAAGA
CACCAATATTTCTACCGCCGAGGATCCGGTGGAAATTAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGAAAGTGG
GGCTAACCTTTGCCGCAGCACTAAAATCATTCCTGCGTCAGGATCCGGATATTGTCATGGTCGGTGAGATCCGTGATCTG
GAAACTGCAGAAATTGCTATTAAAGCTGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCCGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCCTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTCTGCCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAGCTCGGCTTTACCGAA
GAAGATTTAAACCAGCCAGAATTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGCCGTGAAGGCTATAAAGGCCGTGT
CGGGGTCTATGAAGTAATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGCAATGCCTTACAGATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.806
  pilB Acinetobacter baylyi ADP1

78.07

100

0.783
  pilB Legionella pneumophila strain ERS1305867

50.97

99.824

0.509
  pilF Neisseria gonorrhoeae MS11

51.429

98.592

0.507
  pilB Vibrio cholerae strain A1552

49.647

99.648

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.551

98.063

0.486
  pilB Vibrio campbellii strain DS40M4

50.672

91.725

0.465
  pilF Thermus thermophilus HB27

37.128

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

33.821

100

0.366

Multiple sequence alignment