Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GK020_RS01665 Genome accession   NZ_CP046392
Coordinates   359287..360147 (-) Length   286 a.a.
NCBI ID   WP_005181774.1    Uniprot ID   -
Organism   Acinetobacter indicus strain WMB-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354287..365147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GK020_RS01645 - 356552..356875 (+) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -
  GK020_RS01650 rlmB 356981..357733 (+) 753 WP_075167607.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GK020_RS01655 - 357751..358671 (+) 921 WP_045796176.1 DMT family transporter -
  GK020_RS01660 coaE 358668..359276 (-) 609 WP_104989177.1 dephospho-CoA kinase -
  GK020_RS01665 pilD 359287..360147 (-) 861 WP_005181774.1 prepilin peptidase Machinery gene
  GK020_RS01670 pilC 360147..361373 (-) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  GK020_RS01675 pilB 361404..363110 (-) 1707 WP_005181778.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GK020_RS01680 tpiA 363400..364191 (+) 792 WP_104989176.1 triose-phosphate isomerase -
  GK020_RS01685 secG 364205..364534 (+) 330 WP_104989175.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32048.33 Da        Isoelectric Point: 7.8299

>NTDB_id=403787 GK020_RS01665 WP_005181774.1 359287..360147(-) (pilD) [Acinetobacter indicus strain WMB-7]
MQEFIHYLIQHPTALYFVVGIFSLCIGSFLNVVIYRTPKMMEQEWRNDCQMLLHPEQAIIDESKISLSQPASTCPKCHSP
IRWYQNIPVISWLLLRGKCGQCQNPISIRYPFIELLTAVCALTVVAVFGPTLQMLFGLVLTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTSASSAIWGYIIGFLCLWVVYYLFKLVTGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
VGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGDAIMKLYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=403787 GK020_RS01665 WP_005181774.1 359287..360147(-) (pilD) [Acinetobacter indicus strain WMB-7]
ATGCAAGAATTCATTCATTATCTGATTCAACACCCAACTGCGCTGTATTTCGTAGTTGGGATTTTTAGTTTATGTATTGG
CAGTTTTCTGAATGTGGTGATTTACCGCACGCCAAAAATGATGGAACAGGAATGGCGCAATGACTGTCAAATGCTGCTGC
ATCCTGAACAGGCCATCATTGATGAAAGCAAAATCAGCCTCAGTCAGCCAGCCTCGACCTGTCCGAAATGCCACAGCCCG
ATTCGCTGGTATCAGAATATTCCAGTAATCAGCTGGCTGCTTCTACGTGGCAAATGTGGCCAGTGCCAAAACCCGATCAG
TATTCGTTATCCTTTTATTGAACTGCTGACCGCAGTGTGTGCCTTAACCGTAGTCGCGGTTTTTGGCCCGACCCTGCAAA
TGCTGTTTGGCCTGGTGTTGACCTATGTGTTAATTGCCCTGACCTTTATTGATTTTGACACCCAGCTGCTGCCTGACCGC
TATACCCTCCCCCTGGCTGCACTCGGCTTGGGGGTGAACAGCTATGCCCTGTACACCTCCGCTAGCTCTGCCATTTGGGG
ATATATCATTGGCTTTTTATGTCTGTGGGTGGTGTATTACCTGTTTAAGCTGGTGACTGGCAAAGAAGGTATGGGCTATG
GCGATTTTAAATTGCTGGCCGCCTTAGGTGCCTGGATGGGCCCGCTGATGCTGCCGCTGATTGTGTTGCTATCTTCTCTG
GTTGGTGCAATCATTGGCATTATTTTGCTGAAAATCCGCAAAGAGAACCAGCCCTTTGCCTTTGGCCCTTATATTGCCAT
TGCCGGCTGGATCGCCTTTTTGTGGGGTGATGCAATCATGAAGCTTTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

80

99.65

0.797
  pilD Acinetobacter baumannii D1279779

79.649

99.65

0.794
  pilD Vibrio cholerae strain A1552

55.431

93.357

0.517
  pilD Vibrio campbellii strain DS40M4

53.184

93.357

0.497
  pilD Neisseria gonorrhoeae MS11

47.71

91.608

0.437

Multiple sequence alignment