Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   GL187_RS09890 Genome accession   NZ_CP046379
Coordinates   1930617..1931078 (-) Length   153 a.a.
NCBI ID   WP_000250523.1    Uniprot ID   A0A0B7M7T3
Organism   Streptococcus pneumoniae strain 563     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1925617..1936078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GL187_RS09860 (GL187_09980) - 1925929..1926753 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  GL187_RS09865 (GL187_09985) rnpA 1926728..1927099 (-) 372 WP_000739247.1 ribonuclease P protein component -
  GL187_RS11460 - 1927116..1927247 (-) 132 WP_000768904.1 hypothetical protein -
  GL187_RS09870 (GL187_09990) - 1927248..1928438 (-) 1191 WP_000167765.1 acetate kinase -
  GL187_RS09875 (GL187_09995) comYH 1928489..1929442 (-) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  GL187_RS09880 (GL187_10005) - 1929503..1930090 (-) 588 WP_000679779.1 class I SAM-dependent methyltransferase -
  GL187_RS09885 (GL187_10010) comGG/cglG 1930193..1930639 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  GL187_RS09890 (GL187_10015) comGF/cglF 1930617..1931078 (-) 462 WP_000250523.1 competence type IV pilus minor pilin ComGF Machinery gene
  GL187_RS09895 (GL187_10020) comGE/cglE 1931041..1931343 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  GL187_RS09900 (GL187_10025) comGD/cglD 1931306..1931710 (-) 405 WP_061647912.1 competence type IV pilus minor pilin ComGD Machinery gene
  GL187_RS09905 (GL187_10030) comGC/cglC 1931703..1932029 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  GL187_RS09910 (GL187_10035) comGB/cglB 1932031..1933047 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  GL187_RS09915 (GL187_10040) comGA/cglA/cilD 1932995..1933936 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  GL187_RS09920 (GL187_10045) - 1934011..1934376 (-) 366 WP_000286415.1 DUF1033 family protein -
  GL187_RS09925 (GL187_10050) - 1934527..1935585 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17821.32 Da        Isoelectric Point: 6.4832

>NTDB_id=403712 GL187_RS09890 WP_000250523.1 1930617..1931078(-) (comGF/cglF) [Streptococcus pneumoniae strain 563]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=403712 GL187_RS09890 WP_000250523.1 1930617..1931078(-) (comGF/cglF) [Streptococcus pneumoniae strain 563]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M7T3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae D39

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae R6

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae TIGR4

94.771

100

0.948
  comGF/cglF Streptococcus mitis SK321

93.464

100

0.935
  comGF/cglF Streptococcus mitis NCTC 12261

92.157

100

0.922

Multiple sequence alignment