Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUU84_RS20010 Genome accession   NZ_CP055256
Coordinates   4140535..4141920 (+) Length   461 a.a.
NCBI ID   WP_001025173.1    Uniprot ID   B7LFX4
Organism   Escherichia coli strain AH25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4135535..4146920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUU84_RS19985 (HUU84_19985) aroP 4136083..4137456 (+) 1374 WP_001331235.1 aromatic amino acid transporter AroP -
  HUU84_RS19990 (HUU84_19990) ampE 4137499..4138353 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HUU84_RS19995 (HUU84_19995) ampD 4138350..4138901 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HUU84_RS20000 (HUU84_20000) nadC 4138989..4139882 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUU84_RS20005 (HUU84_20005) pilA 4140085..4140525 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  HUU84_RS20010 (HUU84_20010) pilB 4140535..4141920 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  HUU84_RS20015 (HUU84_20015) hofC 4141910..4143112 (+) 1203 WP_000157234.1 protein transport protein HofC -
  HUU84_RS20020 (HUU84_20020) guaC 4143147..4144190 (-) 1044 WP_001217338.1 GMP reductase -
  HUU84_RS20025 (HUU84_20025) - 4144346..4144390 (-) 45 WP_120795372.1 protein YacM -
  HUU84_RS20030 (HUU84_20030) coaE 4144415..4145035 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  HUU84_RS20035 (HUU84_20035) zapD 4145035..4145778 (+) 744 WP_001194734.1 cell division protein ZapD -
  HUU84_RS20040 (HUU84_20040) yacG 4145788..4145985 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  HUU84_RS20045 (HUU84_20045) mutT 4146085..4146474 (-) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50452.16 Da        Isoelectric Point: 6.4532

>NTDB_id=403028 HUU84_RS20010 WP_001025173.1 4140535..4141920(+) (pilB) [Escherichia coli strain AH25]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLARTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=403028 HUU84_RS20010 WP_001025173.1 4140535..4141920(+) (pilB) [Escherichia coli strain AH25]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAGCTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTGTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGTGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Legionella pneumophila strain ERS1305867

49.738

82.863

0.412
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Vibrio campbellii strain DS40M4

48.32

83.948

0.406
  pilB Vibrio cholerae strain A1552

46.465

85.9

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.632

83.731

0.39
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36

Multiple sequence alignment