Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HUU84_RS20005 Genome accession   NZ_CP055256
Coordinates   4140085..4140525 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain AH25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4135085..4145525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUU84_RS19985 (HUU84_19985) aroP 4136083..4137456 (+) 1374 WP_001331235.1 aromatic amino acid transporter AroP -
  HUU84_RS19990 (HUU84_19990) ampE 4137499..4138353 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HUU84_RS19995 (HUU84_19995) ampD 4138350..4138901 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HUU84_RS20000 (HUU84_20000) nadC 4138989..4139882 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUU84_RS20005 (HUU84_20005) pilA 4140085..4140525 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  HUU84_RS20010 (HUU84_20010) pilB 4140535..4141920 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  HUU84_RS20015 (HUU84_20015) hofC 4141910..4143112 (+) 1203 WP_000157234.1 protein transport protein HofC -
  HUU84_RS20020 (HUU84_20020) guaC 4143147..4144190 (-) 1044 WP_001217338.1 GMP reductase -
  HUU84_RS20025 (HUU84_20025) - 4144346..4144390 (-) 45 WP_120795372.1 protein YacM -
  HUU84_RS20030 (HUU84_20030) coaE 4144415..4145035 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=403027 HUU84_RS20005 WP_000360895.1 4140085..4140525(+) (pilA) [Escherichia coli strain AH25]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=403027 HUU84_RS20005 WP_000360895.1 4140085..4140525(+) (pilA) [Escherichia coli strain AH25]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment