Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FOB89_RS09965 Genome accession   NZ_CP046329
Coordinates   2210056..2211168 (+) Length   370 a.a.
NCBI ID   WP_011919675.1    Uniprot ID   -
Organism   Shewanella putrefaciens strain FDAARGOS_681     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2205056..2216168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB89_RS09930 (FOB89_09930) rdgB 2205151..2205768 (-) 618 WP_011919679.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  FOB89_RS09935 (FOB89_09935) - 2205879..2206313 (-) 435 WP_011788669.1 DUF4426 domain-containing protein -
  FOB89_RS09940 (FOB89_09940) yggU 2206391..2206681 (-) 291 WP_011919678.1 DUF167 family protein YggU -
  FOB89_RS09945 (FOB89_09945) - 2206681..2207229 (-) 549 WP_011919677.1 YggT family protein -
  FOB89_RS09950 (FOB89_09950) proC 2207276..2208094 (-) 819 WP_011919676.1 pyrroline-5-carboxylate reductase -
  FOB89_RS09955 (FOB89_09955) - 2208275..2208973 (-) 699 WP_011788673.1 YggS family pyridoxal phosphate-dependent enzyme -
  FOB89_RS09960 (FOB89_09960) pilT 2209009..2210046 (+) 1038 WP_011788674.1 type IV pilus twitching motility protein PilT Machinery gene
  FOB89_RS09965 (FOB89_09965) pilU 2210056..2211168 (+) 1113 WP_011919675.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FOB89_RS09970 (FOB89_09970) - 2211307..2211846 (+) 540 WP_011919674.1 glutathione peroxidase -
  FOB89_RS09975 (FOB89_09975) hemH 2211879..2212892 (+) 1014 WP_011919673.1 ferrochelatase -
  FOB89_RS09980 (FOB89_09980) ruvX 2212954..2213379 (-) 426 WP_011919672.1 Holliday junction resolvase RuvX -
  FOB89_RS09985 (FOB89_09985) - 2213411..2213974 (-) 564 WP_011788679.1 YqgE/AlgH family protein -
  FOB89_RS09990 (FOB89_09990) yciH 2214087..2214416 (-) 330 WP_011919671.1 stress response translation initiation inhibitor YciH -
  FOB89_RS09995 (FOB89_09995) - 2214582..2214896 (-) 315 WP_011919670.1 hypothetical protein -
  FOB89_RS10000 (FOB89_10000) - 2215075..2215635 (+) 561 WP_011788682.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41125.29 Da        Isoelectric Point: 6.3723

>NTDB_id=402740 FOB89_RS09965 WP_011919675.1 2210056..2211168(+) (pilU) [Shewanella putrefaciens strain FDAARGOS_681]
MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETRIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKEPTGSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=402740 FOB89_RS09965 WP_011919675.1 2210056..2211168(+) (pilU) [Shewanella putrefaciens strain FDAARGOS_681]
ATGGATGTACGTCCGTTTTTAAAAGTCATCGTTGAGCGTAAAGCCTCTGATTTGTTTATTACCGCGGGCTTTCCTCCCAG
TGCAAAAATTGATGGCGAATTACGTCCCCTCGCCGAGAGTGCATTTACGCCTGCACAGTCTTTAGATTTTGTTGAGTCAC
TCATGACTGAAGCGCAGAAACAGGAATTTCACTCAACCCGTGAATGTAACTTTGCTTTTGCGGCAAAGGATCTCGGTCGT
TTCCGAGTCAGCGCTTTTTGGCAAAGGGAATCTCCAGGATGTGTTATGCGCCGGATTGAGACCAGAATTCCCGAAGTCGA
TGACTTAAAATTACCTCCTATTTTAAAAGATCTGGTGATGAGCAAGCGTGGGCTTATCATTATGGTCGGCGGCACTGGTA
CGGGTAAGTCAACCTCATTAGCAGCATTGGTTGGATACCGTAATGCCCATGCCCGTGGCCATATATTGACGATTGAAGAT
CCTGTTGAATTTGTTCACGATCATAGAAAAAGCATTATCACCCAGCGTGAAGTAGGCATAGATACGGAGTCATTCGATGC
GGCATTAAAAAGCTCCCTTCGTCAGGCACCCGATGTGATCTTAATCGGTGAAATTCGTACCCAAGAGACGATGGAATTTG
CATTGTCGTTTGCAGAAACAGGTCATCTTTGTATGGCAACCCTACATGCCAATAATGCGAACCAAGCACTCGATCGGATT
ATGCACTTAGTGCCTGAGAGTAAACATCAGCAGTTACTATTTGATTTATCCTTAAATTTACGTGGTATTGTGGCGCAGCA
ATTGATCCCCAAAGTCGATGGAACGGGGCGCCGCGCCGCGATTGAAGTATTAATTAATACTCCAAGGGTAGCGAGTTTAA
TTGCCAAAAATGAGCTGCATTTACTCAAGGAAACCATGGCAAAATCGAATGAACAGGGGATGCAGACATTCGACCAAGCG
TTACTGAAATTATATGTTGATGGTGAAATTAGTTATGCCGATGCCTTACACCATGCTGACTCACCAAACGATTTACGTTT
GATGATCAAATTACAAAATAAAGAGCCTACTGGTTCAAGTTTTATGGAGGGCGTAACACTCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.215

100

0.605
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362

Multiple sequence alignment