Detailed information NTG0401464

Bioinformatically predicted

Overview

• Name: comFC
• Type: Machinery gene
• Locus tag: GKC27_RS17280
• Genome accession: NZ_CP046130
• Coordinates: 3318859..3319545 (-)
• Length: 228 a.a.
• NCBI ID: WP_003212791.1
• Uniprot ID: -
• Organism: Bacillus pumilus strain DSM 27
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3313859..3324545

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GKC27_RS17245 (GKC27_17055)	fliW	3313992..3314426 (-)	435	WP_003212676.1	flagellar assembly protein FliW	-
GKC27_RS17250 (GKC27_17060)	-	3314455..3315033 (-)	579	WP_003213106.1	DUF6470 family protein	-
GKC27_RS17255 (GKC27_17065)	flgL	3315082..3315993 (-)	912	WP_003213719.1	flagellar hook-associated protein FlgL	-
GKC27_RS17260 (GKC27_17070)	flgK	3316003..3317523 (-)	1521	WP_003213544.1	flagellar hook-associated protein FlgK	-
GKC27_RS17265 (GKC27_17075)	-	3317539..3318021 (-)	483	WP_003213076.1	flagellar protein FlgN	-
GKC27_RS17270 (GKC27_17080)	flgM	3318040..3318303 (-)	264	WP_003213252.1	flagellar biosynthesis anti-sigma factor FlgM	-
GKC27_RS17275 (GKC27_17085)	-	3318383..3318802 (-)	420	WP_003212865.1	TIGR03826 family flagellar region protein	-
GKC27_RS17280 (GKC27_17090)	comFC	3318859..3319545 (-)	687	WP_003212791.1	ComF family protein	Machinery gene
GKC27_RS17285 (GKC27_17095)	-	3319542..3319835 (-)	294	WP_003213271.1	competence protein ComFB	-
GKC27_RS17290 (GKC27_17100)	comFA	3319902..3321281 (-)	1380	WP_269457875.1	DEAD/DEAH box helicase	Machinery gene
GKC27_RS17295 (GKC27_17105)	-	3321401..3322243 (-)	843	WP_003213402.1	DegV family protein	-
GKC27_RS17300 (GKC27_17110)	degU	3322461..3323150 (-)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
GKC27_RS17305 (GKC27_17115)	degS	3323215..3324390 (-)	1176	WP_003213166.1	sensor histidine kinase	Regulator

Sequence

Protein

• Length: 228 a.a.
• Molecular weight: 25993.42 Da
• Isoelectric Point: 9.6636

>NTDB_id=401464 GKC27_RS17280 WP_003212791.1 3318859..3319545(-) (comFC) [Bacillus pumilus strain DSM 27]
MICLICEGHFVASFTWKSLLLLNDDRLCIACKHHLEKIQRPICPACGRAQSNDQLCQDCSMWKERPDSTTVLVKNRSVYA
YNDLMKDVLSRFKFRGDTALAELFKQDVRAVFKRSFSMKEPVLIPIPLSKERLKERGFNQSVILASLIGEPILQPLVKIH
QSKQSKKKKNERLDQKGMFQITQTDAIVQREVILIDDIYTTGATIYDAARILKEAGAKSVSSFTLIRS

Nucleotide

• Length: 687 bp

>NTDB_id=401464 GKC27_RS17280 WP_003212791.1 3318859..3319545(-) (comFC) [Bacillus pumilus strain DSM 27]
TTGATCTGCCTCATTTGTGAGGGACATTTTGTCGCTTCTTTTACATGGAAATCATTACTTCTATTAAATGATGATCGTCT
ATGTATAGCATGTAAGCATCACCTAGAAAAAATTCAACGCCCGATTTGTCCAGCCTGCGGGAGAGCACAGTCGAACGATC
AATTATGTCAAGACTGTTCCATGTGGAAAGAACGCCCTGATTCTACTACAGTACTTGTGAAAAATAGGTCTGTCTATGCT
TACAACGATTTGATGAAAGATGTCCTTTCCCGCTTTAAATTTCGAGGTGATACAGCTTTAGCTGAGCTTTTTAAACAAGA
TGTCCGAGCAGTATTTAAGCGCTCTTTTTCAATGAAAGAACCTGTCCTGATCCCGATTCCTTTAAGTAAAGAAAGACTAA
AGGAAAGAGGGTTTAATCAGTCCGTCATACTTGCTTCCTTGATTGGTGAGCCGATCCTTCAGCCACTTGTAAAAATACAT
CAAAGCAAACAGTCCAAGAAAAAGAAAAATGAACGTTTAGATCAAAAAGGAATGTTTCAAATCACGCAGACAGATGCGAT
CGTTCAAAGGGAAGTTATCTTAATTGACGACATTTATACAACAGGGGCCACCATTTATGATGCGGCGAGAATTTTAAAAG
AAGCAGGTGCCAAAAGTGTTTCCTCTTTTACGTTGATCCGTAGTTAA

Domains

Predicted by InterproScan.

• Phosphoribosyl transferase domain (Pribosyltran) (136-226)

• Double zinc ribbon domain (DZR_2) (3-59)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFC	Bacillus subtilis subsp. subtilis str. 168	50	100	0.504
comFC/cflB	Streptococcus mitis NCTC 12261	38.865	100	0.39
comFC/cflB	Streptococcus mitis SK321	38.865	100	0.39
comFC/cflB	Streptococcus pneumoniae Rx1	38.865	100	0.39
comFC/cflB	Streptococcus pneumoniae D39	38.865	100	0.39
comFC/cflB	Streptococcus pneumoniae R6	38.865	100	0.39
comFC/cflB	Streptococcus pneumoniae TIGR4	38.865	100	0.39