Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   GKC27_RS17290 Genome accession   NZ_CP046130
Coordinates   3319902..3321281 (-) Length   459 a.a.
NCBI ID   WP_269457875.1    Uniprot ID   -
Organism   Bacillus pumilus strain DSM 27     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3314902..3326281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC27_RS17255 (GKC27_17065) flgL 3315082..3315993 (-) 912 WP_003213719.1 flagellar hook-associated protein FlgL -
  GKC27_RS17260 (GKC27_17070) flgK 3316003..3317523 (-) 1521 WP_003213544.1 flagellar hook-associated protein FlgK -
  GKC27_RS17265 (GKC27_17075) - 3317539..3318021 (-) 483 WP_003213076.1 flagellar protein FlgN -
  GKC27_RS17270 (GKC27_17080) flgM 3318040..3318303 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  GKC27_RS17275 (GKC27_17085) - 3318383..3318802 (-) 420 WP_003212865.1 TIGR03826 family flagellar region protein -
  GKC27_RS17280 (GKC27_17090) comFC 3318859..3319545 (-) 687 WP_003212791.1 ComF family protein Machinery gene
  GKC27_RS17285 (GKC27_17095) - 3319542..3319835 (-) 294 WP_003213271.1 competence protein ComFB -
  GKC27_RS17290 (GKC27_17100) comFA 3319902..3321281 (-) 1380 WP_269457875.1 DEAD/DEAH box helicase Machinery gene
  GKC27_RS17295 (GKC27_17105) - 3321401..3322243 (-) 843 WP_003213402.1 DegV family protein -
  GKC27_RS17300 (GKC27_17110) degU 3322461..3323150 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  GKC27_RS17305 (GKC27_17115) degS 3323215..3324390 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  GKC27_RS17310 (GKC27_17120) - 3324612..3325280 (+) 669 WP_003212936.1 YigZ family protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52372.30 Da        Isoelectric Point: 10.1260

>NTDB_id=401465 GKC27_RS17290 WP_269457875.1 3319902..3321281(-) (comFA) [Bacillus pumilus strain DSM 27]
MNMDKAMELGQHLQSRHLLTAETRCSQSDLDWLEEKGLVIRTPAIERKANGLICCRCGVSKNRYFAHSSCDKCQKDCVYC
RSCIMMGKTTECGFLYEWTGPQMEEACHSELTWQGNLSKGQKRASEKLIEAIKNKSDLLIWAVCGAGKTEVLFHGIEYAL
HQGMSVCIATPRTDVVLELEPRLKKAFQGMKIAVLYGGSPQRFQIAPLVIATTHQLMRYKHAFDVLIIDEVDAFPYSIDE
RLQFAVLKAMRKKGVRVYLSATPSKKMIRDVSRGQLEAIKIPLRFHQQPLPVPSFQWIGHWKKKLKKNQLPPKVMNLMQK
HITKKRRVLLFVPSISTMKKVTKVLRKHHLNVEGVSADDPDRKQKVQQFRDYKYDVLVTTTILERGVTISNVQVGVLGAE
STIFTESALVQISGRAGRHPDFFKGDVFFFHFGLTRSMKQAKKHIVKMNDTAEKEFSEK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=401465 GKC27_RS17290 WP_269457875.1 3319902..3321281(-) (comFA) [Bacillus pumilus strain DSM 27]
TTGAACATGGACAAAGCAATGGAACTAGGGCAACACCTGCAGTCACGCCACCTGCTTACTGCTGAAACAAGATGCTCACA
GTCTGATTTGGATTGGCTGGAGGAGAAAGGGTTAGTTATCCGAACACCTGCTATTGAGAGGAAAGCAAATGGTCTTATTT
GCTGCAGATGTGGTGTATCAAAAAATCGGTACTTTGCCCACTCTTCGTGTGACAAGTGTCAAAAAGATTGTGTGTATTGT
AGATCATGCATCATGATGGGGAAAACAACGGAATGTGGCTTTCTTTATGAATGGACGGGTCCACAAATGGAAGAAGCATG
TCACTCGGAATTAACATGGCAGGGAAACCTGTCTAAAGGACAAAAAAGAGCGTCAGAGAAATTGATCGAAGCGATAAAAA
ACAAATCTGATCTACTCATCTGGGCAGTTTGCGGGGCAGGGAAAACAGAGGTGCTTTTTCATGGAATAGAATATGCCTTA
CATCAAGGAATGAGTGTCTGCATTGCGACACCTAGAACCGATGTTGTCCTTGAACTAGAGCCGCGGCTTAAAAAAGCTTT
TCAAGGAATGAAAATAGCTGTTCTTTATGGAGGAAGTCCTCAAAGGTTCCAAATAGCACCGTTAGTAATCGCTACAACTC
ACCAGTTGATGAGATATAAACATGCATTTGATGTGCTCATTATAGATGAAGTCGACGCCTTCCCTTATTCAATCGATGAA
CGTCTCCAATTTGCTGTTTTAAAGGCAATGAGAAAGAAAGGGGTTAGGGTTTATTTAAGTGCGACGCCGTCTAAGAAAAT
GATAAGAGATGTATCTCGTGGACAACTAGAAGCCATAAAAATTCCTCTGCGCTTTCACCAACAACCTTTACCGGTCCCAT
CCTTTCAATGGATTGGACATTGGAAGAAGAAATTAAAAAAGAATCAGCTCCCACCTAAAGTAATGAATTTGATGCAGAAA
CATATTACAAAAAAGAGAAGGGTTTTACTTTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACGAAGGTTCTTAGAAA
GCACCACTTAAATGTAGAAGGGGTGTCTGCTGATGATCCAGATAGGAAACAAAAGGTCCAGCAATTCAGAGATTACAAAT
ACGATGTACTAGTTACAACCACAATTCTAGAAAGAGGTGTAACCATTTCAAATGTGCAAGTGGGGGTACTAGGTGCAGAA
TCTACTATCTTTACAGAAAGTGCACTTGTTCAGATTTCTGGTAGAGCAGGTAGACATCCCGATTTTTTTAAAGGAGATGT
TTTCTTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATTGTAAAAATGAATGATACAGCTGAAA
AAGAGTTCTCCGAAAAATAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(116-264)

Helicase conserved C-terminal domain (Helicase_C)

(314-419)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

54.066

99.129

0.536

Multiple sequence alignment