Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GJQ55_RS00865 Genome accession   NZ_CP046056
Coordinates   181434..182549 (+) Length   371 a.a.
NCBI ID   WP_228345625.1    Uniprot ID   A0A9E8FJT4
Organism   Venatoribacter cucullus strain ASxL5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 176434..187549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJQ55_RS00840 (GJQ55_00845) - 177626..177889 (-) 264 WP_228345620.1 DUF167 family protein -
  GJQ55_RS00845 (GJQ55_00850) - 177916..178509 (-) 594 WP_228345621.1 YggT family protein -
  GJQ55_RS00850 (GJQ55_00855) proC 178605..179423 (-) 819 WP_228345622.1 pyrroline-5-carboxylate reductase -
  GJQ55_RS00855 (GJQ55_00860) - 179466..180170 (-) 705 WP_228345623.1 YggS family pyridoxal phosphate-dependent enzyme -
  GJQ55_RS00860 (GJQ55_00865) pilT 180387..181421 (+) 1035 WP_228345624.1 type IV pilus twitching motility protein PilT Machinery gene
  GJQ55_RS00865 (GJQ55_00870) pilU 181434..182549 (+) 1116 WP_228345625.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GJQ55_RS00870 (GJQ55_00875) - 182643..184919 (+) 2277 WP_228345626.1 bifunctional diguanylate cyclase/phosphodiesterase -
  GJQ55_RS00875 (GJQ55_00880) ideA 184988..185671 (+) 684 WP_228345627.1 endonuclease Regulator
  GJQ55_RS00880 (GJQ55_00885) - 185722..186171 (+) 450 WP_228345628.1 PhnE/PtxC family ABC transporter permease -
  GJQ55_RS00885 (GJQ55_00890) - 186313..187380 (+) 1068 WP_228345629.1 HlyD family secretion protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41230.40 Da        Isoelectric Point: 6.2871

>NTDB_id=400730 GJQ55_RS00865 WP_228345625.1 181434..182549(+) (pilU) [Venatoribacter cucullus strain ASxL5]
MAIDKFLRLMVEKGASDMFITTGVAPSMKVNGKIMPLTKNALSPEQVREVVFGLMNSKQQDEFEEKKELNFAINASGIGR
FRASAFYQRNTCGMVLRRIETRIPGIDQLGLPDVIKEISMAKRGLILFVGGTGTGKSTSLAAMIGHRNKNSKGHILSIED
PIEFIHSHDGCIVTQREVGLDTESFDVALKNCLRQAPDVIMIGEVRTQETMEQAITFAETGHLCLATLHANNANQALERV
IHFFPPERHNQIWMDLSLNLRAIVAQQLIPTPDGRSRRAVVEVLLNTPLVADLIRKGEVHELKPLMAKSSELGMQTFDQA
LYALYEQGEITYEDAIAHADSPNDLRLMIKLGSETDSDHLDGATRNLRLQD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=400730 GJQ55_RS00865 WP_228345625.1 181434..182549(+) (pilU) [Venatoribacter cucullus strain ASxL5]
ATGGCAATTGATAAGTTTCTGCGTCTGATGGTGGAAAAGGGCGCCTCCGATATGTTCATTACTACCGGTGTGGCACCGTC
GATGAAGGTGAACGGTAAGATTATGCCGCTGACCAAAAATGCGCTGTCGCCCGAGCAGGTGCGGGAGGTGGTGTTCGGTT
TAATGAACAGCAAACAGCAGGATGAGTTTGAAGAAAAAAAGGAACTCAACTTCGCCATTAACGCCTCCGGTATCGGCCGT
TTCCGCGCCAGTGCGTTTTATCAGCGCAACACCTGCGGCATGGTGCTGCGGCGCATTGAAACCCGCATTCCCGGCATTGA
TCAGCTTGGCCTGCCTGATGTTATTAAAGAAATTTCCATGGCCAAGCGCGGCCTGATTCTGTTTGTGGGGGGTACCGGTA
CCGGTAAATCCACCTCGCTGGCGGCCATGATCGGCCACCGCAATAAAAACAGTAAGGGCCATATTTTAAGCATCGAAGAT
CCGATTGAATTTATTCACTCGCACGATGGCTGCATTGTCACCCAGCGCGAAGTGGGGCTGGACACCGAAAGCTTCGATGT
GGCACTGAAAAACTGCCTGCGTCAGGCGCCGGATGTGATCATGATCGGTGAGGTGCGGACCCAGGAAACCATGGAGCAGG
CCATTACCTTTGCGGAAACCGGCCACCTCTGTCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGGTG
ATCCACTTTTTCCCGCCGGAGCGACACAACCAGATCTGGATGGATCTGTCGCTGAACCTGCGGGCCATTGTGGCGCAGCA
GCTAATTCCGACCCCGGATGGCCGTTCGCGCCGCGCGGTGGTAGAAGTGTTGCTGAATACACCGCTGGTGGCTGACCTGA
TCCGTAAGGGTGAAGTGCACGAACTGAAACCTTTGATGGCGAAATCCAGTGAGCTGGGTATGCAAACCTTCGACCAGGCC
CTGTATGCCCTGTACGAGCAGGGTGAAATCACTTACGAAGATGCCATCGCCCACGCCGATTCACCCAACGATCTGCGCCT
GATGATCAAACTCGGTTCGGAAACCGACTCGGACCATCTGGACGGCGCCACCCGCAATCTGCGCCTGCAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

74.324

99.73

0.741
  pilU Acinetobacter baylyi ADP1

61.905

96.226

0.596
  pilU Vibrio cholerae strain A1552

54.472

99.461

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.023

92.453

0.407
  pilT Pseudomonas aeruginosa PAK

38.841

92.992

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.027

0.361
  pilT Vibrio cholerae strain A1552

40.12

90.027

0.361

Multiple sequence alignment