Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GJD93_RS12965 Genome accession   NZ_CP046045
Coordinates   2462626..2464338 (+) Length   570 a.a.
NCBI ID   WP_154321112.1    Uniprot ID   -
Organism   Acinetobacter towneri strain 19110F47     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2457626..2469338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJD93_RS12925 (GJD93_12915) nusA 2458161..2459645 (-) 1485 WP_154321110.1 transcription termination factor NusA -
  GJD93_RS12930 (GJD93_12920) rimP 2459681..2460205 (-) 525 WP_154321111.1 ribosome maturation factor RimP -
  GJD93_RS12955 (GJD93_12945) secG 2461214..2461543 (-) 330 WP_004975868.1 preprotein translocase subunit SecG -
  GJD93_RS12960 (GJD93_12950) tpiA 2461557..2462348 (-) 792 WP_004975867.1 triose-phosphate isomerase -
  GJD93_RS12965 (GJD93_12955) pilB 2462626..2464338 (+) 1713 WP_154321112.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GJD93_RS12970 (GJD93_12960) pilC 2464369..2465598 (+) 1230 WP_154321113.1 type II secretion system F family protein Machinery gene
  GJD93_RS12975 (GJD93_12965) pilD 2465622..2466485 (+) 864 WP_154321114.1 prepilin peptidase Machinery gene
  GJD93_RS12980 (GJD93_12970) coaE 2466495..2467094 (+) 600 WP_154321115.1 dephospho-CoA kinase -
  GJD93_RS12985 (GJD93_12975) - 2467100..2467978 (-) 879 WP_218948405.1 DMT family transporter -
  GJD93_RS12990 (GJD93_12980) rlmB 2468073..2468822 (-) 750 WP_154321117.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GJD93_RS12995 (GJD93_12985) - 2469011..2469334 (-) 324 WP_004975854.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63429.61 Da        Isoelectric Point: 4.8519

>NTDB_id=400648 GJD93_RS12965 WP_154321112.1 2462626..2464338(+) (pilB) [Acinetobacter towneri strain 19110F47]
MSVQQATPRFTGFIRRLVEDGLVSAENMQTAIKAAQKDNVDIVPYLIQNLNIAPLIIAEKISNEFGEPIFDLSVYDSALI
VREGIDEKLFSKYNLLPIFKRNHLLYVATSNPTNIEAMDAIRFNSKMNIEAIVVEHDKLVKLIEQNFASESAFEFEDDDF
DLNFEDGNSEPEQAAEDEPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKNKSIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKAPSDIPKQSLLEMGF
TEADLSNPEFQVYQPVGCSECREGYKGRVGVYEVMKVTPEISRIIMEDGNAIQIADASAKSGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=400648 GJD93_RS12965 WP_154321112.1 2462626..2464338(+) (pilB) [Acinetobacter towneri strain 19110F47]
ATGTCAGTACAACAAGCTACACCTAGATTTACAGGTTTTATTCGACGTTTGGTCGAAGATGGGCTCGTTTCTGCTGAAAA
TATGCAAACTGCCATTAAAGCCGCACAAAAAGATAATGTTGATATTGTTCCCTATCTCATTCAAAACCTAAATATCGCGC
CTTTAATCATTGCTGAAAAAATTTCTAATGAGTTTGGCGAACCTATTTTTGATTTAAGCGTGTATGACTCTGCGCTCATC
GTGCGTGAAGGCATTGATGAAAAGCTTTTCAGCAAATATAACCTTTTACCTATTTTTAAGCGCAATCACCTGCTTTACGT
TGCGACCAGTAACCCGACCAATATCGAAGCAATGGATGCCATTCGTTTCAACAGTAAAATGAATATTGAAGCCATTGTGG
TCGAACACGATAAATTGGTAAAACTGATTGAACAAAACTTTGCGAGTGAGTCTGCTTTTGAATTTGAAGATGATGACTTC
GATCTCAACTTTGAAGATGGTAACAGCGAACCAGAACAAGCTGCAGAAGATGAACCGCAGGGTGATGAATCCCCAATTGT
TAAATACATCAACAAATTACTGATTGATGCAATTCGCATGGGTGCTTCGGACTTACACTTTGAACCGTATGAAAAAACCT
ATCGAGTGCGTTACCGTGTCGATGGTGTATTACGTCAAATTGCCACCCCACCCTTACAGTTAGCTAACCGCTTGGCATCA
CGTTTAAAAGTGATGTCCCAAATGGACATTTCTGAAAAGCGTGTCCCGCAAGATGGTCGTATTAAATTAAAACTATCTAA
AAACAAGTCCATCGACTTCCGTGTTAACTCCTTGCCAACCTTGTTCGGTGAAAAAATTGTACTGCGTATTTTAGATCCAT
CTAGCGCCATGCTTGGGATTGATGCCTTGGGTTATGAGCCTGAACAAAAAGCTTTGTTTATGGAAGCTCTGGACAAACCG
CAAGGTATGTTACTGATCACAGGTCCAACAGGTTCGGGTAAAACGGTATCGCTCTATACAGGTTTAAACATTCTAAACCG
AGAAGACACCAACATTTCCACTGCCGAAGACCCTGTGGAAATTAACCTTGAAGGGATCAATCAGGTTAACGTCAACAATA
AAGTTGGTCTGACCTTTGCTGCAGCGCTGAAATCATTCTTACGTCAAGACCCTGACATTGTTATGGTGGGTGAGATTCGT
GACTTAGAAACAGCAGAAATTGCAATTAAAGCAGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAACAGTGC
ACCAGAAACTTTAACCCGTTTACGCAACATGGGCGTTCCTTCCTTTAACATCGCAACTTCGGTAAACCTCGTCATTGCAC
AGCGTTTGGCACGACGCCTATGCCCGCATTGCAAAGCTCCTTCCGATATTCCAAAACAAAGCTTATTGGAAATGGGCTTT
ACTGAAGCCGATTTGAGCAATCCAGAATTTCAGGTCTATCAACCTGTAGGCTGTTCTGAATGCCGTGAAGGTTATAAAGG
ACGTGTTGGTGTTTATGAAGTGATGAAAGTCACACCAGAAATTTCTAGAATTATTATGGAGGATGGTAATGCCATCCAAA
TTGCTGATGCGTCAGCAAAATCGGGGTTTAACAATCTGCGTCGTTCGGGGTTACTTAAGGTCATGCAGGGGGTGACTTCA
TTGCAAGAAGTCAATCGTGTGACCAGTGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.596

100

0.786
  pilB Acinetobacter baylyi ADP1

76.491

100

0.765
  pilB Legionella pneumophila strain ERS1305867

50.172

100

0.511
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.41

99.298

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.214

98.246

0.474
  pilB Vibrio campbellii strain DS40M4

50.482

91.053

0.46
  pilF Thermus thermophilus HB27

37.262

100

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

34.733

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.367

98.596

0.368

Multiple sequence alignment