Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPE50_RS04295 Genome accession   NZ_CP054353
Coordinates   909992..911377 (-) Length   461 a.a.
NCBI ID   WP_001570936.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-172     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 904992..916377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPE50_RS04265 (HPE50_04255) mutT 905440..905838 (+) 399 WP_000736029.1 8-oxo-dGTP diphosphatase MutT -
  HPE50_RS04270 (HPE50_04260) yacG 905927..906124 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  HPE50_RS04275 (HPE50_04265) zapD 906134..906877 (-) 744 WP_001194731.1 cell division protein ZapD -
  HPE50_RS04280 (HPE50_04270) coaE 906877..907497 (-) 621 WP_001269517.1 dephospho-CoA kinase -
  HPE50_RS26995 - 907522..907566 (+) 45 WP_120795372.1 protein YacM -
  HPE50_RS04285 (HPE50_04275) guaC 907722..908765 (+) 1044 WP_001217333.1 GMP reductase -
  HPE50_RS04290 (HPE50_04280) hofC 908800..910002 (-) 1203 WP_000157242.1 protein transport protein HofC -
  HPE50_RS04295 (HPE50_04285) pilB 909992..911377 (-) 1386 WP_001570936.1 type II secretion system protein GspE Machinery gene
  HPE50_RS04300 (HPE50_04290) pilA 911387..911827 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  HPE50_RS04305 (HPE50_04295) nadC 912030..912923 (-) 894 WP_001135178.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPE50_RS04310 (HPE50_04300) ampD 913011..913562 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPE50_RS04315 (HPE50_04305) ampE 913559..914413 (+) 855 WP_000171986.1 beta-lactamase regulator AmpE -
  HPE50_RS04320 (HPE50_04310) aroP 914456..915829 (-) 1374 WP_001330227.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50662.34 Da        Isoelectric Point: 6.4968

>NTDB_id=398945 HPE50_RS04295 WP_001570936.1 909992..911377(-) (pilB) [Escherichia coli strain SCU-172]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSNELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAFQEKHQP
KVELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=398945 HPE50_RS04295 WP_001570936.1 909992..911377(-) (pilB) [Escherichia coli strain SCU-172]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCCCCTTCGAATGAACTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTTTTCAGGAGAAGCATCAGCCC
AAAGTAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAATCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACACTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.685

100

0.419
  pilB Legionella pneumophila strain ERS1305867

50.26

83.297

0.419
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

47.396

83.297

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Acinetobacter baylyi ADP1

45.736

83.948

0.384
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

44.133

85.033

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36