Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GII14_RS02055 Genome accession   NZ_CP045857
Coordinates   419620..421326 (-) Length   568 a.a.
NCBI ID   WP_165564355.1    Uniprot ID   A0A6G7LMS3
Organism   Shewanella chilikensis strain DC57     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 414620..426326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII14_RS02025 (GII14_02020) mutT 415183..415581 (-) 399 WP_101055951.1 8-oxo-dGTP diphosphatase MutT -
  GII14_RS02030 (GII14_02025) yacG 415578..415793 (-) 216 WP_039033599.1 DNA gyrase inhibitor YacG -
  GII14_RS02035 (GII14_02030) zapD 415838..416578 (-) 741 WP_165564352.1 cell division protein ZapD -
  GII14_RS02040 (GII14_02035) coaE 416575..417186 (-) 612 WP_165564353.1 dephospho-CoA kinase -
  GII14_RS02045 (GII14_02040) pilD 417187..418101 (-) 915 WP_165564354.1 A24 family peptidase Machinery gene
  GII14_RS02050 (GII14_02045) pilC 418176..419444 (-) 1269 WP_101055955.1 type II secretion system F family protein Machinery gene
  GII14_RS02055 (GII14_02050) pilB 419620..421326 (-) 1707 WP_165564355.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GII14_RS02060 (GII14_02055) - 421519..421890 (+) 372 Protein_380 helix-turn-helix domain-containing protein -
  GII14_RS22470 (GII14_02060) - 421829..422287 (-) 459 WP_165564356.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GII14_RS02070 (GII14_02065) - 422356..422736 (+) 381 WP_165564357.1 hypothetical protein -
  GII14_RS02075 (GII14_02070) nadC 423277..424137 (-) 861 WP_101055958.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GII14_RS02080 (GII14_02075) ampD 424322..424867 (+) 546 WP_226565745.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GII14_RS02085 (GII14_02080) ampE 424921..425772 (+) 852 WP_101055959.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62859.87 Da        Isoelectric Point: 5.2480

>NTDB_id=398682 GII14_RS02055 WP_165564355.1 419620..421326(-) (pilB) [Shewanella chilikensis strain DC57]
MPTTGLHLGLSTLFIRKGLLNESSLTEAIKQSQNSNQALVTTLVTSKLLSARSIAELCYEEYGTPLLDLNEFDLSSIPDE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKVLEEDLSSLDIDGLDEES
LAGIEVTDTDKRPEDNSEGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMRLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYMEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQAEEVPEHELLQLGFS
QQQIDSGFTVYKPCGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EVNRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=398682 GII14_RS02055 WP_165564355.1 419620..421326(-) (pilB) [Shewanella chilikensis strain DC57]
ATGCCAACTACCGGCCTCCATTTAGGATTATCGACGCTATTTATCCGTAAGGGGCTGCTCAATGAGAGCAGCCTGACGGA
GGCCATCAAGCAGTCGCAAAACAGCAACCAAGCCCTGGTCACCACCCTGGTGACCAGCAAGCTTTTATCTGCCCGCAGCA
TAGCCGAGCTCTGTTATGAGGAGTACGGCACCCCGCTGCTGGATCTCAATGAGTTTGACCTCAGCAGCATTCCGGATGAG
TTTCTCAACAAGAAACTGATCGACAAACACAGATGTCTGCCGCTGTTCAAGCGCGGCAACCGCCTCTATATCGCCACCTC
GGATCCCACCAATATCGCCGCCCTGGAAGACTTTCAGTTCAGCGCCGGCCTGCATGCCGAGGCGATACTGGTCGAAGAGG
ACAAGTTAGCCAAGGCGCTGGAAAAGGTACTGGAAGAGGACTTGAGCTCGCTGGATATCGATGGCCTGGATGAAGAGTCC
CTCGCCGGCATTGAAGTCACAGATACCGACAAGCGCCCGGAAGACAACAGCGAAGGCAAGGATGACGCCCCCATCGTCAT
CTATATCAATAAAATTCTCACCGATGCCATCCGTAAGGGCGCATCGGATCTGCATTTTGAACCCTACGAGAAGCGCTACC
GCATCCGTTTTCGTATCGATGGCATACTCCATGAGGTGTCCGAGCCGCCGATTTCACTTTCCGGACGGATATCGGCTCGC
TTAAAGGTGATGTCCAAGCTGGATATCGCCGAGCGCCGGGTGCCCCAGGATGGCCGTATCAAGATGAGGCTGTCGCGCAA
CAAGAGCATCGACTTTCGGGTCAGTACCCTGCCTACCCTCTGGGGCGAAAAGATAGTGATGCGGATCCTCGACTCCTCTT
CGGCGCAGTTGGGCATAGAAAAGCTCGGCTATGAGGAAGACCAGAAACAAGCCTATATGGAGATGCTGGCAAGACCCCAG
GGGATGATCCTGGTTACTGGCCCCACGGGTTCGGGGAAAACCGTGTCCCTCTATACCGGCCTCAATATTCTCAATACCGA
GGAGCGCAATATCAGCACCGCCGAAGATCCGGTGGAGATTAACCTCGAAGGGGTTAACCAGGTGCATATCAACCCCAAGG
CCGGGTTAACCTTCGCCTCGGCGCTGCGCTCCTTTCTGCGTCAGGATCCGGACGTGGTGATGGTGGGGGAAATTCGTGAC
CTGGAAACCGCCGAAATCGCCATCAAGGCGGCCCAAACCGGCCACTTGGTGCTCTCGACCCTGCACACCAACTCATCGGC
CGAAACCCTCACCCGTTTGCTCAATATGGGCGTACCCGGCTACAACATTGCCAGTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGTCTCTGTCCCGAATGCCGACAAGCGGAAGAGGTCCCCGAACATGAACTGCTGCAGCTGGGCTTCAGC
CAGCAACAGATAGATAGCGGTTTTACCGTTTATAAGCCCTGCGGCTGTGAGCACTGCTCCGGTGGCTATAAGGGCAGGGT
CGGTATCTATGAGGTGATGAAGATGTCCGATGAAATTGCCAGAACCATTATGGAAGGCGGTAATTCACTGCAGATTGCCG
CTACCGCCAAGCAGCAGGGAATGAGGGACTTGCGCCTGTCTGGCCTGCTTAAAGTCATTCAGGGCGTCACCAGCATTGCC
GAGGTCAACCGGGTAACCAGTTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7LMS3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.824

98.768

0.581
  pilB Acinetobacter baumannii D1279779

57.932

98.768

0.572
  pilB Legionella pneumophila strain ERS1305867

55.654

99.648

0.555
  pilB Vibrio parahaemolyticus RIMD 2210633

52.982

100

0.532
  pilB Vibrio cholerae strain A1552

53.506

95.423

0.511
  pilB Vibrio campbellii strain DS40M4

51.332

99.12

0.509
  pilF Neisseria gonorrhoeae MS11

48.759

99.296

0.484
  pilF Thermus thermophilus HB27

37.968

98.768

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.129

97.887

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

41.283

87.852

0.363

Multiple sequence alignment