Detailed information NTG0398680

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GII14_RS02045
• Genome accession: NZ_CP045857
• Coordinates: 417187..418101 (-)
• Length: 304 a.a.
• NCBI ID: WP_165564354.1
• Uniprot ID: A0A6G7LMS2
• Organism: Shewanella chilikensis strain DC57
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 412187..423101

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GII14_RS02025 (GII14_02020)	mutT	415183..415581 (-)	399	WP_101055951.1	8-oxo-dGTP diphosphatase MutT	-
GII14_RS02030 (GII14_02025)	yacG	415578..415793 (-)	216	WP_039033599.1	DNA gyrase inhibitor YacG	-
GII14_RS02035 (GII14_02030)	zapD	415838..416578 (-)	741	WP_165564352.1	cell division protein ZapD	-
GII14_RS02040 (GII14_02035)	coaE	416575..417186 (-)	612	WP_165564353.1	dephospho-CoA kinase	-
GII14_RS02045 (GII14_02040)	pilD	417187..418101 (-)	915	WP_165564354.1	A24 family peptidase	Machinery gene
GII14_RS02050 (GII14_02045)	pilC	418176..419444 (-)	1269	WP_101055955.1	type II secretion system F family protein	Machinery gene
GII14_RS02055 (GII14_02050)	pilB	419620..421326 (-)	1707	WP_165564355.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GII14_RS02060 (GII14_02055)	-	421519..421890 (+)	372	Protein_380	helix-turn-helix domain-containing protein	-
GII14_RS22470 (GII14_02060)	-	421829..422287 (-)	459	WP_165564356.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GII14_RS02070 (GII14_02065)	-	422356..422736 (+)	381	WP_165564357.1	hypothetical protein	-

Sequence

Protein

• Length: 304 a.a.
• Molecular weight: 33834.42 Da
• Isoelectric Point: 7.8945

>NTDB_id=398680 GII14_RS02045 WP_165564354.1 417187..418101(-) (pilD) [Shewanella chilikensis strain DC57]
MSDFIAILSHNLWLFLVLCFLFASVIGSFLNVVIHRLPVMMKRDWQQECNQYLQEYQPEFAKQIGEAKLNAPIDNYPAKY
NLLLPKSACPKCGAHIKPWHNLPIIGYLMLGGKCAACKTKISARYPLFELLTALLICTLAWHFGPTLQFAYATILTFGLI
ALSGIDLDEMLLPDQITLPLLWLGLLINISGTFASITDATIGAAAGYLSLWSIYWLFKLITGKEGMGYGDFKLMALFGAW
LGWQMLPLIILLSSLVGALVGVLLIVFKNLNRGNPIPFGPYIAIAGWVAMVWGQQIVDWYLGTL

Nucleotide

• Length: 915 bp

>NTDB_id=398680 GII14_RS02045 WP_165564354.1 417187..418101(-) (pilD) [Shewanella chilikensis strain DC57]
ATGTCAGACTTTATTGCCATATTGAGCCACAATCTGTGGCTCTTTCTTGTTCTCTGTTTTCTGTTTGCCTCTGTGATAGG
CAGCTTTCTCAATGTGGTAATCCACCGTCTGCCGGTAATGATGAAGCGCGATTGGCAGCAGGAATGCAATCAATACCTGC
AGGAATACCAACCCGAATTCGCCAAACAGATAGGTGAAGCCAAGCTCAATGCACCGATAGATAACTACCCGGCCAAGTAC
AATCTGTTGCTGCCGAAGTCTGCCTGCCCCAAGTGCGGCGCTCATATCAAACCCTGGCACAACTTACCCATAATCGGTTA
CCTGATGCTTGGCGGCAAATGCGCTGCCTGTAAGACCAAGATCTCAGCAAGATATCCTCTGTTCGAGTTGTTGACTGCCC
TACTCATTTGTACGCTCGCTTGGCACTTTGGTCCAACACTGCAGTTTGCCTATGCAACTATTCTGACCTTTGGACTGATA
GCCCTCAGCGGCATAGATTTGGATGAGATGCTGTTGCCTGACCAGATAACCCTGCCGCTGCTGTGGCTCGGTTTACTGAT
TAATATCTCCGGCACTTTTGCCAGCATTACCGATGCCACCATAGGCGCCGCAGCCGGTTATTTAAGCCTCTGGTCTATCT
ACTGGCTGTTCAAATTAATCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCAAGCTGATGGCTCTATTCGGCGCCTGG
CTGGGCTGGCAGATGCTGCCACTGATAATTCTGCTCTCTTCTTTGGTGGGCGCCCTGGTAGGTGTGTTACTCATAGTGTT
CAAGAACCTCAATCGGGGTAACCCCATTCCCTTTGGCCCCTATATTGCCATAGCCGGTTGGGTTGCCATGGTGTGGGGGC
AACAGATAGTTGACTGGTACTTGGGAACACTTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-143)

• Type IV leader peptidase family (Peptidase_A24) (154-262)

3D structure

• AlphaFold DB: A0A6G7LMS2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.536	96.382	0.497
pilD	Acinetobacter nosocomialis M2	49.338	99.342	0.49
pilD	Acinetobacter baumannii D1279779	49.007	99.342	0.487
pilD	Vibrio campbellii strain DS40M4	48.197	100	0.484
pilD	Neisseria gonorrhoeae MS11	45.552	92.434	0.421